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Impedance measurement techniques for the analysis of cell behavior

  • Kartonierter Einband
  • 120 Seiten
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A new micro-fluidic biosensor array for fast online adherent cell monolayer analysis during cell proliferation and stimulation was... Weiterlesen
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Beschreibung

A new micro-fluidic biosensor array for fast online adherent cell monolayer analysis during cell proliferation and stimulation was developed. Quartz crystal resonators and impedimetric sensor responses were analysed by means of impedance spectroscopy. Time-lapse light microscopy was employed for visual characterization of the cells. Four independent units embedded within the same device allow parallelized cell cultivation. An external flow injection system provides automated and parallelized media feed as well as overpressure regime in the system. Thin-film deposition techniques were applied for sensors fabrication. New dedicated sensor interface electronics were developed to allow fast and parallelized spectra acquisition on 32 sensors. Madin-Darby Canine Kidney cells were cultivated in the biosensor array. The cell behavior during cell proliferation and stimulation was analyzed online. It is believed that in the future, the new biosensor array can be successfully employed as a tool supporting standard techniques employed in molecular cell biology for the study of that complex system of communication that governs basic cellular activities and coordinates cell actions.

Autorentext

2007-2010: Wissenschaftlicher Mitarbeiter am Institut für Experimentelle Innere Medizin, Medizinische Fakultät der Otto-von-Guericke-Universität Magdeburg. Bearbeitung des DFG-Projectes "Disposable, micro-fluidic biosensor array for online analysis of adherent cells activity combining Quartz Crystal Resonators and Impedance measurement techniques".

Produktinformationen

Titel: Impedance measurement techniques for the analysis of cell behavior
Untertitel: Disposable, micro-fluidic biosensor array for online analysis of adherent cells activity
Autor:
EAN: 9783838129587
ISBN: 978-3-8381-2958-7
Format: Kartonierter Einband
Genre: Wärme- und Energietechnik
Anzahl Seiten: 120
Gewicht: 174g
Größe: H220mm x B220mm
Jahr: 2015
Auflage: Aufl.