Microbial-Based Biopesticides

This volume focuses on the developmental areas of biopesticides: production, formulation, application and field efficacy. Chapters guide readers through methods and techniques on environmental, mammalian, safety, and registration. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Microbial-Based Biopesticides aims to ensure successful results in the further study of this vital field.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt

What are Microbial-based Biopesticides?.- Isolation and Mass Production of Trichoderma.- Methods for the Evaluation of the Bioactivity and Biocontrol Potential of Species of Trichoderma.- Purification of the Yersinia Entomophaga Yen-TC Toxin Complex using Size Exclusion Chromatography.- Coated Solid Substrate Microbe Formulations: Pseudomonas spp. and zeolite.- Production of Conidia by the Fungus Metarhizium Anisopliae using Solid-state Fermentation.- Liquid Culture Production of Fungal Microsclerotia.- Isolation and Assessment of Stability of Six Formulations of Entomopathogenic Beauveria Bassiana.- Cell Culture for Production of Insecticidal Viruses.- Formulation of nematodes.- In vivo Production of Entomopathogenic Nematodes.- Detection and Quantification of the Entomopathogenic Fungal Endophyte Beauveria Bassiana in Plants by Nested and Quantitative PCR.- Plant Tissue Preparation for the Detection of an Endophytic Fungus in Planta.- Measuring Chitinase and Protease Activity in Fungal Cultures.- Analytical Methods for Secondary Metabolite Detection.- Development of Biopesticides and Future Opportunities.