Macro-Glycoligands

This volume details methods and protocols for the synthesis and characterization of glycopolymers and their biomedical applications. Chapters are divided into three parts covering synthesis and characterization of glycopolymers; glycopolymer-nanoparticle conjugates; and surface immobilized glycopolymers. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Macro-Glycoligands: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt

Synthesis of Chain-end Functionalized Glycopolymers via Cyanoxyl Mediated Free Radical Polymerization (CMFRP).- Protecting-group-free Synthesis of Well-defined Glycopolymers Featuring Negatively Charged Oligosaccharides.- Glycopolymers Prepared by Ring-opening Metathesis Polymerization Followed by Glycoconjugation Using a Triazole Forming 'Click' Reaction.- Protecting-group-free Synthesis of Glycopolymers and Their Binding Assay with Lectin and Influenza Virus.- Carbohydrate-based Initiators for the Cationic Ring-opening Polymerization of 2-Ethyl-2-oxazoline.- Heterofunctional Glycopolypeptides by Combination of Thiol-ene Chemistry and NCA Polymerization.- Preparation of Proteoglycan Mimetic Graft Copolymers.- Galactosylated Polymer Nano-objects by Polymerization-Induced Self-Assembly, Potential Drug Nanocarriers.- Synthetic Approach to Biotinylated Glyco-Functionalized Quantum Dots: A New Fluorescent Probes for Biomedical Applications.- Surface Modification of Polydivinylbenzene Microspheres with a Fluorinated Glycopolymer using Thiol-Halogen Click Chemistry.- Glycopolymer-grafted Polymer Particles for Lectin Recognition.- Synthesis of Non-spherical Glycopolymer-decorated Nanoparticles: Combing Thiol-ene with Catecholic Chemistry.- Synthetic Approach To Glycopolymer Base Nanoparticle Gold(I) Conjugate: A New Generation Of Therapeutic Agents.- Multivalent Glycopolymer-coated Gold Nanoparticles.- Modulation of Multivalent Protein Binding on Surfaces by Glycopolymer Brush Chemistry.- Oriented ImmobilizedSialyloligo-Macroligand Microarray.- Glycocalyx Remodeling with Glycopolymer-based Proteoglycan Mimetics.