Microarray Methods for Drug Discovery

Authoritative and cutting-edge, this book provides a single volume reference for all types of microarrays. It supplies new information on microarrays for cytogenetics and drug metabolism, and covers a wide range of microarray platforms.

While the utilization of microarrays for gene expression studies has been widely published, Microarray Methods for Drug Discovery describes the use of this technology for a multitude of other vital applications. Covering the field of drug discovery through its various aspects, including high throughput screening, target identification, drug metabolism and toxicity screening as well as clinical sample handling, this volume also addresses new emerging fields like miRNA profiling, ribonomic and glycomic profiling. All chapters, contributed by experts in the field, follow the highly successful Methods in Molecular Biology(TM) series format, which is comprised of brief introductions, lists of required materials and reagents, readily reproducible, step-by-step lab protocols, along with detailed tips on troubleshooting and avoiding known pitfalls, making this book an easy read for investigators at all levels of expertise.

Authoritative and cutting-edge, Microarray Methods for Drug Discovery provides an ideal desk reference for scientists who wish to use microarray technology in order to better understand a variety of biological interactions in their respective fields.

Supplies new information on microarrays for cytogenetics and drug metabolism Covers a wide range of microarray platforms used in genomics, epigenomics, genotyping, proteomics, and small molecule screening A good reference for designing microarray experiments to decipher transcriptome regulation and DNA modifications Includes supplementary material: sn.pub/extras

Inhalt
Multicenter Clinical Sample Collection for Microarray Analysis.- Isolation of Total RNA from Transgenic Mouse Melanoma Subsets Using Fluorescence-Activated Cell Sorting.- Microarray Analysis of Embryonic Stem Cells and Differentiated Embryoid Bodies.- Determination of Alternate Splicing Events Using the Affymetrix Exon 1.0 ST Arrays.- Profiling microRNA Expression with the Illumina BeadChip Platform.- TaqMan® Array Cards in Pharmaceutical Research.- DMET™ Microarray Technology for Pharmacogenomics-Based Personalized Medicine.- The Use of Microarray Technology for Cytogenetics.- PCR/LDR/Universal Array Platforms for the Diagnosis of Infectious Disease.- RIP-CHIP in Drug Development.- ChIPing Away at Global Transcriptional Regulation.- HELP (HpaII Tiny Fragment Enrichment by Ligation-Mediated PCR) Assay for DNA Methylation Profiling of Primary Normal and Malignant B Lymphocytes.- High-Throughput Screening of Metalloproteases Using Small Molecule Microarrays.- Metabolic Enzyme Microarray Coupled with Miniaturized Cell-Culture Array Technology for High-Throughput Toxicity Screening.- Use of Tissue Microarray to Facilitate Oncology Research.- Small Molecule Selectivity and Specificity Profiling Using Functional Protein Microarrays.- Production and Application of Glycan Microarrays.