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This volume organizes and collects technical advances from leaders in the field to make laboratory protocols more readily available and understandable to those who are attempting to incorporate nanotechnologic techniques into their proteomic research.

As two relatively new fields of study, proteomics and nanotechnology have developed in parallel with each other to allow an increased precision in the identification of post-translational protein modifications as well as to provide a more automated isolation and detection of rare proteins in both serum and tissues. The Nanoproteomics: Methods and Protocols volume organizes and collects technical advances from leaders in the field to make laboratory protocols more readily available and understandable to those who are attempting to incorporate nanotechnologic techniques into their proteomic research. Conveniently divided into five sections, this detailed volume covers preliminary sample preparation, nanoscale fluidic devices and methods, nanostructured surfaces and nanomaterials, and nanoproteomic techniques to detect and understand protein and proteomic alterations specific to human pathology. Written in the highly successful series entitled Methods in Molecular Biology™, these chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step laboratory protocols that are readily reproducible, and tips on troubleshooting and avoiding known pitfalls.

Convenient and authoritative, Nanoproteomics: Methods and Protocols offers key procedures that are culled from the laboratories of leaders in the field of nanoproteomics with the aim of helping researchers in their standardization and proliferation of protocols that will lead to a more wide scale adoption and smoother progress in this vital field.

Attempts to standardize and proliferate these key procedures in order to lead to wide scale adoption

Contains easy-to-follow methods and protocols, ready for the lab

Features expert tips and implementation advice to ensure successful results


Part I: Preliminary Sample Preparation

1. MALDI-TOF Serum Profiling Using Semi-Automated Serum Peptide Capture with Magnetic Reversed Phase (C18) Beads

Jaco C. Knol and Connie R. Jimenez

2. Proteomics of Epithelial Lining Fluid Obtained by Bronchoscopic Microprobe Sampling

Lorenza Franciosi, Natalia Govorukhina, Nick ten Hacken, Dirkje Postma, and Rainer Bischoff

Part II: Nanoscale Separation

3. Protein Identification Using Nano-HPLC-MS: ESI-MS and MALDI-MS Interfaces

Rui Vitorino, Jana Krenkova, Frantisek Foret, Pedro Domingues, and Francisco Amado

4. Three Dimensional Peptide Fractionation for Highly Sensitive Nanoscale LC-Based Shotgun Proteomic Analysis of Complex Protein Mixtures

Sricharan Bandhakavi, Todd W. Markowski, Hongwei Xie, and Timothy J. Griffin

5. Nanospray Ion Mobility Mass Spectrometry of Selected High Mass Species

Iain Campuzano and Kevin Giles

6. Nanoelectrospray-MSn of Native and Permethylated Glycans

Christina Bleckmann, Hildegard Geyer, and Rudolf Geyer

7. N -linked Global Glycan Profiling by NanoLC Mass Spectrometry

Michael S. Bereman and David C. Muddiman

8. Applications of Nanoscale Liquid Chromatography Coupled to Tandem Mass Spectrometry in Quantitative Studies of Protein Expression, Protein-Protein Interaction, and Protein Phosphorylation

Metodi V. Metodiev

9. Nano LC-MS/MS: A Robust Setup for Proteomic Analysis

Marco Gaspari and Giovanni Cuda

10. Nanofluidic Devices for Rapid Continuous-Flow Bioseparation

Pan Mao and Jianping Fu

11. Quantifying Attomole Amounts of Proteins from Complex Samples by Nano-LC and Selected Reaction Monitoring (SRM)

Thomas Fröhlich and Georg J. Arnold

Part III: Nanomaterials: Nanostructured Surfaces

12. A Sample Preparation Method for Gold Nanoparticle-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

Yen-Hsiu Lin and Wei-Lung Tseng

13. Nanostructured TiO2 Thin Films for Phosphoproteomics Studies with MALDI Mass Spectrometry

Federico Torta, Matteo Fusi, Carlo S.Casari, Andrea Li Bassi, and Angela Bachi

14. Nanofilament Silicon for Matrix-Free Laser Desorption/Ionization Mass Spectrometry

Chia-Wen Tsao and Don L. DeVoe

15. Protein Nanoarrays for High Resolution Patterning of Bacteria on Gold Surfaces

Cait Costello, Jan-Ulrich Kreft, Christopher M. Thomas, and Paula M. Mendes

Part IV: Nanomaterials: Nanoscale Tools for Proteomic Discovery

16. Engineered Multifunctional Nanotools for Biological Applications

Mohammed Ibrahim Shukoor, Muhammad Nawaz Tahir, Thomas Schladt, Wolfgang Tremel, Zhiqun Zhang, Kevin K. Wang, and Firas Kobeissy

17. The Selective Capture of Phosphopeptides by Zirconium Phosphonate-Modified Magnetic Nanoparticles

Liang Zhao, Ren'an Wu, and Hanfa Zou

18. Nanowire Biosensors for Label-Free, Real-Time, Ultrasensitive Protein Detection

Gengfeng Zheng and Charles M. Lieber

19. Generation of Anti-Infectome/Anti-Proteome Nanobodies

Gholamreza Hassanzadeh-Ghassabeh, Dirk Saerens, and Serge Muyldermans

Part V: Nanotechnology: Techniques in Proteomics--Potential Application for Health & Disease

20. Isolation, Propagation, and Analysis of Biological Nanoparticles

Michael P. Linnes, Farooq A. Shiekh, Larry W. Hunter, Virginia M. Miller, and John C. Lieske

21. Functionalized Soluble Nanopolymers for Phosphoproteome Analysis

Anton Iliuk, Keerthi Jayasundera, Rachel Schluttenhofer, and W. Andy Tao

22. Elucidating Structural Dynamics of Integral Membrane Proteins on Native Cell Surface by Hydroxyl Radical Footprinting and NanoLC-MS/MS

Yi Zhu, Tiannan Guo, and Siu Kwan Sze

23. Application of Electrostatic Repulsion Hydrophilic Interaction Chromatography (ERLIC) to the Characterization of Proteome, Glycoproteome, and Phosphoproteome Using Nano LC-MS/MS

Piliang Hao, Huoming Zhang, and Siu Kwan Sze


Titel: Nanoproteomics
Untertitel: Methods and Protocols
EAN: 9781493957996
ISBN: 1493957996
Format: Kartonierter Einband
Herausgeber: Humana Press
Anzahl Seiten: 336
Gewicht: 634g
Größe: H254mm x B178mm x T18mm
Jahr: 2016
Untertitel: Englisch
Auflage: Softcover reprint of the original 1st ed. 2011

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