Lipidomics

This guide on methods and protocols of lipidomics covers a wide array of topics including isolation techniques, structural analysis, lipid rafts, lipid trafficking and profiling, biomarkers, lipid peroxidation, software tools and bioinformatics.

Essential in biological functions like cell signaling and, when disturbed, a likely cause of disease, lipids have proven to be a vital force in cell biology. In Lipidomics: Methods and Protocols , an international panel of experts present a wide variety of reviewed as well as unpublished data on isolation techniques, structural analysis, lipid rafts, lipid trafficking and profiling, biomarkers, lipid peroxidation, biostatistics applied to lipids, software tools, and bioinformatics. These studies range from simple systems, such as in yeast, to complex biological models. Samples used in these studies include: mitochondria, microsomes, endosomal membranes, RBS and plasma membranes, vesicles, caveolae, liposomes, macrophages, meibum, brain, microglia, retina, adipose tissue, keratinocytes, pulmonary surfactant, and hepatic cultures. Volume 1 focuses on shotgun and global lipidomics, analytical approaches, and lipid maps. Written in the highly successful Methods in Molecular Biology ™ series format, the chapters include useful introductions to their respective topics, lists of the necessary equipment and materials, step-by-step, readily reproducible laboratory protocols, and authoritative notes on troubleshooting and avoiding common pitfalls.

Comprehensive and cutting-edge, Lipidomics: Methods and Protocols will support researchers who wish to increase their utilization of lipidomics, which will certainly lead to more powerful technology, improved diagnostic-prognostic capabilities, and the identification of new valuable products.

Regarding Volume 1 and 2: Contains in-depth guides to isolation and processing of lipid samples, probes, lipid profiling, signaling, transport, and mapping Provides examples utilizing key model systems Presents oxidized lipids obtained from in vitro and in vivo biological material, redox platforms, high throughput systems, microscopy and imaging, and computer simulation Serves as a comprehensive guide to technical applications Explores bioinformatics strategy and statistical models for standardization of lipids Includes supplementary material: sn.pub/extras

Inhalt
Shotgun and Global Lipidomics.- Examination of the Brain Mitochondrial Lipidome Using Shotgun Lipidomics.- Tracking the Glycerophospholipid Distribution of Docosahexaenoic Acid by Shotgun Lipidomics.- Global Analysis of Retina Lipids by Complementary Precursor Ion and Neutral Loss Mode Tandem Mass Spectrometry.- Combining Lipidomics and Proteomics of Human Cerebrospinal Fluids.- Analytical Approaches.- Lipid Profiling Using Two-Dimensional Heteronuclear Single Quantum Coherence NMR.- Capabilities and Drawbacks of Phospholipid Analysis by MALDI-TOF Mass Spectrometry.- Lipidomics of the Red Cell in Diagnosis of Human Disorders.- HPLC/MS/MS-Based Approaches for Detection and Quantification of Eicosanoids.- Brain Phosphoinositide Extraction, Fractionation, and Analysis by MALDI-TOF MS.- Lipidomic Analysis of Biological Samples by Liquid Chromatography Coupled to Mass Spectrometry.- Lipidomic Analysis of Human Meibum Using HPLCMSn.- Lipid Geographical Analysis of the Primate Macula by Imaging Mass Spectrometry.- A Novel Role for Nutrition in the Alteration of Functional Microdomains on the Cell Surface.- Lipidomic Analysis of Prostanoids by Liquid ChromatographyElectrospray Tandem Mass Spectrometry.- Qualitative and Quantitative Analyses of Phospholipids by LCMS for Lipidomics.- Determination of Fatty Acid Profiles and TAGs in Vegetable Oils by MALDI-TOF/MS Fingerprinting.- Dynamics of Adipose Tissue Development by 2H2O Labeling.- Analysis of Lipid Particles from Yeast.- Mammalian Fatty Acid Elongases.- Membrane Lipidomics and the Geometry of Unsaturated Fatty Acids From Biomimetic Models to Biological Consequences.- OnLine Ozonolysis Methods for the Determination of Double Bond Position in Unsaturated Lipids.- Comprehensive Quantitative Analysis of BioactiveSphingolipids by High-Performance Liquid ChromatographyTandem Mass Spectrometry.- The Use of Charged Aerosol Detection with HPLC for the Measurement of Lipids.- Lipid Maps.- Non-invasive Mapping of Lipids in Plant Tissue Using Magnetic Resonance Imaging.- Mapping the Lipolytic Proteome of Adipose Tissue Using Fluorescent Suicide Inhibitors.- Identifying the Spatial Distribution of Vitamin E, Pulmonary Surfactant and Membrane Lipids in Cells and Tissue by Confocal Raman Microscopy.