Long Non-Coding RNAs

This volume assembles a broad spectrum of methods used in long non-coding RNAs (lncRNA) research, ranging from computational annotation of lncRNA genes to molecular and cellular analyses of the function of individual lncRNA. Long Non-Coding RNAs: Methods and Protocols also discusses methods used to study circular RNAs and RNA splicing, as well as influential findings on lncRNA in human diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Thorough and cutting-edge, Long Non-Coding RNAs: Methods and Protocols is a must-have for molecular biologists, cell and developmental biologists, specialists who conduct disease-oriented research, and bioinformatics experts who seek a better understanding on lncRNA expression and function by computational analysis of the massive sequencing data that are rapidly accumulating in recent years.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras

Autorentext

Lin Zhang, MD Center for Research on Reproduction and Women's Health University of Pennsylvania School of Medicine BRB II/III, 421 Curie Blvd. Rm 1324-1326 and 1343 (Lab), Rm 1209 (Office) Philadelphia, PA 19104 Yi Feng Department of Cancer Biology University of Pennsylvania School of Medicine Rm 625 BRB II/III, 421 Curie Blvd. Philadelphia, PA 19104

Inhalt

LncRNA Pulldown Combined with Mass Spectrometry to Identify the Novel LncRNA-Associated Proteins.- Cross-Linking Immunoprecipitation and qPCR (CLIP-qPCR) Analysis to Map Interactions between Long Non-Coding RNAs and RNA-Binding Proteins.- Characterization of Long Non-Coding RNA Associated Proteins by RNA-Immunoprecipitation.- Isolation of Protein Complexes Associated with Long Non-Coding RNAs.- Profiling Long Non-Coding RNA Expression Using Custom-Designed Microarray.- Long Non-Coding RNA Expression Profiling Using Arraystar LncRNA Microarrays.- Nuclear RNA Isolation and Sequencing.- Targeted LncRNA Sequencing with the SeqCap RNA Enrichment System.- ChIP-Seq: Library Preparation and Sequencing.- S tellaris ® RNA Fluorescence In Situ Hybridization for the Simultaneous detection of Immature and Mature Long Non-Coding RNAs in Adherent Cells.- Simultaneous RNA-DNA FISH.- Visualizing Long Non-Coding RNAs on Chromatin.- Non-Isotopic Method for In Situ LncRNA Visualization and Quantitation.- Detection of Long Non-Coding RNA Expression by Non-Radioactive Northern Blots.- Assessment of In Vitro SiRNA Delivery in Cancer Mouse Models.- Targeting Long Non-Coding RNA with Antisense Oligonucleotide Technology as Cancer Therapeutics.- Characterization of Circular RNAs.- Methods for Characterization of Alternative RNA Splicing.- NONCODEv4: Annotation of Non-Coding RNAs with Emphasis on Long Non-Coding RNAs.- Computational Analysis of LncRNA from cDNA Sequences.- AnalyzingMiRNA: LncRNA Interactions.- Long Non-Coding RNAs: An Overview.

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