Mammalian Cell Viability

This book presents methods for monolayer and suspension cells, methods for determining viability within tissue sections and 3 dimensional culture systems, and methods requiring specialized equipment, all in the popular Methods in Molecular Biology(TM) series format.

Whether the question is one of basic cell survival, or whether it is being used to correlate cell number to some other factor such as matrix synthesis, an estimate of cell viability is universally required. In Mammalian Cell Viability: Methods and Protocols , experts in the field describe methods from the most basic which can be performed in any laboratory, to some which require specific pieces of equipment. Initially focusing on methods for monolayer and suspension cells, later chapters describe methods for determining viability within tissue sections and 3 dimensional culture systems. Finally, methods requiring highly specialized equipment are described in order to explain what is possible. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting and avoiding known pitfalls.

Practical and adaptable, Mammalian Cell Viability: Methods and Protocols serves as a self-contained laboratory manual useful to both experienced researchers and those new to this incredibly important and influential field.

Features protocols utilizing both common lab equipment and specialized, cutting-edge equipment Facilitates the multiplexing of methods for the greatest levels of success Contains expert tips and key implementation advice

Inhalt

Cell Viability Assays: Introduction.- Cell Viability Analysis Using Trypan Blue: Manual and Automated Methods.- Estimation of Cell Number Based on Metabolic Activity: The MTT Reduction Assay.- WST8 Analysis of Cell Viability During Osteogenesis of Human Mesenchymal Stem Cells.- Assessment of Cell Proliferation with Resazurin-Based Fluorescent Dye.- The xCELLigence System for Real-Time and Label-Free Monitoring of Cell Viability.- Analysis of Tumor and Endothelial Cell Viability and Survival Using Sulforhodamine B (SRB) and Clonogenic Assays.- Annexin V / 7-AAD Staining in Keratinocytes.- Measurement of Caspase Activity: From Cell Populations to Individual Cells.- Rapid Quantification of Cell Viability and Apoptosis in B-Cell Lymphoma Cultures Using Cyanine SYTO Probes.- Multiplexing Cell Viability Assays.- Cytotoxicity Testing: Measuring Viable Cells, Dead Cells, and Detecting Mechanism of Cell Death.- Fluorescein Diacetate for Determination of Cell Viability in 3D Fibroblast-Collagen-GAG Constructs.- Confocal Imaging Protocols for Live/Dead Staining in 3-Dimensional Carriers.- Viability Assessment of Osteocytes Using Histological Lactate Dehydrogenase Activity Staining on Human Cancellous Bone Sections.- Measuring Glutamate Receptor Activation-Induced Apoptotic Cell Death in Ischemic Rat Retina Using the TUNEL Assay.- Exploiting the Liberation of Zn 2+ to Measure Cell Viability.- Non-Invasive Bioluminescent Quantification of Viable Stem Cells in Engineered Constructs.- Raman Micro-Spectroscopy as a Non-Invasive Cell Viability Test.- Closed Ampoule Isothermal Microcalorimetry (IMC) for Continuous Real-Time Detection and Evaluation of Cultured Mammalian Cell Activity and Responses.-Digital Image Processing of Live/Dead Staining.