Laser Capture Microdissection

This second edition provides practical advice on tissue-based laser microdissection using different laser systems. Readers will find detailed descriptions of the individual laser based micro-dissection systems in each chapter.

Laser microdissection techniques have revolutionized the ability of researchers in general, and pathologists in particular, to carry out molecular analysis on specific types of normal and diseased cells and to fully utilize the power of current molecular technologies including PCR, microarrays, and proteomics. In second edition of Laser Capture Microdissection: Methods and Protocols , experts in the field provide the reader with practical advice on how to carry out tissue-based laser microdissection successfully in their own laboratory using the different laser microdissection systems that are available and to apply a wide range of molecular technologies. The individual chapters encompass detailed descriptions of the individual laser based micro-dissection systems. The downstream applications of the laser microdissected tissue described in the book include PCR in its many different forms as well as gene expression analysis including application to microarrays and proteomics. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Laser Capture Microdissection: Methods and Protocols, Second Edition is an ideal resource for researchers striving to move forward our understanding of normal physiology and pathology.

Provides practical methods and protocols involving tissue-based laser microdissection, ideal for labs around the globe Fully updates and reexamines the popular first edition Features key notes and expert implementation advice Includes supplementary material: sn.pub/extras

Inhalt

Laser Capture Microdissection: Methods and Applications.- Laser Microdissection for Gene Expression Profiling.- Gene Expression Using the PALM System.- Immuno-Guided Microdissection Techniques.- Optimized RNA Extraction from Non-Deparaffinized, Laser-Microdissected Material.- Laser Capture Microdissection for Analysis of Gene Expression in Formalin-Fixed Paraffin-Embedded Tissue.- MicroRNA Profiling Using RNA from Microdissected Immunostained Tissue.- Profiling Solid Tumor Heterogeneity by LCM and Biological MS of Fresh Frozen Tissue Sections.- Amplification Testing in Breast Cancer by Multiplex Ligation-Dependent Probe Amplification of Microdissected Tissue.- Detection and Quantification of MicroRNAs in Laser Microdissected Formalin-Fixed Paraffin-Embedded Breast Cancer Tissues.- Laser Capture Microdissection Applications in Breast Cancer Proteomics.- Proteomic Analysis of Laser Microdissected Ovarian Cancer Tissue with SELDI-TOF MS.- LCM Assisted Biomarker Discovery from Archival Neoplastic Gastrointestinal Tissues.- Purification of Diseased Cells from Barrett's Esophagus and Related Lesions by Laser Capture Microdissection.- Laser Microdissection of Intestinal Epithelial Cells and Downstream Analysis.- Application of Laser Microdissection and Quantitative PCR to Assess the Response of Oesophageal Cancer to Neoadjuvant Chemo-Radiotherapy.- Oligonucleotide Microarray Expression Profiling of Contrasting Invasive Phenotypes in Colorectal Cancer.- Evaluation of Gastrointestinal mtDNA Depletion in Mitochondrial Neurogastrointestinal Encephalomyopathy (MNGIE).- Laser Microdissection for Gene Expression Study of Hepatocellular Carcinomas Arising in Cirrhotic and Non-Cirrhotic Livers.- Laser Capture Microdissection of Pancreatic Ductal Adeno-Carcinoma Cells to AnalyzeEzH2 by Western Blot Analysis.- Laser-Capture Microdissection of Renal Tubule Cells and Linear Amplification of RNA for Microarray Profiling and Real-Time PCR.- Subcellular Renal Proximal Tubular Mitochondrial Toxicity with Tenofovir Treatment.- Application of Laser Capture Microdissection to Study Renal Carcinogenesis.- Laser Capture Microdissection and Transcriptional Profiling in Archival FFPE Tissue in Prostate Cancer.- Quantitative Analysis of the Enzymes Associated with 5-Fluorouracil Metabolism in Prostate Cancer Biopsies.- Microdissection of Gonadal Tissues for Gene Expression Analyses.- Duplex Real Time PCR Assay for Quantifying Mitochondrial DNA Deletions in Laser Microdissected Single Spiral Ganglion Cells.- Neuronal Type-Specific Gene Expression Profiling and Laser-Capture Microdissection.- Region-Specific In Situ Hybridization-Guided Laser Capture Microdissection on Postmortem Human Brain Tissue Coupled with Gene Expression Quantification.- UV-Laser Microdissection and mRNA Expression Analysis of Individual Neurons from Post Mortem Parkinson's Disease Brains.- Transcriptome Profiling of Murine Spinal Neurulation Using Laser Capture Microdissection and High-Density Oligonucleotide Microarrays.- Probing the CNS Microvascular Endothelium by Immune-Guided Laser Capture Microdissection Coupled to Quantitative RT-PCR.- Laser Capture Microdissection for Factor VIII-Expressing Endothelial Cells in Cancer Tissues.- Laser Capture Microdissection and Analysis of Liver Endothelial Cells from Patients with Budd Chiari Syndrome.- Laser Capture Microdissection of Hyperlipidemic/ApoE -/- Mouse Aorta Atherosclerosis.- Gene Expression Profiling in Laser Microdissected Bone Marrow Megakaryocytes.- Specific RNA Collection from the Rat Endolymphatic Sac by LaserCapture Microdissection (LCM): LCM of Very Small Organ Surrounded by Bony Tissue.- The Use of Laser Capture Microdissection on Adult Human Articular Cartilage for Gene Expression Analysis.- Laser Capture Microdissection of Developing Barley Seeds and cDNA Array Analysis of Selected Tissues.- Quantitative RT-PCR Gene Expression Analysis of a Laser Microdissected Placenta: An Approach to Study Preeclampsia.