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Kinase Screening and Profiling

  • Kartonierter Einband
  • 236 Seiten
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This volume focuses on screening and profiling kinase inhibitors or activators. Chapters in this book cover several facets of the ... Weiterlesen
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This volume focuses on screening and profiling kinase inhibitors or activators. Chapters in this book cover several facets of the drug discovery processes starting with target identification, and assay development and screening chemical libraries for hit identification and lead optimization. The profiling portion of Kinase Screening and Profiling: Methods and Protocols discusses several strategies and techniques that are required to minimize off target hits and reduce cellular toxicities that are caused by liability kinases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Timely and authoritative, Kinase Screening and Profiling: Methods and Protocols, will benefit scientists and researchers who are interested in exploring the role of kinases in academic, as well as pharmaceutical research.

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts


1. HTRF Kinase Assay Development and Methods in Inhibitor Characterization
Yong Jia, Mari Manuia, and Jose Juarez

2. Application of Eukaryotic Elongation Factor-2 Kinase (eEF-2K) for Cancer Therapy: Expression, Purification, and High-Throughput Inhibitor Screening
Clint D. J. Tavares, Ashwini K. Devkota, Kevin N. Dalby, and Eun Jeong Cho

3. Recombinant Kinase Production and Fragment Screening by NMR Spectroscopy
Byeonggu Han and Hee-Chul Ahn

4. Bioluminescence Methods for Assaying Kinases in Quantitative High-Throughput Screening (qHTS) Format Applied to Yes1 Tyrosine Kinase, Glucokinase and PI5P4K Lipid Kinase
Mindy I. Davis, Douglas S. Auld, and James Inglese

5. Using Bioluminescent Kinase Profiling Strips to Identify Kinase Inhibitor Selectivity and Promiscuity
Hicham Zegzouti, Jacquelyn Hennek, and Said A. Goueli

6. Measuring Activity of Phosphoinositide Lipid Kinases Using a Bioluminescent ADP-Detecting Assay
Andrew W. Tai and Jolanta Vidugiriene

7. A High-Throughput Radiometric Kinase Assay
Krisna C. Duong-Ly and Jeffrey R. Peterson

8. A High Content Assay to Screen for Modulators of EGFR Function
Christophe Antczak and Hakim Djaballah
9. Monitoring Protein Kinase Expression and Phosphorylation in Cell Lysates with Antibody Microarrays
Hong Zhang, Xiaoqing Shi, and Steven Pelech

10. From Enzyme to Whole Blood: Sequential Screening Procedure for Identification and Evaluation of p38 MAPK Inhibitors
Silke M. Bauer, Jakub M. Kubiak, Ulrich Rothbauer, and Stefan Laufer

11. Genetically Encoded Fluorescent Indicators to Visualize Protein Phosphorylation in Living Cells
Moritoshi Sato and Yoshio Umezawa

12. Characterization of an Engineered Src Kinase to Study Src Signaling and Biology
Leanna R. Gentry, Andrei V. Karginov, Klaus M. Hahn, and Channing J. Der

13. Screening One-Bead-One-Compound Peptide Libraries for Optimal Kinase Substrates
Thi B. Trinh and Dehua Pei

14. Determination of the Substrate Specificity of Protein Kinases with Peptide Micro- and Macroarrays
Shenshen Lai, Dirk F.H. Winkler, Hong Zhang, and Steven Pelech

15. Rapid Identification of Protein Kinase Phosphorylation Site Motifs Using Combinatorial Peptide Libraries
Chad J. Miller and Benjamin E. Turk


Titel: Kinase Screening and Profiling
Untertitel: Methods and Protocols
EAN: 9781493949236
ISBN: 1493949233
Format: Kartonierter Einband
Herausgeber: Springer New York
Anzahl Seiten: 236
Gewicht: 453g
Größe: H254mm x B178mm x T12mm
Jahr: 2016
Auflage: Softcover reprint of the original 1st ed. 2016

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