Mitophagy

This volume focuses on mitophagy providing methods and protocols on the methodologies and the background information on monitoring mitophagy. Chapters detail the analysis of mitophagy in connection with Parkinson's disease, especially young onset Parkinson's disease impaired by PINK/parkin pathway, Drosophila, yeast, and Caenorhabditis elegans models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Mitophagy: Methods and Protocols aims to stimulate and encourage researchers to carry out ongoing and new investigations into mitophagy.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt

Short Overview of PINK1/Parkin-mediated Mitophagy.- Induction of PINK1/Parkin-Mediated Mitophagy.- Immunocytochemical Monitoring of PINK1/Parkin-mediated Mitophagy in Cultured Cells.- The Use Of Correlative Light-electron Microscopy (CLEM) To Study PINK1/Parkin-mediated Mitophagy.- Observation Of Parkin-mediated Mitophagy in Pancreatic Beta-cells.- Monitoring Mitochondrial Changes by Alteration of the PINK1-Parkin Signaling in Drosophila.- Assessment of Mitophagy in iPS Cell-derived Neurons.- Investigation of Yeast Mitophagy with Fluorescence Microscopy and Western Blotting.- Mitopho860 Assay as a Tool to Quantitatively Measure Mitophagy Activity.- Mitophagy in Yeast: A Screen of Mitophagy-Deficient Mutants.- Flow Cytometer Monitoring of Bnip3 and Bnip3L/Nix-dependent Mitophagy.- Exploring Micrornas on Nix-Dependent Mitophagy.- Monitoring of Atg5-Independent Mitophagy.- Monitoring of Paternal Mitochondrial Degradation InCaenorhabditis Elegans.- Detection of Hypoxia-induced and Iron Depletion-induced Mitophagy in Mammalian Cells.- Monitoring Mitophagy during Ageing in Caenorhabditis Elegans.- Iron-Starvation Induced Mitophagy in Yeast.