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Heterologous Expression of Membrane Proteins

  • Fester Einband
  • 322 Seiten
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Beschreibung

This detailed volume encompasses chapters from leading experts in the area of membrane proteins who describe step-by-step protocols developed these last few years to improve the functional production and stabilization of recombinant integral membrane proteins (IMPs). Membrane proteins play a key role in numerous pathologies such as cancer, cystic fibrosis, epilepsy, hyperinsulinism, and Alzheimer's disease, yet studies on these and other disorders are hampered by a lack of information about the proteins involved. This book sets out to aid researchers in rectifying this situation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and up-to-date, Heterologous Expression of Membrane Proteins: Methods and Protocols, Second Edition serves as an ideal guide for scientists attempting to delve deeper into the myriad unique IMP structures.



Includes cutting-edge methods and protocols for the study of recombinant integral membrane

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts



Klappentext

This detailed volume encompasses chapters from leading
experts in the area of membrane proteins who describe step-by-step protocols developed these last few years to improve the functional
production and stabilization of recombinant integral membrane proteins (IMPs). Membrane
proteins play a key role in numerous pathologies such as cancer, cystic
fibrosis, epilepsy, hyperinsulinism, and Alzheimer's disease, yet studies on
these and other disorders are hampered by a lack of information about the
proteins involved. This book sets out to aid researchers in rectifying this
situation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions
to their respective topics, lists of the necessary materials and reagents,
readily reproducible laboratory protocols, and tips on troubleshooting and
avoiding known pitfalls.


Authoritative and up-to-date, Heterologous
Expression of Membrane Proteins: Methods and Protocols, Second Edition
serves as an ideal guide for scientists attempting to delve deeper into the myriad
unique IMP structures.



Zusammenfassung
This detailed volume encompasses chapters from leadingexperts in the area of membrane proteins who describe step-by-step protocols developed these last few years to improve the functionalproduction and stabilization of recombinant integral membrane proteins (IMPs).

Inhalt
1. Cell-Free Production of Membrane Proteins in Escherichia coli Lysates for Functional and Structural Studies Ralf-Bernhardt Rues, Erik Henrich, Coilin Boland, Martin Caffrey, and Frank Bernhard 2. Membrane Protein Production in the Yeast, S. cerevisiae Stephanie P. Cartwright, Lina Mikaliunaite, and Roslyn M. Bill 3. Membrane Proteins Production in Escherichia coli: Protocols and Rules Federica Angius, Oana Ilioaia, Marc Uzan, and Bruno Miroux 4. Codon Optimizing for Increased Membrane Protein Production: A Minimalist Approach Kiavash Mirzadeh, Stephen Toddo, Morten H.H. Nørholm, and Daniel O. Daley 5. Generation of Tetracycline-Inducible Mammalian Cell Lines by Flow Cytometry for Improved Overproduction of Membrane Proteins Juni Andréll, Patricia C. Edwards, Fan Zhang, Maria Daly, and Christopher G. Tate 6. Membrane Protein Production in Lactococcus lactis for Functional Studies Daphne Seigneurin-Berny, Martin S. King, Emiline Sautron, Lucas Moyet, Patrice Catty, François André, Norbert Rolland, Edmund R.S. Kunji, and Annie Frelet-Barrand 7. Expression of Viral Envelope Glycoproteins in Drosophila melanogaster S2 Cells Renato Mancini Astray, Sandra Fernanda Suárez-Patiño, and Soraia Attie Calil Jorge 8. Leishmania tarentolæ as a Promising Tool for Expressing Polytopic and Multi-Transmembrane Spans Eukaryotic Membrane Proteins: The Case of the ABC Pump ABCG6 Lucia Gonzalez-Lobato, Vincent Chaptal, Jennifer Molle, and Pierre Falson 9. Overexpression, Membrane Preparation, and Purification of a Typical Multidrug ABC Transporter BmrA Benjamin Wiseman and Jean-Michel Jault 10. Expression of Eukaryotic Membrane Proteins in Pichia pastoris Lucie Hartmann, Valérie Kugler, and Renaud Wagner 11. Integral Membrane Protein Expression in Saccharomyces cerevisiae Rebba C. Boswell-Casteel, Jennifer M. Johnson, Robert M. Stroud, and Franklin A. Hays 12. High-Throughput Baculovirus Expression System for Membrane Protein Production Ravi C. Kalathur, Marinela Panganiban, and Renato Bruni 13. Small-Scale Screening to Large-Scale Over-Expression of Human Membrane Proteins for Structural Studies Sarika Chaudhary, Sukanya Saha, Sobrahani Thamminana, and Robert M. Stroud 14. Purification of Human and Mammalian Membrane Proteins Expressed in Xenopus laevis Frog Oocytes for Structural Studies Rajendra Boggavarapu, Stephan Hirschi, Daniel Harder, Marcel Meury, Zöhre Ucurum, Marc J. Bergeron, and Dimitrios Fotiadis 15. 16. Detergent-Free Membrane Protein Purification Alice J. Rothnie 17. Conformational Dynamics and Interactions of Membrane Proteins by Hydrogen/Deuterium Mass Spectrometry Eric Forest and Petr Man 18. Lessons from an a-Helical Membrane Enzyme: Expression, Purification, and Detergent Optimization for Biophysical and Structural Characterization Jennifer L. Johnson, Sibel Kalyoncu, and Raquel L. Lieberman 19. Method to Screen Multidrug Transport Inhibitors Using Yeast Over-Expressing a Human MDR Transporter Laura Fiorini and Isabelle Mus-Veteau

Produktinformationen

Titel: Heterologous Expression of Membrane Proteins
Untertitel: Methods and Protocols
Editor:
EAN: 9781493936359
ISBN: 978-1-4939-3635-9
Format: Fester Einband
Herausgeber: Springer, Berlin
Genre: Biologie
Anzahl Seiten: 322
Gewicht: g
Größe: H24mm x B262mm x T185mm
Jahr: 2016
Auflage: 2. Aufl.

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