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Membrane Protein Structure Determination

  • Kartonierter Einband
  • 472 Seiten
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Membrane proteins, representing nearly 40% of all proteins, are key components of cells involved in many cellular processes, yet o... Weiterlesen
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Membrane proteins, representing nearly 40% of all proteins, are key components of cells involved in many cellular processes, yet only a small number of their structures have been determined. Membrane Protein Structure Determination: Methods and Protocols presents many detailed techniques for membrane protein structure determination used today by bringing together contributions from top experts in the field. Divided into five convenient sections, the book covers various strategies to purify membrane proteins, approaches to get three dimensional crystals and solve the structure by x-ray diffraction, possibilities to gain structural information for a membrane protein using electron microscopy observations, recent advances in nuclear magnetic resonance (NMR), and molecular modelling strategies that can be used either to get membrane protein structures or to move from atomic structure to a dynamic understanding of a molecular functioning mechanism. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and easy to use, Membrane Protein Structure Determination: Methods and Protocols serves as an ideal reference for scientists seeking to further our knowledge of these vital and versatile proteins as well as our overall understanding of the complicated world of cell biology.

Addresses significant hurdles to membrane protein purification

Describes techniques that permit the capture of atomic scale pictures of membrane proteins

Includes tips for avoiding protocol pitfalls

Part I: Membrane Protein Purification 1. Characterization of Membrane Protein Preparations: Measurement of Detergent Content and Ligand Binding after Proteoliposomes Reconstitution Mariano A. Ostuni, Soria Iatmanen, David Teboul, Jean-Claude Robert, and Jean-Jacques Lacapère 2. Native Membrane Proteins Versus Yeast Recombinant: An Example, the Mitochondrial ADP/ATP Carrier Bertrand Arnou, Cécile Dahout-Gonzalez, Ludovic Pelosi, Guy J.-M. Lauquin, Gérard Brandolin, and Véronique Trézéguet 3. Bacterial Overexpressed Membrane Proteins: An Example, the TSPO Jean-Claude Robert and Jean-Jacques Lacapère 4. Insect Cell Versus Bacterial Overexpressed Membrane Proteins: An Example, the Human ABCG2 Transporter Alexandre Pozza, José M. Perez-Victoria, and Attilio Di Pietro Part II: X-Ray Crystallography 5. Crystallography of Membrane Proteins: From Crystallization to Structure Aurélien Deniaud, Ekaterina Moiseeva, Valentin Gordeliy, and Eva Pebay-Peyroula 6. Structural Approaches of the Mitochondrial Carrier Family Hughes Nury, Iulia Blesneac, Stephanie Ravaud, and Eva Pebay-Peyroula 7. What Can Be Learned about the Function of a Single Protein from Its Various X-Ray Structures: The Example of the Sarcoplasmic Calcium Pump Jesper Vuust Møller, Claus Olesen, Anne-Marie Lund Winther, and Poul Nissen 8. Toward Drug Design: Recent Progress in the Structure of GPCR, a Membrane Protein with High Potential as a Pharmaceutical Target Vadim Cherezov, Enrique Abola, and Raymond C. Stevens Part III: Electron Microscopy 9. Observation of Membrane Proteins in situ: AQPcic, the Insect Aquaporin Example Daniel Thomas and Annie Cavalier 10. Two-Dimensional Crystallization of Integral Membrane Proteins for Electron Crystallography David L. Stokes, William J. Rice, Minghui Hu, Changki Kim, and Iban Ubarretxena 11. Structure Determination of Membrane Protein by Both Cryo Electron Tomography and Single Particle Analysis Sylvain Trépout, Jean-Christophe Taveau,and Olivier Lambert 12. Electron Microscope Tomography of Native Membranes Gabriel Péranzi, Cedric Messaoudi, Leeyah Issop, and Jean-Jacques Lacapère 13. From Electron Microscopy Maps to Atomic Structures Using Normal Mode-Based Fitting Konrad Hinsen, Edward Beaumont, Bertrand Fournier, and Jean-Jacques Lacapère Part IV: Nuclear Magnetic Resonance 14. Determination of Membrane Protein Structures Using Solution and Solid-State NMR Pierre Montaville and Nadège Jamin 15. Membrane Protein Fragments Reveal Both Secondary and Tertiary Structure of Membrane Proteins Philip L. Yeagle and Arlene D. Albert 16. What Can We Learn From a Small Regulatory Membrane Protein? Gianluigi Veglia, Kim N. Ha, Lei Shi, Raffaello Verardi, and Nathaniel J. Traaseth 17. Solution-State NMR Spectroscopy for Investigating 3D Structure of Membrane Proteins in Detergent Micelles: Structure of the Klebsiella pneumoniae Outer Membrane Protein, KpOmpA Marie Renault, Olivier Saurel, Pascal Demange, Virginie Reat, and Alain Milon 18. NMR Spectroscopy of Lipid Bilayers Axelle Grélard, Cécile Loudet, Anna Diller, and Erick J. Dufourc Part V: Molecular Modelling 19. Critical Review of General Guidelines for Membrane Proteins Model Building and Analysis Catherine Etchebest and Gaelle Debret 20. 3D-Structural Models of Transmembrane Proteins Alexandre G. de Brevern 21. Molecular Dynamics of Membrane Peptides and Proteins: Principles and Comparison to Experimental Data Patrick F.J. Fuchs 22. Membrane Protein Dynamics from Femtoseconds to Seconds Christian Kandt and Luca Monticelli 23. The Family of G Protein-Coupled Receptors: An Example of Membrane Proteins Irina G. Tikhonova and Daniel Fourmy


Titel: Membrane Protein Structure Determination
Untertitel: Methods and Protocols
EAN: 9781493961177
ISBN: 1493961179
Format: Kartonierter Einband
Herausgeber: Humana Press
Anzahl Seiten: 472
Gewicht: 880g
Größe: H254mm x B178mm x T25mm
Jahr: 2016
Auflage: Softcover reprint of the original 1st ed. 2010

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