Immunological Tolerance

Immunological Tolerance: Methods and Protocols is a comprehensive guide to the techniques currently used for culturing and characterising the cell types responsible for imposing self-tolerance and the experimental models employed to study their function both in vitro and in vivo.

Provides up-to-date reviews of the literature on essential topics in immunological tolerance Documents methods for the generation and culture of critical cell types and approaches to their characterization Describes protocols for the study of tolerance both in vitro and in vivo Provides insights into novel techniques for tolerance induction

Inhalt
Cell Types Contributing to Immunological Tolerance.- Frontiers of Immunological Tolerance.- Balancing Tolerance and Immunity.- Differentiation of Dendritic Cell Subsets from Mouse Bone Marrow.- Genetic Modification of Dendritic Cells Through the Directed Differentiation of Embryonic Stem Cells.- Generation of Immunocompetent T Cells from Embryonic Stem Cells.- Isolation, Expansion, and Characterization of Human Natural and Adaptive Regulatory T Cells.- Derivation, Culture, and Characterization of Thymic Epithelial Cell Lines.- The Study of Immunological Tolerance In Vitro.- Thymus Organogenesis and Development of the Thymic Stroma.- Generation of a Tissue-Engineered Thymic Organoid.- Studying T-Cell Repertoire Selection Using Fetal Thymus Organ Culture.- Investigating Central Tolerance With Reaggregate Thymus Organ Cultures.- Estimating Thymic Function Through Quantification of T-Cell Receptor Excision Circles.- Gene Expression Profiling of Dendritic Cells by Microarray.- SAGE Analysis of Cell Types Involved in Tolerance Induction.- Analyzing the Physicodynamics of Immune Cells in a Three-Dimensional Collagen Matrix.- The Study of Immunological Tolerance In Vivo.- Etiology of Autoimmmune Disease.- Animal Models of Spontaneous Autoimmune Disease.- Antigen-Based Therapy and Immune-Regulation in Experimental Autoimmune Encephalomyelitis.- Induction and Regulation of Inflammatory Bowel Disease in Immunodeficient Mice by Distinct CD4+ T-Cell Subsets.- In Vivo Models for the Study of Transplantation Tolerance.- Ectopic Transplantation of Tissues Under the Kidney Capsule.- Intravital Two-Photon Imaging of T-Cell Priming and Tolerance in the Lymph Node.- Tracing Tolerance and Immunity In Vivo by CFSE-Labeling of Administered Cells.- Methods for Inducing and Breaking Immunological Tolerance.- Thymic Involution.- Inducing Mixed Chimerism and Transplantation Tolerance Through Allogeneic Bone Marrow Transplantation With Costimulation Blockade.- Induction of Dominant Tolerance Using Monoclonal Antibodies.- Induction of Tolerance by Adoptive Transfer of Treg Cells.- Modulation of the Immune Response Using Dendritic Cell-Derived Exosomes.- Breaking Self-Tolerance to Tumor-Associated Antigens by In Vivo Manipulation of Dendritic Cells.