Nanoscale Biocatalysis

This Methods in Molecular Biology(TM) series book outlines processes in nanoscale technology-enabled biocatalysis, including preparation of dynamic protein structures, and methodologies with potential for scale-up preparation of nano-structured biocatalysts.

Nanoscale science and engineering, which deal with size-dependent properties and phenomenon at nanometer scale, are unveiling new mechanisms that scientists must rely on heavily at the present time to achieve efficient and sustainable chemical processing technologies. In Nanoscale Biocatalysis: Methods and Protocols , expert researchers in the field contribute detailed methodologies and procedures that have been developed from recent research in this burgeoning area of nanoscale technology-enabled biocatalysis. The volume opens with concepts in preparing unique and dynamic protein structures for biocatalysis, then moves on to cover methods for preparation of enzyme assembles or complexes that maintain molecular-like Brownian mobility, the development of protein-nanostructure complexes using carbon nanotubes (CNTs) and nanoparticles, as well as methodologies that have great potential for scale-up preparation of nano-structured biocatalysts. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Nanoscale Biocatalysis: Methods and Protocols is an ideal guide to the new wave of development in nearly all the major areas of science and engineering brought about by this fascinating and greatly promising area of study.

Explores the cutting-edge realities of nanoscale technology-enabled biocatalysis Features detailed and practical protocols for working in this burgeoning field Includes expert tips and key implementation advice Includes supplementary material: sn.pub/extras

Autorentext
Ping Wang, 1957 in Shanghai geboren und 1985 in die USA ausgereist, lebt heute als Schriftstellerin in New York. Im Mittelpunkt ihrer literarischen Arbeiten, einem Roman, eine Sammlung von Kurzgeschichten und Gedichte, steht die scharfsichtige Beschreibung ihrer Erfahrungen mit chinesischer und nordamerikanischer Kultur.

Inhalt

Nanoscale Engineered Cytochrome P450 System with a Branch Structure.- Chemically Induced Self-Assembly of Enzyme Nanorings.- Self Assemblies of Polymer-Enzyme Conjugates at Oil-Water Interfaces for Interfacial Biocatalysis.- Molecular Assembly-Assisted Biocatalytic Reactions in Ionic Liquids.- Organic-Soluble Enzyme Nano-Complexes Formed by Ion-Pairing with Surfactants.- Enzyme-Immobilized CNT Network Probe for In vivo Neurotransmitter Detection.- Kinesin I ATP-ase Manipulates Biohybrids Formed from Tubulin and Carbon Nanotubes.- Reversible His-Tagged Enzyme Immobilization on Functionalized Carbon Nanotubes as Nanoscale Biocatalyst.- A TiO 2 Nanoparticle System for Sacrificial Solar H 2 Production Prepared by Rational Combination of a Hydrogenase with a Ruthenium Photosensitizer.- Preparation and Characterization of Single Enzyme Nanogels.- Fabrication and Characterization of Bioactive Thiol-Silicate Nanoparticles.- Immobilization of Enzymes on Fumed Silica Nanoparticles for Applications in Nonaqueous Media.- Microencapsulation of Bioactive Nanoparticles.- Engineering the Logical Properties of a Genetic AND Gate.- Strain Engineering Strategies for Improving Whole-Cell Biocatalysis: Engineering Escherichia coli to Overproduce Xylitol as an Example.- Enzyme-Carrying Electrospun Nanofibers.- Uniform Lab-Scale Biocatalytic Nanoporous Latex Coatings for Reactive Microorganisms.- Entrapment of Enzymes in Nanoporous Sol-Gels.