Mesenchymal Stem Cell Assays and Applications

This book shows how to propagate, characterize, and engineer this special cell type. Using the Methods in Molecular Biology(TM) format, each chapter offers an introduction, step-by-step methods, materials list, and tips on troubleshooting and avoiding pitfalls.

Mesenchymal Stem Cells have seen an unprecedented level of interest in the last decade, primarily due to their relative ease of isolation, the large numbers of cells present in the adult, and the ability to propagate these cells in culture. In Mesenchymal Stem Cell Assays and Applications, expert researchers from across the globe explore the latest techniques to propagate, characterize, and engineer this special cell type. Chapters outline a set of protocols and assays used by leading investigators in the field, providing standards that can be applied by all researchers to the population of cells used in their experiments. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Ground-breaking and current, Mesenchymal Stem Cell Assays and Applications is a necessary handbook for all researchers working with this ambiguous population of cells.

Includes thirty-five detailed chapters from leading experts around the world Describes cutting-edge methods to isolate, propagate, characterize, and engineer mesenchymal stem cells Highlights a set of optimized protocols to facilitate the use of standardized methods throughout the mesenchymal stem cell research field Includes supplementary material: sn.pub/extras

Inhalt
Mesenchymal Stem Cell Assays and Applications.- Isolation and Expansion of Mesenchymal Stem Cells/Multipotential Stromal Cells from Human Bone Marrow.- Standardized Isolation of Human Mesenchymal Stromal Cells with Red Blood Cell Lysis.- Isolation and Growth of Adipose Tissue-Derived Stem Cells.- Isolation, Culture and Characterization of Human Umbilical Cord Stroma-derived Mesenchymal Stem Cells.- The Isolation and Culture of Human Cord Blood-Derived Mesenchymal Stem Cells Under Low Oxygen Conditions .- Amniotic and Placental Mesenchymal Stem Cell Isolation and Culture.- Manufacture of Clinical Grade Human Placenta-Derived Multipotent Mesenchymal Stromal Cells.- A Method to Isolate and Culture Expand Human Dental Pulp Stem Cells.- Isolation and Culture of Human Multipotent Stromal Cells from the Pancreas.- Derivation and Characterization of Human ESC-Derived Mesenchymal Stem Cells.- Isolation and Culture of Rodent Bone Marrow-Derived Multipotent Mesenchymal Stromal Cells.- Cryopreservation and Revival of Mesenchymal Stromal Cells.- Dynamic Expansion Culture for Mesenchymal Stem Cells.- Ex-Vivo Expansion of Human Mesenchymal Stem Cells on Microcarriers.- Osteogenic Differentiation of Human Multipotent Mesenchymal Stromal Cells.- Assays of Osteogenic Differentiation by Cultured Human Mesenchymal Stem Cells.- Bioreactor Cultivation of Functional Bone Grafts.- Adipogenic Differentiation of Human Mesenchymal Stem Cells.- Chondrogenic Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells: Tips and Tricks.- Usage of Human Mesenchymal Stem Cells as Alternative Source of Smooth Muscle Cells in Vessel Engineering.- Dopaminergic Neuronal Differentiation Protocol for Human Mesenchymal Stem Cells.- Hepatic Differentiation of Mesenchymal Stem Cells: in vitro Strategies.- Hepatic Transplantation of Mesenchymal Stem Cells in Rodent Animal Models.- Phenotypic Analysis and Differentiation of Murine Mesenchymal Stem Cells.- Immunohistochemical Analysis of Human Mesenchymal Stem Cells Differentiating into Chondrogenic, Osteogenic and Adipogenic Lineages.- Panel Development for Multicolor Flow Cytometry Testing of Proliferation and Immunophenotype in hMSCs.- Simplified PCR Assay for Detecting Early Stages of Multipotent Mesenchymal Stromal Cell Differentiation.- Transcriptome Analysis of Common Gene Expression in Human Mesenchymal Stem Cells Derived from Four Different Origins.- Comparison of Microarray and Quantitative Real-Time PCR Methods for Measuring MicroRNA Levels in MSC Cultures.- Two Dimensional Gel Electrophoresis Analysis of Mesenchymal Stem Cells .- Proteomic Analysis of Human Mesenchymal Stem Cells.- Metabolic Labeling and Click Chemistry Detection of Glycoprotein Markers of Mesenchymal Stem Cell Differentiation.- BacMam-Mediated Gene Delivery Into Multipotent Mesenchymal Stromal Cells.- Cell Surface Engineering of Mesenchymal Stem Cells.