In this book, experts present a broad range of available and developing techniques for the analysis of gene expression at the DNA,...
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In this book, experts present a broad range of available and developing techniques for the analysis of gene expression at the DNA, RNA, and protein levels. A collection of clearly described and illustrated chapters includes tips and implementation advice.
Understanding gene expression and how it changes under normal and pathological conditions is essential to our understanding of the fundamentals of cell biology through to the targeted treatment of disease. In Gene Expression Profiling: Methods and Protocols, Second Edition, experts in their particular fields compile detailed protocols for a broad range of techniques, currently available and being further developed, for the analysis of gene expression at the DNA, RNA, and protein levels. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and easy-to-use, Gene Expression Profiling: Methods and Protocols, Second Edition presents a collection of clearly described and illustrated chapters, certain to be helpful to researchers in academia, in hospitals, and in industry who are interested in applying techniques, whether basic or advanced, for the analysis of gene expression. Inhalt 1. Real-Time PCR and Multiplex Approaches Olga L. Gurvich and Mikhail Skoblov 2. Reverse-Transcriptase Polymerase Chain Reaction to Detect Extracellular mRNAs Sweta Rani and Lorraine O'Driscoll 3. Microarray Analysis of mRNAs: Experimental Design and Data Analysis Fundamentals Jai Prakash Mehta 4. Software and Tools for Microarray Data Analysis Jai Prakash Mehta and Sweta Rani 5. Analysis of Gene Expression as Relevant to Cancer Cells and Circulating Tumor Cells Anne M. Friel, John Crown, and Lorraine O'Driscoll 6. Gene Expression Profiling in Formalin-Fixed, Paraffin-Embedded Tissues Using the Whole-Genome DASL Assay Craig S. April and Jian-Bing Fan 7. MicroRNA Expression Analysis: Techniques Suitable for Studies of Intercellular and Extracellular MicroRNAs Erica Hennessy and Lorraine O'Driscoll 8. Western Blotting Analysis as a Tool to Study Receptor Tyrosine Kinases Serena Germano and Lorraine O'Driscoll 9. 2D Gel Electrophoresis and Mass Spectrometry Identification and Analysis of Proteins Paula Meleady 10. Design, Construction, and Analysis of Cell Line Arrays and Tissue Microarrays for Gene Expression Analysis Kathy Gately, Keith Kerr, and Ken O'Byrne 11. Immunohistochemical and Immunofluorescence Procedures for Protein Analysis Kishore Reddy Katikireddy and Finbarr O'Sullivan 12. Advanced Microscopy: Laser Scanning Confocal Microscopy Orla Hanrahan, James Harris, and Chris Egan 13. Isolation of Exosomes for Subsequent mRNA, MicroRNA, and Protein Profiling Sweta Rani, Keith O'Brien, Fergal C. Kelleher, Claire Corcoran, Serena Germano, Marek Radomski, John Crown, and Lorraine O'Driscoll 14. Atomic Force Microscopy (AFM) and High-Content Analysis (HCA): Two Innovative Technologies for Dissecting the Relationship Between Epithelial-Mesenchymal Transition (EMT)-Related Morphological and Structural Alterations and Cell Mechanical Properties Stephen T. Buckley, Anthony M. Davies, and Carsten Ehrhardt