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Microchip-Based Assay Systems

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Beschreibung

This volume explores recent progress in the microelectronics arena, the
resultant miniaturization of component device features to nanometer
size particles, and the ensuing growth in the development and use of
microchip-based techniques in leading laboratories around the world.
While the area of microelectronics is faced with daunting challenges,
it promises fascinating new advances that may translate into products
such as sensors and devices that use nanometer-sized building blocks
for real-world applications. The contributors to this volume are part
of growing interdisciplinary efforts among pioneers in
microelectronics, nanoscience, and health that will continue to create
exciting new microchip-based applications across a variety of sectors.
In the 16 chapters of this volume, leading scientists present
technically precise microchip-based assays for a variety of
applications.

Highlights include: methods for microchip electrophoresis for DNA
separation; fabrication of porous polymer monoliths in microfluidic
chips for selective nucleic acid concentration and purification; rapid
electrical lysis of bacterial cells in a microfluidic device; an
on-chip bioassay that uses immobilized sensing bacteria in a
three-dimensional microfluidic network; and impedimetric detection for
DNA hybridization within microfluidic biochips. Molecular diagnostic
and immunologic protocols include microchip-based enumeration of human
white blood cells; microchip diagnosis of cervical cancer; protocols
for DNA microchips toward molecular signatures in cervical cancer; a
microchip based assay for Interleukin-6; and methods for
microchip-based electrochemical enzyme immunoassays. Several chapters
are devoted to topics in microarray technology, including: applications
of functional protein microarrays; allergen microarrays for the
diagnosis of specific IgE in a multiplex biochip-based immunoassay;
protein-array based multiplexed cytokine assays; and lectin microarrays
for glycoprotein assays.



DNA microchips for diagnosis and molecular signatures in cervical cancer

Cutting-edge microarray applications

Integrated protocols utilizing microfluidic chips, devices, and 3-D networks

Identification of protein-protein interactions in an array format

Methods for the detection of HIV RNA interactions with peptides

Microchip-based electrochemical enzyme immunoassays



Autorentext
In this volume, leading scientists present technically precise microchip-based assays for a variety of applications. Coverage includes methods for microchip electrophoresis for DNA separation, an on-chip bioassay that uses immobilized sensing bacteria in a three-dimensional microfluidic network, and impedimetric detection for DNA hybridization within microfluidic biochips. In addition, the book also covers such molecular diagnostic and immunologic protocols as microchip-based enumeration of human white blood cells, microchip diagnosis of cervical cancer, and protocols for DNA microchips toward molecular signatures in cervical cancer. Several chapters are also devoted to topics in microarray technology, including applications of functional protein microarrays, protein-array based multiplexed cytokine assays, and lectin microarrays for glycoprotein assays.


Klappentext

Microchip-Based Assay Systems explores recent progress in the microelectronics arena, the resultant miniaturization of component device features to nanometer size particles, and the ensuing growth in the development and use of microchip-based techniques in leading laboratories around the world. While the area of microelectronics faces daunting challenges, the field also promises fascinating new advances that may translate into products such as sensors and devices that use nanometer-sized building blocks for real-world applications. The contributors to this volume are part of growing interdisciplinary efforts among pioneers in microelectronics, nanoscience, and health that will continue to create exciting new microchip-based applications across a variety of sectors. In the 16 chapters of this volume, leading scientists present technically precise microchip-based assays for a variety of applications. The protocols detailed in this volume will help foster interest in microchip-based assays by providing readers with all the tools necessary to create their own microchip-based assays targeted to new applications. Certainly, Microchip-Based Assay Systems will help to expand the scope and accelerate the transition of microchip-based assays from academic and industrial research and development centers to real-world use.



Zusammenfassung

This volume explores recent progress in the microelectronics arena, the
resultant miniaturization of component device features to nanometer
size particles, and the ensuing growth in the development and use of
microchip-based techniques in leading laboratories around the world.
While the area of microelectronics is faced with daunting challenges,
it promises fascinating new advances that may translate into products
such as sensors and devices that use nanometer-sized building blocks
for real-world applications. The contributors to this volume are part
of growing interdisciplinary efforts among pioneers in
microelectronics, nanoscience, and health that will continue to create
exciting new microchip-based applications across a variety of sectors.
In the 16 chapters of this volume, leading scientists present
technically precise microchip-based assays for a variety of
applications.

Highlights include: methods for microchip electrophoresis for DNA
separation; fabrication of porous polymer monoliths in microfluidic
chips for selective nucleic acid concentration and purification; rapid
electrical lysis of bacterial cells in a microfluidic device; an
on-chip bioassay that uses immobilized sensing bacteria in a
three-dimensional microfluidic network; and impedimetric detection for
DNA hybridization within microfluidic biochips. Molecular diagnostic
and immunologic protocols include microchip-based enumeration of human
white blood cells; microchip diagnosis of cervical cancer; protocols
for DNA microchips toward molecular signatures in cervical cancer; a
microchip based assay for Interleukin-6; and methods for
microchip-based electrochemical enzyme immunoassays. Several chapters
are devoted to topics in microarray technology, including: applications
of functional protein microarrays; allergen microarrays for the
diagnosis of specific IgE in a multiplex biochip-based immunoassay;
protein-array based multiplexed cytokine assays; and lectin microarrays
for glycoprotein assays.



Inhalt
Table of Contents / Microchip-based Assay Systems / Ed. Pierre N. Floriano Microchip Electrophoresis for DNA Separation by Wire-Imprinted Microchannels on PMMA Substrates Shu-Hui Chen Fabrication of Porous Polymer Monoliths in Microfluidic Chips for Selective Nucleic Acid Concentration and Purification Jay A. A. West, Brent C. Satterfield Rapid Electrical Lysis of Bacterial Cells in a Microfluidic Device Hsiang-Yu Wang, Padmapriya P. Banada, Dr. Arun Bhunia , Chang Lu On-Chip Bioassay Using Immobilized Sensing Bacteria in Three-Dimensional Microfluidic Network Hirofumi Tani, Koji Maehana, Tamio Kamidate Microchip-based Enumeration of Human White Blood Cells Pierre Floriano, Shelley Acosta, Nick Christodoulides, John T. McDevitt Microchip for the Diagnosis of Cervical Cancer Anja Gulliksen, Frank Karlsen DNA Microchips Towards Molecular Signatures in Cervical Cancers Yick F. Wong, Tony K.H. Chung, Vivian W. Wang, David I. Smith Impedimetric-Detection for DNA Hybridization within Microfluidic Biochips Louise Lingerfelt, James Karlinsey, James P. Landers, Anthony Guiseppi-Elie Applications of Functional Protein Microarrays: Identifying Protein-Protein Interactions in an Array Format Matthew A. Coleman, Peter T. Beernink, Julio A. Camarero, Joanna S Albala A Microchip-based Assay for Interleukin-6 Nicolaos Christodoulides, Prya Dharsham, Jorge Wong, Pierre N. Floriano , Dean Neikirk , John T. McDevitt Allergen Microarrays for the Diagnosis of Specific IgE Against Components of Cow milk and Hen Egg in a Multiplex Biochip-based Immunoassay Christian Harwanegg, Sabine Hutter, Reinhard Hiller Surface Plasmon Resonance Imaging on Polypyrrole Protein Chips: Application to Streptavidin Immobilization and Immunodetection Emilie MERCEY, Ludivine GROSJEAN, Andre Roget, ThierryLIVACHE Protein Array-based Multiplexed Cytokine Assays Cheng C. Wang Lectin Microarrays for Glycoprotein Analysis Lara K. Mahal, Kanoelani Pilobello Interaction of HIV RNA with Peptides Detected by Acoustic Shear Wave Sensor Operated in an On-line Format Anil Deisingh, Satie Siewah , Nardos Tassew, Michael Thompson Microchip-based Electrochemical Enzyme Immunoassays Madhu Prakash Chatrathi, Greg Collins, Joseph Wang

Produktinformationen

Titel: Microchip-Based Assay Systems
Untertitel: Methods and Applications
Editor:
EAN: 9781588295880
ISBN: 1588295885
Format: Fester Einband
Herausgeber: Humana Press
Anzahl Seiten: 244
Gewicht: 580g
Größe: H241mm x B160mm x T20mm
Jahr: 2007
Auflage: 2007

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