Inflammation and Cancer

One out of seven newly diagnosed malignancies worldwide result from infection and chronic inflammation in conjunction with cancer. This book is a guide for fighting the 2,200 inflammation-related oncogenesis fatalities that occur every day.

According to the most recent clinical oncology data, one out of seven newly diagnosed malignancies worldwide result from infection and chronic inflammation in conjunction with cancer. In Inflammation and Cancer: Methods and Protocols, expert researchers deliver a systematic guide to techniques addressing various aspects of experimental cancer biology, selectively focused on inflammation-mediated tumorigenesis, while promoting improvisations on a per-case basis. Volume 2, Molecular Analysis and Pathways is devoted to an extensive description of experimental strategies aimed at investigating the molecular cross-talks between components of cell signaling chains and their ramifications in diagnostic development and drug target discovery. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their subjects, lists of the necessary materials and reagents, step-by-step laboratory protocols, and a notes section, which examines tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Inflammation and Cancer: Methods and Protocols promises to serve as a vital guide and resource for investigators and clinicians working toward the goal of combatting the estimated 2, 200 inflammation-related oncogenesis fatalities occurring every day.

Contains key readily reproducible experimental approaches certain to be useful as technologies continue to develop Details basic requirements for the most recent techniques still awaiting experimental substantiation Fosters rigorous scientific interactions among basic and clinical researchers aimed at further molecular demarcation of the elaborate pathways leading from inflammation to tumor formation Describes in-depth pathway analysis, instrumental to the construction and navigation through molecular charts for cellular processes from gametogenesis through cell senescence Includes supplementary material: sn.pub/extras

Inhalt
Crossroads of Inflammation and Cancer: Molecular Aspects.- Immunohistochemical Analysis of 8-Nitroguanine, A Nitrative DNA Lesion, in Relation to Inflammation-Associated Carcinogenesis.- Molecular Analysis of Genetic Instability Caused by Chronic Inflammation.- Obesity Provides a Permissive Milieu in Inflammation-Associated Carcinogenesis: Analysis of Insulin and IGF Pathways.- Detecting and Modulating the NF-kB Activity in Human Immune Cells: Generation of Human Cell Lines with Altered Levels of NF-?B.- Inflammation-Related Aberrant Patterns of DNA Methylation: Detection and Role in Epigenetic Deregulation of Cancer Cell Transcriptome.- cDNA Microarray Analysis of Inflammatory Breast Cancer Signatures.- Analysis of the TLR/NF-?B Pathway in Antigen-Presenting Cells in Malignancies Promoted by Inflammation.- Induced Nitric Oxide Synthase as a Major Player in the Oncogenic Transformation of Inflamed Tissue.- Molecular and Cellular Approaches to Diagnostics and Drug Target Discovery In Inflammation-Related Oncogenesis.- Development of a Cell-Based Assay to Quantify the Inflammatory Potential of Test Substances and Screen Compound Libraries for Anti-cancer Drug Candidates in a High-Throughput Format.- The NF-?B Transcription Factor Pathway as a Therapeutic Target in Cancer: Methods for Detection of NF-?B Activity.- Inhibiting Proinflammatory NF-?B Signaling Using Cell-Penetrating NEMO Binding Domain Peptides.- Quantitative Measurement of Cytokine Expression in Synoviocytes Derived from Rheumatoid Arthritis Patients.- Molecular Characterization of Inflammation-Induced JNK/c-Jun Signaling Pathway in Connection with Tumorigenesis.- Molecular Characterization of STAT Signaling in Inflammation and Tumorigenesis.- Inflammation and Tumor Progression: A Lesson fromTNF-?-Dependent FAK Signaling in Cholangiocarcinoma.- Cyclooxygenase Inhibitors Induce Colon Cancer Cell Apoptosis Via PPAR? ? 14-3-3? Pathway.- PPAR?/? Agonist Increases the Expression of PGE2 Receptor Subtype EP4 in Human Lung Carcinoma Cells.- Investigating Macrophage and Malignant Cell Interactions In Vitro.- Cellular Magnetic Resonance Imaging Using Superparamagnetic Anionic Iron Oxide Nanoparticles: Applications to In Vivo Trafficking of Lymphocytes and Cell-Based Anticancer Therapy.- Application of Phage Display to Discovery of Tumor-Specific Homing Peptides: Developing Strategies for Therapy and Molecular Imaging of Cancer.- Molecular Basis of Histone Deacetylase Inhibitors as New Drugs for the Treatment of Inflammatory Diseases and Cancer.- Peptide YY Mediates Inhibition of Tumor Growth and Inflammation.