Gene Therapy of Cancer

Gene therapy has acquired a huge amount of new pre-clinical and clinical data. This book fully updates the first edition with thorough coverage of established and novel protocols involving both experimental and clinical approaches to cancer gene therapy.

Despite various difficulties, the field of gene therapy, particularly with regard to cancer, has accumulated a tremendous amount of vital pre-clinical and clinical data. "Gene Therapy of Cancer: Methods and Protocols, Second Edition" fully updates the first edition with expert coverage of established and novel protocols involving both experimental and clinical approaches to cancer gene therapy. This state-of-the-art volume contains overviews of new concepts and strategies with chapters on regulatory and ethical issues, developments, problems and possible limitations of design and production of gene therapeutics as well as translational issues. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Cutting edge and authoritative, "Gene Therapy of Cancer: Methods and Protocols, Second Edition" is an ideal guide for all those who wish to explore the fast-paced and critical study of nonviral, viral, experimental and clinical cancer gene therapy.

Describes a broad array of different strategies and methods such as for immunogene therapy, antitumor vaccination, oncolytic virotherapy, and suicide gene therapy

Provides state-of-the art protocols and overview chapters for cancer gene therapy, along with step-by-step procedures and instructions for gene therapeutic strategies, including notes and tips for troubleshooting to avoid pitfalls

Explores nonviral and viral vectors and relevant gene transfer/delivery systems

Includes methods and backgrounds of experimental and clinical gene therapy approaches

Covers the important issue of vector design and vector production for clinical applications

Features the current regulatory and ethical aspects in translating experimental approaches into clinical application of gene therapy to patients

Includes supplementary material: sn.pub/extras

Inhalt
Section I: Experimental Approaches in Cancer Gene Therapy Subsection A: Introduction 1. The Development of Gene Therapy: From Monogenic Recessive Disorders to Complex Diseases such as Cancer Jean-Pierre Gillet, Benjamin Macadangdang, Robert L. Fathke, Michael M. Gottesman, and Chava Kimchi-Sarfaty Subsection B: Vectorology for Cancer Gene Therapy 2. Designing Adenoviral Vectors for Tumor-Specific Targeting Ramon Alemany 3. Analysis of HSV Oncolytic Virotherapy in Organotypic Cultures Giulia Fulci and Brent Passer 4. Use of Minicircle Plasmids for Gene Therapy Peter Mayrhofer, Martin Schleef, and Wolfgang Jechlinger 5. Transposable Elements as Plasmid-Based Vectors for Long-Term Gene Transfer into Tumors John R. Ohlfest, Zoltán Ivics, and Zsuzsanna Izsvák 6. Designing Plasmid Vectors Oleg Tolmachov 7. Development of Bacterial Vectors for Tumor-Targeted Gene Therapy Li-Jun Jia and Zi-Chun Hua Subsection C: Nonviral Transfer Technologies in Cancer Gene Therapy 8. Electroporative Gene Transfer Marco Schmeer 9. Gene Gun Delivery Systems for Cancer Vaccine Approaches Kandan Aravindaram and Ning Sun Yang 10. Ultrasound-Mediated Gene Transfection Loreto B. Feril, Jr. 11. Nonviral Jet-Injection Technology for Intratumoral in vivo Gene Transfer of Naked DNA Wolfgang Walther, Iduna Fichtner, Peter M. Schlag, and Ulrike S. Stein Subsection D: Experimental Studies in Cancer Gene Therapy 12. Methods for Constructing and Evaluating Anti-Tumor DNA Vaccines Brian M. Olson and Douglas G. McNeel 13. Immunity of Lentiviral Vector-Modified Dendritic Cells Shuhong Han andLung-Ji Chang 14. Saporin Suicide Gene Therapy Natasa Zarovni, Riccardo Vago, and Maria Serena Fabbrini 15. Utilizing In Vivo Biopanning for the Development of Radiation-Guided Drug Delivery Systems Jerry J. Jaboin, Zhaozhong Han, and Dennis E. Hallahan 16. Chemosensitization of Tumor Cells: Inactivation of Nuclear Factor kB Associated with Chemosensitivity in Melanoma Cells after the Combination Treatment of E2F-1 and Doxorubicin Hongying Hao, H. Sam Zhou, and Kelly M. McMasters 17. Induction of Tumor Cell Apoptosis by TRAIL Gene Therapy Thomas S. Griffith 18. Silencing Epidermal Growth Factor Receptor (EGFR) by RNA Interference (RNAi) in Glioma Chunsheng Kang, Peiyu Pu, and Hao Jiang 19. Delivery of Phosphorodiamidate Morpholino Antisense Oligomers in Cancer Cells Gayathri R. Devi 20. Use of RNA Aptamers for the Modulation of Cancer Cell Signaling Sunjoo Jeong, Hee Kyu Lee, and Mee Young Kim 21. G-Rich Oligonucleotides (GROs) for Cancer Treatment Paula J. Bates, Enid W. Choi, and Lalitha V. Nayak Section II: Clinical Approaches in Cancer Gene Therapy Subsection A: Requirements for Clinical Gene Therapy Trials 22. Regulatory Aspects for Translating Gene Therapy Research into the Clinic Carolyn M. Laurencot and Sheryl Ruppel 23. Ethics of Cancer Gene Transfer Clinical Research Jonathan Kimmelman 24. Virus Production for Clinical Gene Therapy Tiago Vicente, Cristina Peixoto, and Manuel J. T. Carrondo 25. Production of Plasmid DNA as Pharmaceutical Martin Schleef and Markus Blaesen Subsection B: Clinical Protocols in Cancer Gene Therapy 26. Gene Immunotherapy for Non-Small Cell Lung Cancer John