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Genotyping

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This volume explores a broad range of different genotyping techniques. Genotyping: Methods and Protocols consists of chapters that... Weiterlesen
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Beschreibung

This volume explores a broad range of different genotyping techniques. Genotyping: Methods and Protocols consists of chapters that cover numerous topics such as: an overview of multiplexed microsatellite analysis; High Resolution Melt analysis and TaqMan-based assays; in situ analysis of variants in single RNA molecules; the MassARRAY system and Molecular Inversion Probes; Pulsed Field Gel Electrophoresis, Paralogue Ratio Test, and Multiplex Ligation-Dependent Probe Amplification; long-range PCR combined with PacBio sequencing; Targeted Locus Amplification; Multilocus Sequence Typing and rapid SNP detection with pyrosequencing; and genotyping-by-sequencing for plant analysis. Finally, the volume concludes with a summary of pertinent points to describe genetic variation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and practical, Genotyping: Methods and Protocols is a valuable resource for anyone interested in learning more about the diverse field of genotyping.



Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: sn.pub/extras



Klappentext

This volume explores a broad range of different genotyping techniques. Genotyping: Methods and Protocols consists of chapters that cover numerous topics such as: an overview of multiplexed microsatellite analysis; High Resolution Melt analysis and TaqMan-based assays; in situ analysis of variants in single RNA molecules; the MassARRAY system and Molecular Inversion Probes; Pulsed Field Gel Electrophoresis, Paralogue Ratio Test, and Multiplex Ligation-Dependent Probe Amplification; long-range PCR combined with PacBio sequencing; Targeted Locus Amplification; Multilocus Sequence Typing and rapid SNP detection with pyrosequencing; and genotyping-by-sequencing for plant analysis. Finally, the volume concludes with a summary of pertinent points to describe genetic variation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and practical, Genotyping: Methods and Protocols is a valuable resource for anyone interested in learning more about the diverse field of genotyping.



Inhalt

1. Genetic Fingerprinting Using Microsatellite Markers in a Multiplex PCR Reaction: A Compilation of Methodological Approaches from Primer Design to Detection Systems
Jacqueline Krüger and Dorit Schleinitz

2. Genotyping DNA Variants with High Resolution Melting Analysis
Rolf H.A.M. Vossen

3. High-Throughput Genotyping with TaqMan Allelic Discrimination and Allele-Specific Genotyping Assays
Angelika Heissl, Barbara Arbeithuber, and Irene Tiemann-Boege

4. In Situ Single Molecule RNA Genotyping Using Padlock Probes and Rolling Circle Amplification
Tomasz Krzywkowski, Thomas Hauling, and Mats Nilsson

5. The MassARRAY® System for Targeted SNP Genotyping
Justine A. Ellis and Benjamin Ong

6. Targeted Capture and High-Throughput Sequencing Using Molecular Inversion Probes (MIPs)
Stuart Cantsilieris, Holly A. Stessman, Jay Shendure, and Evan E. Eichler

7. Analyzing Copy Number Variation Using Pulsed-Field Gel Electrophoresis: Providing a Genetic Diagnosis for FSHD
Richard J.L.F. Lemmers

8. Analysis of Copy Number Variation Using the Paralogue Ratio Test (PRT)
Edward J. Hollox

9. Genotyping Complex Copy Number Variation with Multiplex Ligation-Dependent Probe Amplification (MLPA)
Suzan de Boer and Stefan J. White

10. Analysis of Multiallelic CNVs by Emulsion Haplotype Fusion PCR
Jess Tyson and John A.L. Armour

11. Quantitative DNA Analysis Using Droplet Digital PCR
Rolf H.A.M. Vossen and Stefan J. White

12. Full-Length Mitochondrial-DNA Sequencing on the PacBio RSII and Full-Length MT-DNA Sequencing on the PacBio RSII
Rolf H.A.M. Vossen and Henk P.J. Buermans

13. Targeted Locus Amplification and Next Generation Sequencing
Quint P. Hottentot, Max van Min, Erik Splinter, Stefan J. White

14. Efficient, Cost-Effective, High-Throughput, Multilocus Sequencing Typing (MLST) Method, NGMLST, and the Analytical Software Program MLSTEZ
Yuan Chen and John R. Perfect

15. Rapid SNP Detection and Genotyping of Bacterial Pathogens by Pyrosequencing
Kingsley K. Amoako, Matthew C. Thomas, Timothy W. Janzen, and Noriko Goji

16. Methods for Genotyping-by-Sequencing
Beth A. Rowan, Danelle K. Seymour, Eunyoung Chae, Derek S. Lundberg, and Detlef Weigel

17. Describing Sequence Variants Using HGVS Nomenclature
Johan T. den Dunnen

Produktinformationen

Titel: Genotyping
Untertitel: Methods and Protocols
Editor:
EAN: 9781493964406
ISBN: 978-1-4939-6440-6
Format: Fester Einband
Herausgeber: Springer, Berlin
Genre: Medizin
Anzahl Seiten: 254
Gewicht: 688g
Größe: H21mm x B262mm x T179mm
Jahr: 2016
Untertitel: Englisch
Auflage: 1st ed. 2017

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