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Mammalian Cell Viability

  • Fester Einband
  • 252 Seiten
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This book presents methods for monolayer and suspension cells, methods for determining viability within tissue sections and 3 dimensional culture systems, and methods requiring specialized equipment, all in the popular Methods in Molecular Biology(TM) series format.

Whether the question is one of basic cell survival, or whether it is being used to correlate cell number to some other factor such as matrix synthesis, an estimate of cell viability is universally required. In Mammalian Cell Viability: Methods and Protocols, experts in the field describe methods from the most basic which can be performed in any laboratory, to some which require specific pieces of equipment. Initially focusing on methods for monolayer and suspension cells, later chapters describe methods for determining viability within tissue sections and 3 dimensional culture systems. Finally, methods requiring highly specialized equipment are described in order to explain what is possible. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting and avoiding known pitfalls.

Practical and adaptable, Mammalian Cell Viability: Methods and Protocols serves as a self-contained laboratory manual useful to both experienced researchers and those new to this incredibly important and influential field.

Features protocols utilizing both common lab equipment and specialized, cutting-edge equipment

Facilitates the multiplexing of methods for the greatest levels of success

Contains expert tips and key implementation advice


1. Cell Viability Assays: Introduction

Martin J. Stoddart

2. Cell Viability Analysis Using Trypan Blue: Manual and Automated Methods

Kristine S. Louis and Andre C. Siegel

3. Estimation of Cell Number Based on Metabolic Activity: The MTT Reduction Assay

László Kupcsik

4. WST8 Analysis of Cell Viability During Osteogenesis of Human Mesenchymal Stem Cells

Martin J. Stoddart

5. Assessment of Cell Proliferation with Resazurin-Based Fluorescent Dye

Ewa M. Czekanska

6. The xCELLigence System for Real-Time and Label-Free Monitoring of Cell Viability

Ning Ke, Xiaobo Wang, Xiao Xu, and Yama A. Abassi

7. Analysis of Tumor and Endothelial Cell Viability and Survival Using Sulforhodamine B (SRB) and Clonogenic Assays

Caroline Woolston and Stewart Martin

8. Annexin V / 7-AAD Staining in Keratinocytes

Maya Zimmermann and Norbert Meyer

9. Measurement of Caspase Activity: From Cell Populations to Individual Cells

Gabriela Paroni and Claudio Brancolini

10. Rapid Quantification of Cell Viability and Apoptosis in B-Cell Lymphoma Cultures Using Cyanine SYTO Probes

Donald Wlodkowic, Joanna Skommer, and Zbigniew Darzynkiewicz

11. Multiplexing Cell Viability Assays

Helga H.J. Gerets, Stephane Dhalluin, and Franck A. Atienzar

12. Cytotoxicity Testing: Measuring Viable Cells, Dead Cells, and Detecting Mechanism of Cell Death

Terry L. Riss, Richard A. Moravec, and Andrew L. Niles

13. Fluorescein Diacetate for Determination of Cell Viability in 3D Fibroblast-Collagen-GAG Constructs

Heather M. Powell, Alexis D. Armour, and Steven T. Boyce

14. Confocal Imaging Protocols for Live/Dead Staining in 3-Dimensional Carriers

Benjamin Gantenbein-Ritter, Christoph M. Sprecher, Samantha Chan, Svenja Illien-Jünger, and Sibylle Grad

15. Viability Assessment of Osteocytes Using Histological Lactate Dehydrogenase Activity Staining on Human Cancellous Bone Sections

Katharina Jähn and Martin J. Stoddart

16. Measuring Glutamate Receptor Activation-Induced Apoptotic Cell Death in Ischemic Rat Retina Using the TUNEL Assay

Won-Kyu Ju and Keun-Young Kim

17. Exploiting the Liberation of Zn2+ to Measure Cell Viability

Christian J. Stork and Yang V. Li

18. Non-Invasive Bioluminescent Quantification of Viable Stem Cells in Engineered Constructs

Karim Oudina, Adeline Cambon-Binder, and Delphine Logeart-Avramoglou

19. Raman Micro-Spectroscopy as a Non-Invasive Cell Viability Test

Sophie Verrier, Alina Zoladek, and Ioan Notingher

20. Closed Ampoule Isothermal Microcalorimetry (IMC) for Continuous Real-Time Detection and Evaluation of Cultured Mammalian Cell Activity and Responses

Olivier Braissant and Alma U. Dan Daniels

21. Digital Image Processing of Live/Dead Staining

Pieter Spaepen, Sebastian De Boodt, Jean-Marie Aerts, and Jos Vander Sloten


Titel: Mammalian Cell Viability
Untertitel: Methods and Protocols
EAN: 9781617791079
ISBN: 1617791075
Format: Fester Einband
Herausgeber: Humana Press
Anzahl Seiten: 252
Gewicht: 678g
Größe: H260mm x B183mm x T17mm
Jahr: 2011
Untertitel: Englisch
Auflage: 2011

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