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MAP Kinase Signaling Protocols

  • Kartonierter Einband
  • 544 Seiten
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Exploring the activation and function of components of the MAPK signaling cascades, this volume presents fully updated techniques ... Weiterlesen
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Exploring the activation and function of components of the MAPK signaling cascades, this volume presents fully updated techniques to study MAPK. Chapters include lists of the necessary materials, step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Mitogen-activated protein (MAP) kinase (MAPK) cascades are key signaling components that govern essentially all cellular processes evoked by any type of stimulation, and it has been well established that the malfunctioning of these cascades leads to various diseases including cancer, autoimmunity, and diabetes. In MAP Kinase Signaling Protocols, Second Edition, expert researchers fully update the popular first edition the key techniques used in the study of MAPK signaling cascades in various cellular contexts. This thorough volume explores essential topics such as activation and function of components of the MAPK signaling cascades, the study of MAPK cascades as transmitters of membranal receptor signals, structure-function relationships of MAPKs, studies on the regulation of MAPK cascades, the use of lower organisms, animal models, and human genetics in the study of MAPKs, as well as the study of MAPKs in specific systems and diseases. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters includes introductions to their respective subjects, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, MAP Kinase Signaling Protocols, Second Edition aims to facilitate the study of MAPKs and allow for quicker progress in our knowledge of many vital cellular processes as well as devastating diseases.


Part I: Activation and Function of Components of the MAP Kinase Signaling Cascades

1. The MAP Kinase Signaling Cascades: A System of Hundreds of Components Regulates a Diverse Array of Physiological Functions
Yonat Keshet and Rony Seger

2. Determination of ERK Activity: Anti-Phospho-ERK Antibodies and In vitro Phosphorylation
Shiri Procaccia, Sarah Kraus, and Rony Seger

3. Activation of SAPK/JNKs In vitro
Deborah N. Chadee and John M. Kyriakis

4. Activation of p38 and Determination of Its Activity
Huamin Zhou, Jianming Chen, and Jiahuai Han

5. Activity Assays for Extracellular Signal-Regulated Kinase 5
Kazuhiro Nakamura and Gary L. Johnson

6. Use of Inhibitors in the Study of MAP Kinases
Kimberly Burkhard and Paul Shapiro

Part II: Study of MAP Kinase Cascades as Transmitters of Membranal Receptor Signals

7. MAP Kinase Activation by Receptor Tyrosine Kinases: In Control of Cell Migration
Gabi Tarcic and Yosef Yarden

8. Activation of Ras and Rho GTPases and MAP Kinases by G Protein-Coupled Receptors
Mario Chiariello, Jose P. Vaqué, Piero Crespo, and J. Silvio Gutkind

9. Regulation of MAP Kinase Signaling by Calcium
Colin D. White and David B. Sacks

10. Identification of Novel Substrates of MAP Kinase Cascades Using Bioengineered Kinases that Uniquely Utilize Analogs of ATP to Phosphorylate Substrates
Hui Zheng, Adnan Al-Ayoubi, and Scott T. Eblen

11. ERK-MAP Kinase Signaling in the Cytoplasm
Michelle C. Mendoza, Ekrem Emrah Er, and John Blenis

12. Lentiviral Vectors to Study the Differential Function of ERK1 and ERK2 MAP Kinases
Marzia Indrigo, Alessandro Papale, Daniel Orellana and Riccardo Brambilla

Part III: Structure-Function Relationships and Localization of MAP Kinases

13. Structural Studies of MAP Kinase Cascade Components
Elizabeth J. Goldsmith, Xiaoshan Min, Haixia He, and Tianjun Zhou

14. Analysis of MAP Kinases by Hydrogen Exchange Mass Spectrometry
Kevin M. Sours and Natalie G. Ahn

15. A 'Molecular Evolution' Approachfor Isolation of Intrinsically Active (MEK-Independent) MAP Kinases
Vered Levin-Salomon, Oded Livnah, and David Engelberg

16. Reconstitution of the Nuclear Transport of the MAP Kinase ERK2
Arif Jivan, Aarati Ranganathan, and Melanie H. Cobb

17. Localization and Trafficking of Fluorescently Tagged ERK1 and ERK2
Matilde Marchi, Riccardo Parra, Mario Costa, and Gian Michele Ratto

Part IV: Studies on the Regulation of MAP Kinase Cascades

18. Studying the Regulation of MAP Kinase by MAP Kinase Phosphatases in vitro and in Cell Systems
Céline Tárrega, Caroline Nunes-Xavier, Rocío Cejudo-Marín, Jorge Martín-Pérez, and Rafael Pulido

19. Proteomic Analysis of Scaffold Proteins in the ERK Cascade
Melissa M. McKay and Deborah K. Morrison

20. Analysis of ERKs' Dimerization by Electrophoresis
Adán Pinto and Piero Crespo

21. MAP Kinase - SUMO Pathway Interactions
Shen-Hsi Yang and Andrew D. Sharrocks

22. Computational Modelling of Kinase Signalling Cascades
David Gilbert, Monika Heiner, and Rainer Breitling

Part V: Use of Lower Organisms, Animal Models, and Human Genetics in the Study of MAP Kinases

23. Analysis of MAP Kinase Activity in Yeast
Elaine A. Elion and Rupam Sahoo

24. Detection of RTK Pathway Activation in Drosophila Using Anti-dpERK Immunofluorescence Staining
Aharon Helman and Ze'ev Paroush

25. Studying MAP Kinase Pathways During Early Development of Xenopus laevis
Aviad Keren and Eyal Bengal

26. Deciphering Signaling Pathways In vivo: The Ras/Raf/Mek/Erk Cascade
Gergana Galabova-Kovacs and Manuela Baccarini

27. Mutational and Functional Analysis in Human Ras/MAP Kinase Genetic Syndromes
William E. Tidyman and Katherine A. Rauen

Part VI: Study of MAP Kinases in Specific Physiological Systems

28. Implication of the ERK Pathway on the Post Transcriptional Regulation of VEGF mRNA Stability
Khadija Essafi-Benkhadir, Jacques Pouysségur, and Gilles Pagès

29. Studies on MAP Kinase Signaling in the Immune System
Hongbo Chi and


Titel: MAP Kinase Signaling Protocols
EAN: 9781493961054
ISBN: 1493961055
Format: Kartonierter Einband
Herausgeber: Humana Press
Anzahl Seiten: 544
Gewicht: 1011g
Größe: H254mm x B178mm x T29mm
Jahr: 2016
Auflage: Softcover reprint of the original 2nd ed. 2010

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