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Metabolic Profiling

  • Kartonierter Einband
  • 404 Seiten
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This book presents protocols that are illustrative of the evolution of metabolic profiling from single molecule analysis to global... Weiterlesen
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Beschreibung

This book presents protocols that are illustrative of the evolution of metabolic profiling from single molecule analysis to global metabolome profiling. It features cutting-edge protocols, presented from the perspective of leading experts in the field.


At the intersection of metabolite analysis, metabolic fingerprinting, and metabolomics, the study of metabolic profiling has evolved steadily over the course of time as have the methods and technologies involved in its study. In Metabolic Profiling: Methods and Protocols, expert researchers in the field present protocols that are illustrative of the evolution of metabolic profiling from single molecule analysis to global metabolome profiling. Comprised of the most essential techniques, this volume covers topics from inborn errors of metabolism and drug metabolite analysis to nuclear magnetic resonance metabolic profiles. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Metabolic Profiling: Methods and Protocols serves as a resource for both established and new investigators in this vital and ever-developing field.

Illustrates the evolution of metabolic profiling from single molecule profiling to global metabolome profiling

Features cutting-edge protocols, presented from the perspective of leading experts in the field

Includes valuable notes highlighting tips on troubleshooting and avoiding known methodological pitfalls



Inhalt
1. Origins of Metabolic Profiling Arthur B. Robinson and Noah E. Robinson 2. Amino Acid Profiling for the Diagnosis of Inborn Errors of Metabolism Monique Piraud, Séverine Ruet, Sylvie Boyer, Cécile Acquaviva, Pascale Clerc-Renaud, David Cheillan, and Christine Vianey-Saban 3. Acylcarnitines: Analysis in Plasma and Whole Blood Using Tandem Mass Spectrometry David S. Millington and Robert D. Stevens 4. Analysis of Organic Acids and Acylglycines for the Diagnosis of Related Inborn Errors of Metabolism by GC- and HPLC-MS Giancarlo la Marca and Cristiano Rizzo 5. HPLC Analysis of Purines and Pyrimidines for the Clinical Biochemical Diagnosis of Related Inborn Errors of Metabolism Giuseppe Lazzarino, Angela Maria Amorini, Valentina Di Pietro, and Barbara Tavazzi 6. Bile Acid Analysis in Various Biological Samples Using Ultra Performance Liquid Chromatography/Electrospray Ionization-Mass Spectrometry (LC/ESI-MS) Masahito Hagio, Megumi Matsumoto, and Satoshi Ishizuka 7. Analysis of Glycolytic Intermediates with Ion Chromatography- and Gas Chromatography-Mass Spectrometry Jan C. van Dam, Cor Ras, and Angela ten Pierick 8. Analysis of the Citric Acid Cycle Intermediates Using Gas Chromatography-Mass Spectrometry Rajan S. Kombu, Henri Brunengraber, and Michelle A. Puchowicz 9. Quantification of Pentose Phosphate Pathway (PPP) Metabolites by Liquid Chromatography-Mass Spectrometry (LC-MS) Amber Jannasch, Miroslav Sedlak, and Jiri Adamec 10. High Performance Liquid Chromatography-Mass Spectrometry (HPLC-MS)-Based Drug Metabolite Profiling Ian D. Wilson 11. Gas Chromatography-Mass Spectrometry (GC-MS)-Based Metabolomics Antonia Garcia and Coral Barbas 12. The Use of Two Dimensional Gas Chromatography-Time-of-Flight-Mass Spectrometry (GCxGC-TOF-MS) for Metabolomic Analysis of Polar Metabolites Kimberly Ralston-Hooper, Amber Jannash, Jiri Adamec, and Maria Sepúlveda 13. LC-MS-Based Metabolomics Sunil Bajad and Vladimir Shulaev 14. Capillary Electrophoresis-Electrospray Ionization-Mass Spectrometry (CE-ESI-MS)-Based Metabolomics Philip Britz-McKibbin 15. Liquid Chromatography-Mass Spectrometry (LC-MS)-Based Lipidomics for Studies of Body Fluids and Tissues Heli Nygren, Tuulikki Seppänen-Laakso, Sandra Castillo, Tuulia Hyötyläinen, and Matej Orei 16. Electrospray Ionization Tandem Mass Spectrometry (ESI-MS/MS)-Based Shotgun Lipidomics Giorgis Isaac 17. Processing and Analysis of GC/LC-MS-Based Metabolomics Data Elizabeth Want and Perrine Masson 18. Nuclear Magnetic Resonance (NMR)-Based Drug Metabolite Profiling Eva M. Lenz 19. Nuclear Magnetic Resonance (NMR)-Based Metabolomics Hector C. Keun and Toby J. Athersuch 20. Slow Magic Angle Sample Spinning: A Non- or Minimal-Invasive Method for High Resolution 1H Nuclear Magnetic Resonance (NMR) Metabolic Profiling Jian Zhi Hu 21. Processing and Modeling of Nuclear Magnetic Resonance (NMR) Metabolic Profiles Timothy M.D. Ebbels, John C. Lindon, and Muireann Coen

Produktinformationen

Titel: Metabolic Profiling
Untertitel: Methods and Protocols
Editor:
EAN: 9781493958160
ISBN: 149395816X
Format: Kartonierter Einband
Herausgeber: Humana Press
Anzahl Seiten: 404
Gewicht: 757g
Größe: H254mm x B178mm x T21mm
Jahr: 2016
Auflage: Softcover reprint of the original 1st ed. 2011

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