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Mesenchymal Stem Cell Assays and Applications

  • Kartonierter Einband
  • 552 Seiten
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This book shows how to propagate, characterize, and engineer this special cell type. Using the Methods in Molecular Biology(TM) fo... Weiterlesen
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This book shows how to propagate, characterize, and engineer this special cell type. Using the Methods in Molecular Biology(TM) format, each chapter offers an introduction, step-by-step methods, materials list, and tips on troubleshooting and avoiding pitfalls.

Mesenchymal Stem Cells have seen an unprecedented level of interest in the last decade, primarily due to their relative ease of isolation, the large numbers of cells present in the adult, and the ability to propagate these cells in culture. In Mesenchymal Stem Cell Assays and Applications, expert researchers from across the globe explore the latest techniques to propagate, characterize, and engineer this special cell type. Chapters outline a set of protocols and assays used by leading investigators in the field, providing standards that can be applied by all researchers to the population of cells used in their experiments. Composed in the highly successful Methods in Molecular Biology(TM) series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Ground-breaking and current, Mesenchymal Stem Cell Assays and Applications is a necessary handbook for all researchers working with this ambiguous population of cells.


Mesenchymal Stem Cell Assays and Applications
Editors: Mohan C. Vemuri, Lucas G. Chase and Mahendra S. Rao

Table of Contents



Part 1-Introduction
1. Mesenchymal Stem Cell Assays and Applications
Mohan C. Vemuri, Lucas G. Chase and Mahendra S. Rao

Part 2- Isolation and Expansion of MSCs from Various Sources

2. Isolation and Expansion of Mesenchymal Stem Cells/Multipotential Stromal Cells from Human Bone Marrow
Patrice Penfornis and Radhika Pochampally

3. Standardized Isolation of Human Mesenchymal Stromal Cells with Red Blood Cell Lysis
Patrick Horn, Simone Bork and Wolfgang Wagner

4. Isolation and Growth of Adipose Tissue-Derived Stem Cells
Vladimir Zachar, Jeppe Grøndahl Rasmussen and Trine Fink

5. Isolation, Culture and Characterization of Human Umbilical Cord Stroma-derived Mesenchymal Stem Cells
Alp Can and Deniz Balci

6. The Isolation and Culture of Human Cord Blood-Derived Mesenchymal Stem Cells Under Low Oxygen Conditions
Anita Laitinen, Johanna Nystedt and Saara Laitinen

7. Amniotic and Placental Mesenchymal Stem Cell Isolation and Culture
Justin D. Klein and Dario O. Fauza

8. Manufacture of Clinical Grade Human Placenta-Derived Multipotent Mesenchymal Stromal Cells
Nina Ilic, Gary Brooke, Patricia Murray, Sarah Barlow, Tony Rossetti, Rebecca Pelekanos, Sonia Hancock and Kerry Atkinson

9. A Method to Isolate and Culture Expand Human Dental Pulp Stem Cells Stan Gronthos, Agnieszka Arthur, P. Mark Bartold and Songtao Shi

10. Isolation and Culture of Human Multipotent Stromal Cells from the Pancreas
Karen L. Seeberger, Alana Eshpeter, and Gregory S. Korbutt

11. Derivation and Characterization of Human ESC-Derived Mesenchymal Stem Cells
Ruenn Chai Lai, Andre Choo and Sai Kiang Lim

12. Isolation and Culture of Rodent Bone Marrow-Derived Multipotent Mesenchymal Stromal Cells
Nance Beyer Nardi and Melissa Camassola

13. Cryopreservation and Revival of Mesenchymal Stromal Cells
Mandana Haack-Sørensen and Jens Kastrup

14. Dynamic Expansion Culture for Mesenchymal Stem Cells
Hicham Majd, Thomas M. Quinn, Pierre-Jean Wipff, and Boris Hinz

15. Ex-Vivo Expansion of Human Mesenchymal Stem Cells on Microcarriers
Francisco dos Santos, Pedro Z. Andrade, Gemma Eibes, Cláudia Lobato da Silva and Joaquim M.S. Cabral

Part 3- MSC Lineage Differentiation and Analysis

16. Osteogenic Differentiation of Human Multipotent Mesenchymal Stromal Cells
Deepak M. Gupta, Nicholas J. Panetta and Michael T. Longaker

17. Assays of Osteogenic Differentiation by Cultured Human Mesenchymal Stem Cells
Ulf Krause, Anja Seckinger and Carl A. Gregory

18. Bioreactor Cultivation of Functional Bone Grafts
Warren L. Grayson, Sarindr Bhumiratana, Christopher Cannizzaro and Gordana Vunjak-Novakovic

19. Adipogenic Differentiation of Human Mesenchymal Stem Cells
Trine Fink and Vladimir Zachar
20. Chondrogenic Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells: Tips and Tricks.
Luis A. Solchaga, Kitsie J. Penick and Jean F. Welter

21. Usage of Human Mesenchymal Stem Cells as Alternative Source of Smooth Muscle Cells in Vessel Engineering
Zhaodi Gong and Laura E. Niklason

22. Dopaminergic Neuronal Differentiation Protocol for Human Mesenchymal Stem Cells
Katarzyna A. Trzaska and Pranela Rameshwar

23. Hepatic Differentiation of Mesenchymal Stem Cells: in vitro Strategies
Sarah Snykers, Joery De Kock, Vanhaecke Tamara and Vera Rogiers

24. Hepatic Transplantation of Mesenchymal Stem Cells in Rodent Animal Models
Bruno Christ, Sandra Brückner and Peggy Stock

25. Phenotypic Analysis and Differentiation of Murine Mesenchymal Stem Cells
Lindolfo da Silva Meirelles and Dimas Tadeu Covas

Part 4- MSC Phenotypic Characterization and Extended Applications

26. Immunohistochemical Analysis of Human Mesenchymal Stem Cells Differentiating into Chondrogenic, Osteogenic and Adipogenic Lineages
Zheng Yang, Jacqueline Frida Schmitt and Eng Hin Lee

27. Panel Development for Multicolor Flow Cytometry Testing of Proliferation and Immunophenotype in hMSCs
Jolene A. Bradford and Scott T. Clarke

28. Simplified PCR Assay for Detecting Early Stages of Multipotent Mesenchymal Stromal Cell Differentiation
Shayne E. Boucher

29. Transcriptome Analysis of Common Gene Expression in Human Mesenchymal Stem Cells Derived from Four Different Origins
Tzu-Hao Wang, Yun-Shien Lee, Shiaw-Min Hwang

30. Comparison of Microarray and Quantitative Real-Time PCR Methods for Measuring MicroRNA Levels in MSC Cultures
Cynthia Camarillo, Mavis Swerdel, and Ronald P. Hart

31. Two Dimensional Gel Electrophoresis Analysis of Mesenchymal Stem Cells
Monique Provansal, Christian Jorgensen, Sylvain Lehmann, Stéphane Roche

32. Proteomic Analysis of Human Mesenchymal Stem Cells
Guo Li, Chu-yan Chan, Hua Wang and Hsiang-fu Kung

33. Metabolic Labeling and Click Chemistry Detection of Glycoprotein Markers of Mesenchymal Stem Cell Differentiation
Courtenay Hart, Lucas G. Chase, Mahbod Hajivandi and Brian Agnew

34. BacMam-Mediated Gene Delivery Into Multipotent Mesenchymal Stromal Cells
Michael O'Grady, Robert H. Batchelor, Kelly Scheyhing, Christopher W. Kemp, George T. Hanson and Uma Lakshmipathy

35. Cell Surface Engineering of Mesenchymal Stem Cells
Debanjan Sarkar, Weian Zhao, Ashish Gupta, Wei Li Loh, Rohit Karnik, Jeffrey M. Karp


Titel: Mesenchymal Stem Cell Assays and Applications
EAN: 9781493957798
ISBN: 1493957791
Format: Kartonierter Einband
Herausgeber: Humana Press
Anzahl Seiten: 552
Gewicht: 1025g
Größe: H254mm x B178mm x T29mm
Jahr: 2017
Auflage: Softcover reprint of the original 1st ed. 2011

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