Multiple Myeloma

This volume provides a thorough guide of the study of multiple myeloma (MM)from sample acquisition to the performance of molecular tests, and also discusses difficulties that arise during quality control and interpretation of results. The chapters in this book cover topics, such as next-generation-flow-based methods for detection of minimal residual disease and circulating tumor cells; Cytometry by Time-of-Flights (CyTOF); a droplet digital PCR approach for finding contamination in patients' samples; genome-wide investigation of the methylation and hydroxymethylation status of cytosines; and a microfluidic approach for creating nanoparticles. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Multiple Myeloma: Methods and Protocols is a valuable resource for molecular scientists and laboratory supervisors working with MM samples, and any investigator conducting studies in this field.

Includes cutting-edge methods and protocols Provides a step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt
ELDA qASO-PCR for High Sensitivity Detection of Tumor Cells in Bone Marrow and Peripheral Blood.- EuroFlow-Based Next-Generation Flow Cytometry for Detection of Circulating Tumor Cells and Minimal Residual Disease in Multiple Myeloma.- Cytoplasmic Immunoglobulin vs DNA Analysis by Flow Cytometry.- Deep Profiling of the Immune System of Multiple Myeloma Patients by Cytometry by Time of Flight (CyTOF).- Fluorescence In Situ Hybridization (FISH) in Multiple Myeloma.- Whole Exome Sequencing in Multiple Myeloma to Identify Somatic Single Nucleotide Variants and Key Translocations Involving Immunoglobulin Loci and MYC.- RNA-Sequencing from Low-Input Material in Multiple Myeloma for Application in Clinical Routine.- Protocol for M3P: A Comprehensive and Clinical Oriented Targeted Sequencing Panel for Routine Molecular Analysis in Multiple Myeloma.- Analysis of Circulating Tumor DNA.- Detection of Cross-Sample Contamination in Multiple MyelomaSamples and Sequencing Data.- Analysis of Global Gene Expression Profiles.- Genome Wide Mapping of Methylated and Hydroxyl-Methylated Cytosines using a Modified HpaII Tiny Fragment Enrichment by Ligation Mediated PCR Tagged Sequencing Protocol.- A Rapid and Robust Protocol for Reduced Representation Bisulfite Sequencing in Multiple Myeloma.- Microfluidic Production and Application of Lipid Nanoparticles for Nucleic Acid Transfection.- Microfluidic Assembly of Liposomes with Tunable Size and Co-Loading Capabilities.