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In this present volume, different approaches are detailed to produce membrane proteins, purify them, study their function, determine their structure, and model them in membrane. Since every membrane protein behaves mostly in a unique way /fashion, knowledge of guidelines and tricks may help to increase chances to express, purify and characterize a peculiar membrane protein. Production of correctly folded protein remains a challenge. Moreover, getting a functional and stable protein requires to optimize membrane mimicking environments that can be detergent or artificial membranes. In some cases, the finding of the correct ligand which will stabilize the desired conformation is needed. In other cases, stabilization can be obtained using specific antibodies. This volume also presents different techniques to analyze the functional status of membrane proteins. Written in the highly successful Methods in Molecular Biology series format, chapters in Membrane Protein Structure and Function Characterization: Methods and Protocols provide **different techniques to analyze the functional and structural status of membrane proteins. Chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Membrane Protein Structure and Function Characterization: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Includes cutting-edge methods and protocols
Provides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Includes supplementary material: sn.pub/extras
Includes supplementary material: sn.pub/extras
Inhalt
Recombinant Overexpression of Mammalian TSPO Isoforms 1 and 2 Lucile Senicourt, Soria Iatmanen-Harbi, Claude Hattab, Mariano A Ostuni, Marie-France Giraud, and Jean-Jacques Lacapere
Functional Assembly of Soluble and Membrane Recombinant Proteins of Mammalian NADPH Oxidase Complex
Hajer Souabni, Aymen Ezzine, Tania Bizouarn, and Laura Baciou
Lucie Hartmann, Estelle Metzger, Noémie Ottelard, and Renaud Wagner
Isabelle Larieu, James Tolchard, Corinne Sanchez, Edmond Yazo Kone, Alexandre Barras, Claire Stines-Chaumeil, Benoit Odaert, and Marie-France Giraud
Marie-Laure Faugeron, Aurelie Badillo, Francois Penin, and Anja Bocjmann
Vilius Kurauskas, Paul Schanda, and Remy Sounier
Francesca Gubellini and Rémi Fronzes
Ghérici Hassaine, Cédric Deluz, Luigino Grasso, Romain Wyss, Ruud Hovius, Henning Stahlberg, Takashi Tomizaki, Aline Desmyter, Christophe Moreau, Lucie Peclinovska, Sonja Minniberger, Lamia Mebarki, Xiao-Dan Li, Horst Vogel, and Hugues Nury
Francoise Bonnete and Patrick J Loll
Batoul Srour, Stefan Bruechert, Susana Andrade, and Petra Hellwig
Jeroen F Van Dyck, Albert Konijnenberg, and Frank Sobott
Luc NEGRONI, Michel ZIVY, and Claire LEMAIRE
Yashvin Neehaul, Sebastien Kriegel, Blanca Barquera, and Petra Hellwig
Alice Verchere, Isabelle Broutin, and Martin Picard
Michel Vivaudou, Zlatomir Todorov, Gina Catalina Reyes-Mejia, and Christophe Moreau
Philippe Rosier, Frederic Gelebart, Nicolas Dumesnil, Gauthier Esnot, Manuela Dezi, Marc Morand, and Catherine Venien-Bryan
Laetitia Daury, Jean-Christophe Taveau, Dimitri Salvador, Marie Glavier, and Olivier Lambert
Lucile Senicourt, Luminita Duma, Vassilios Papadopoulos, and Jean-Jacques Lacapere
Denis Lacabanne, Britta Kunert, Carole Gardiennet, Beat H Meier, and Anja Bockmann
Samuel Murail
Aurore Vaitinadapoule and Catherine Etchebest