This book explains the biological and chemical principles of recombinant DNA technology. It emphasizes techniques used to isolate and clone specific genes from bacteria, plants, and animals, and methods of scaling-up the formation of the gene product for commercial applications.

Zusammenfassung
This important reference/text provides technologists with the basic informationnecessary to interact scientifically with molecular biologists and get involved in scalinguplaboratory procedures and designing and constructing commercial plants.Requiring no previous training or experience in biology, Genetic EngineeringFundamentals explains the biological and chemical principles of recombinant DNAtechnology ... emphasizes techniques used to isolate and clone specific genes frombacteria, plants, and animals, and methods of scaling-up the formation of the geneproduct for commercial applications ... analyzes problems encountered in scaling-upthe microprocessing of biochemical procedures . .. includes an extensive glossary andnumerous illustrations ... identifies other resource materials in the field ... and more.Presenting the fundamentals of biochemistry and molecular biology to workers andstudents in other fields, this state-of-the-art reference/text is essentiai reading fortechnologists in chemistry and engineering; biomedical, chemical, electrical andelectronics, industrial, mechanical, manufacturing, design, plant, control, civil, genetic,and environmental engineers; chemists, botanists, and zoologists; and advancedundergraduate and graduate courses in engineering, biotechnology, and industrialmicrobiology.

Inhalt

Basic Components 2. The Cell 3. DNA, RNA, and Genes 4. Protein Synthesis 5. Enzymes 6. Plasmids, Viruses, and Microbial HostsVectors 7. Recombinant Techniques 8. Nucleotide Sequencing and Hybridizing 9. Plant Genetics 10. Genetic Engineering Activities 11. Technology and Design 12. Techniques with Mammalian cells 13. Precautions and Regulations 14. Update