Molecular Embryology

In Molecular Embryology: Methods and Protocols, Second Edition , expert investigators provide a comprehensive guide to the cutting-edge methods used across the dramatically growing field of vertebrate molecular embryology. Time-tested techniques take advantage of the most commonly used vertebrate experimental models: murine embryos for their genetics, chick embryos for in vivo manipulation, zebrafish for mutagenesis, amphibian embryos, and nonvertebrate chordates. The second edition collects classic protocols which have become standard techniques in the laboratory and presents them in a complementary fashion with novel and emerging approaches, allowing researcher to become more familiar with commonly studied embryos used in biomedical research. Insightful to the experienced professional, Molecular Embryology: Methods and Protocols, Second Edition , presents cutting-edge findings of perhaps the greatest period in growth and productivity in the field of developmental biology.

Provides an excellent source for understanding the strengths and weaknesses of the various vertebrate model organisms Comprehensive guide to the cutting-edge methods amongst the dramatically growing field of vertebrate molecular embryology Experts in the field thoroughly describe protocols and techniques accompanied by illustrations New approaches for efficient production of transgenic zebrafish and microarrays

Klappentext

In Molecular Embryology: Methods and Protocols, Second Edition, expert investigators provide a comprehensive guide to the cutting-edge methods used across the dramatically growing field of vertebrate molecular embryology. Time-tested techniques take advantage of the most commonly used vertebrate experimental models: murine embryos for their genetics, chick embryos for in vivo manipulation, zebrafish for mutagenesis, amphibian embryos, and nonvertebrate chordates. The second edition collects classic protocols which have become standard techniques in the laboratory and presents them in a complementary fashion with novel and emerging approaches, allowing researcher to become more familiar with commonly studied embryos used in biomedical research. Insightful to the experienced professional, Molecular Embryology: Methods and Protocols, Second Edition, presents cutting-edge findings of perhaps the greatest period in growth and productivity in the field of developmental biology.

Inhalt
The Mouse Embryo.- The Mouse as a Developmental Model.- Culture of Postimplantation Mouse Embryos.- Organ Culture in the Analysis of Tissue Interactions.- Treatment of Mice with Retinoids In Vivo and In Vitro.- Analysis of Skeletal Ontogenesis through Differential Staining of Bone and Cartilage.- Cell Grafting and Labeling in Postimplantation Mouse Embryos.- Production of Transgenic Rodents by the Microinjection of Cloned DNA into Fertilized One-Celled Eggs.- Cre Recombinase Mediated Alterations of the Mouse Genome Using Embryonic Stem Cells.- Gene Trapping in Mouse Embryonic Stem Cells.- Application of lacZ Transgenic Mice to Cell Lineage Studies.- Transgenic RNA Interference to Investigate Gene Function in the Mouse.- Mouse Primordial Germ Cells.- Gene Transfer to the Rodent Embryo by Retroviral Vectors.- The Chicken Embryo.- The Avian Embryo.- Chick Embryos.- New Culture.- EC Culture.- Grafting Hensen's Node.- Grafting of Somites.- Microsurgical Manipulation of the Notochord.- Transplantation of Avian Neural Tissue.- Grafting of Apical Ridge and Polarizing Region.- Tissue Recombinations in Collagen Gels.- QuailChick Chimeras.- Using Fluorescent Dyes for Fate Mapping, Lineage Analysis, and Axon Tracing in the Chick Embryo.- Gene Transfer in Avian Embryos Using Replication-Competent Retroviruses.- Electroporation in Avian Embryos.- Amphibian Embryos.- An Overview of Xenopus Development.- Mesoderm Induction Assays.- Experimental Embryological Methods for Analysis of Neural Induction in the Amphibian.- A Method for Generating Transgenic Frog Embryos.- Axolotl/Newt.- Zebrafish And Medaka.- The Zebrafish.- Small-Scale Marker-Based Screening for Mutations in Zebrafish Development.- Microinjection and Cell Transplantation in Zebrafish Embryos.- Recent Advances inMeganuclease-and Transposon-Mediated Transgenesis of Medaka and Zebrafish.- Retinoids in Nonmammalian Embryos.- Nonvertebrate Chordates.- Protochordates.- Molecular Techniques.- Subtractive Hybridization and Construction of cDNA Libraries.- Differential Display of Eukaryotic mRNA.- Using DNA Microarrays.- Profiling Gene Transcription in the Developing Embryo.- RT-PCR on Embryos Using Degenerate Oligonucleotide Primers.- Single-Cell RT-PCR cDNA Subtraction.- In-Situ Hybridization of Radioactive Riboprobes to RNA in Tissue Sections.- In-Situ Hybridization and Immunohistochemistry in Whole Embryos.- Wholemount In Situ Hybridization to Xenopus Embryos.- Wholemount In Situ Hybridization to Amphioxus Embryos.- In-Situ Hybridization to Sections (Nonradioactive).- Immunohistochemistry Using Polyester Wax.- Immunohistochemistry on Whole Embryos.- Whole Embryo Assays for Programmed Cell Death.- Protein Techniques.- Microscopy and Photography.- Microscopy and Photomicrography Techniques.