Handbook of ELISPOT

This third edition volume expands on the previous editions with new protocols and up-to-date techniques to help researchers excel in this field. The chapters in this book cover topics such as ELISpot's sibling (FluoroSpot); ELISpot for influenza, Dengue virus, and tuberculosis; and vaccine research, essentials control, and assay evaluation in ELISpot. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Handbook of ELISPOT: Methods and Protocols, Third Edition is a valuable resource for both novice researchers and experienced ELISpot users.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt
Challenges in Developing Protein Secretion Assays at a Single-Cell Level.- To Count of Not to Count: ELISPOT Evaluation Revisited.- Essential Controls for ELISpot Assay.- Automatic Search of Spots and Color Classification in ELISPOT Assay.- Four Color ImmunoSpot ® Assays for Identification of Effector T Cell Lineages.- Detection of Cross-Reactive B Cells Using the FluoroSpot Assay.- Multiplex ImmunoSpot® Assays for the Study of Functional B Cell Subpopulations.- Detecting All Immunoglobulin Classes and Subclasses in Multiplex 7 Color ImmunoSpot® Assays.- Multiplexing T- and B-Cell FLUOROSPOT Assays: Experimental Validation of the Multi-Color ImmunoSpot® Software Based on Center of Mass Distance Algorithm.- Multi-Color FLUOROSPOT Counting Using ImmunoSpot® Fluoro-X™ Suite.- B-Cell ELISpot Assay to Quantify Antigen-Specific Antibody-Secreting Cells in Human Peripheral Blood Mononuclear Cells.- Identification of Novel Mycobacterial Targets for Murine CD4+T Cells by IFN ELISPOT.- ELISPOT-Based Multi-Color FluoroSpot to Study Type-Specific and Cross-Reactive Responses in Memory B Cells after Dengue and Zika Virus Infections.- Cultured ELISpot Assay to Investigate Dengue Virus Specific T Cell Responses.- Ex Vivo ELISpot Assay to Investigate Dengue Virus Specific T Cell Responses.- Ex Vivo ELISpot Assay to Investigate iNKT Cell Responses in Acute Dengue Infection.- Dendritic Cell-Based ELISpot Assay for Assessing T Cell-IFN- Responses in Human Peripheral Blood Mononuclear Cells to Dengue Envelope Proteins.- Utilization of Feline ELISpot to Evaluate the Immunogenicity of a T Cell-Based FIV MAP Vaccines.- Detection and Quantification of Influenza A/H1N1 Virus-Specific Memory B Cells in Human PBMCs using ELISpot Assay.