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Molecular Genetics of Asthma

  • Fester Einband
  • 292 Seiten
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Presenting topics from the basic application of molecular genetics to more complex gene expression analysis using different models... Weiterlesen
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Beschreibung

Presenting topics from the basic application of molecular genetics to more complex gene expression analysis using different models of study, this detailed volume explores asthma through the lens of genetics, considered to play an essential role in the etiopathogenesis of the disease. Since asthma is a complex disease, this book is designed to provide a review of the most useful techniques with examples of their applications in specific laboratory protocols. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Molecular Genetics of Asthma serves as an ideal guide to researchers investigating this vital field of study.



Includes cutting-edge methods and protocols involving the molecular genetics of asthma

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts



Klappentext

Presenting topics from the basic application of molecular genetics to more complex gene expression analysis using different models of study, this detailed volume explores asthma through the lens of genetics, considered to play an essential role in the etiopathogenesis of the disease.  Since asthma is a complex disease, this book is designed to provide a review of the most useful techniques with examples of their applications in specific laboratory protocols. Written in the highly successfulMethods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Molecular Genetics of Asthma serves as an ideal guide to researchers investigating this vital field of study.



Inhalt
1. Applications of Molecular Genetics to the Study of Asthma Catalina S. Sanz Lozano, Virginia García-Solaesa, Ignacio Dávila González, and María Isidoro-García 2. Assessment and Validation of New Genetic Variants: A systematic In Silico Approach Belén García Berrocal, Asunción García-Sánchez, and María Isidoro-García 3. Introduction to the Gene Expression Analysis Ignacio San Segundo-Val and Catalina S. Sanz Lozano 4. Real Time PCR for Gene Expression Quantification in Asthma Ignacio San Segundo-Val, Virginia García-Solaesa, and Asunción García-Sánchez 5. Validation of Reference Genes in mRNA Expression Analysis Applied to the Study of Asthma Ignacio San Segundo-Val and Catalina S. Sanz Lozano 6. Review of Methods to Study Gene Expression Regulation Applied to Asthma Asunción García-Sánchez and Fernando Marqués García 7. Interactions of DNA and Proteins: Electrophoretic Mobility Shift Assay in Asthma Virginia García-Solaesa and Catalina S. Sanz Lozano 8. SDS-Polyacrylamide Electrophoresis and Western Blotting Applied to the Study of Asthma Virginia García-Solaesa and Sara Ciria Abad 9. Chromatin Immunoprecipitation: Application to the Study of Asthma Asunción García-Sánchez and Fernándo Marqués-García 10. Gene Silencing Delivery Methods: Lipid-Mediated and Electroporation Transfection Protocols Asunción García-Sánchez and Fernando Marqués-García 11. Protocols for Exosome Isolation and RNA Profiling Fernando Marqués-García and María Isidoro-García 12. Cell Culture Techniques: Corticosteroid Treatment in A549 Human Lung Epithelial Cell Elena Marcos-Vadillo and Asunción García-Sánchez 13. Protocol for Lipid-Mediated Transient Transfection in A549 Epithelial Lung Cell Line Elena Marcos-Vadillo and Asunción García-Sánchez 14. Promoter Assay Using Luciferase Reporter Gene in the A549 Cell Line Elena Marcos-Vadillo and Asunción García-Sánchez 15. Review of Mouse Models Applied to the Study of Asthma Fernando Marqués-García and Elena Marcos-Vadillo 16. General and Specific Mouse Models for Asthma Research Fernando Marqués-García and Elena Marcos-Vadillo 17. Mouse Models applied to the Research of Pharmacological Treatments in Asthma Fernando Marqués-García and Elena Marcos-Vadillo 18. Review on Pharmacogenetics and Pharmacogenomics Applied to the Study of Asthma Almudena Sánchez-Martín, Asunción García-Sánchez, and María Isidoro-García

Produktinformationen

Titel: Molecular Genetics of Asthma
Editor:
EAN: 9781493936502
ISBN: 1493936506
Format: Fester Einband
Herausgeber: Springer New York
Genre: Medizin
Anzahl Seiten: 292
Gewicht: 751g
Größe: H260mm x B183mm x T21mm
Jahr: 2016
Auflage: 1st ed. 2016

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