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Genomics Protocols

  • Kartonierter Einband
  • 444 Seiten
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Since the first edition, published in 2001, genomics research has taken great strides. In this updated second edition, a team of e... Weiterlesen
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Since the first edition, published in 2001, genomics research has taken great strides. In this updated second edition, a team of expert researchers share the most current information in a field that has recently switched emphasis from gene identification to functional genomics and the characterization of genes and gene products. This volume approaches its subject with a broad perspective to supply its reader with a vital overview of genomics and its derivative fields.

Unparalleled diversity of technologies covered in a single volume

Supplies the reader with a vital overview of genomics and its derivative fields, with a focus on pivotal issues such as data analysis

Excellent Notes section, which offers tips on troubleshooting and avoiding known pitfalls

Includes supplementary material: sn.pub/extras

Contents Preface Contributors 1. Whole Genome Amplification with Phi29 DNA Polymerase to enable Genetic or Genomic Analysis of Samples of Low DNA Yield Kaisa Silander 2. Scanning for DNA variants by denaturant capillary electrophoresis Per O. Ekstrøm.. 3. Identification of SNPs, or Mutations in Sequence Chromatograms Nicole Draper. 4. Beadarray based Genotyping Helen Butler and Jiannis Ragoussis 5. Microsatellite-Based Candidate Gene Linkage Analysis Studies Cathryn Mellersh... 6. Full Complexity Genomic Hybridization on 60mer Oligonucleotide Microarrays for Array Comparative Genomic Hybridization (aCGH) Alexei Protopopov, Bin Feng, and Lynda Chin 7. Detection of Copy Number Changes at Multiple Loci in DNA Prepared from Formalin Fixed, Paraffin-Embedded Tissue by Multiplex Ligation- Dependent Probe Amplification Minoru Takata 8. Application of Microarrays for DNA Methylation Profiling Axel Schumacher, Andreas Weinhäusl, and Arturas Petronis 9. Genome-Wide Identification of Protein Binding Locations using Chromatin Immunoprecipitation coupled with Microarray Byung-Kwan Cho, Eric M. Knight, and Bernhard Ø. Palsson.. 10. Transcriptional Profiling of Small Samples in the CentralNervous System Stephen D. Ginsberg 11. Quantitative Expression Profiling of RNA from Formalin-Fixed, Paraffin- Embedded Tissues using randomly assembled Bead Arrays Marina Bibikova, Joanne M. Yeakley, Jessica Wang-Rodriguez, and Jian-Bing Fan . 12. Expression Profiling of microRNAs in Cancer Cells: Technical Considerations Mouldy Sioud and Lina Cekaite. 13. Identification of Disease Biomarkers by Profiling of Serum Proteins using SELDI-TOF Mass Spectrome Arsalan S. Haqqani, John F. Kelly and Danica B. Stanimirovic 18. Using 2D-LC-MS/MS to identify Francisella tularensis peptides in extracts from an infected mouse macrophage cell line John Kelly and Wen Ding 19. Baculovirus Expression Vector Systems (BEVS): An Emerging Host for High- Throughput Eukaryotic Protein Expression Binesh Shrestha, Carol Smee, and Opher Gileadi . 20. Co-immunoprecipitation and proteomic analyses S. Fabio Falsone, Bernd Gesslbauer and Andreas J. Kungl... 21. Tandem affinity purification combined with mass spectrometry to identify components of protein complexes Peter Kaiser, David Meierhofer, Xiaorong Wang and Lan Huang. 22. Mammalian Two-Hybrid Assay For Detecting Protein-Protein Interactions in vivo Runtao He and Xuguang Li.... 23. Detection of Protein-Protein Interactions in Live Cells and Animals with Split Firefly Luciferase Protein Fragment


Titel: Genomics Protocols
EAN: 9781617378188
ISBN: 1617378186
Format: Kartonierter Einband
Herausgeber: Humana Press
Anzahl Seiten: 444
Gewicht: 668g
Größe: H235mm x B155mm x T23mm
Jahr: 2010
Untertitel: Englisch
Auflage: Softcover reprint of hardcover 2nd ed. 2008

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