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Gap Junction Protocols

  • Fester Einband
  • 252 Seiten
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Presenting state-of-the-art protocols to study gap junctions, this detailed book first focuses on the use of methods and tools to ... Weiterlesen
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Presenting state-of-the-art protocols to study gap junctions, this detailed book first focuses on the use of methods and tools to investigate the different aspects of connexin expression and gap junction regulation. The second part of the volume describes several methods to probe gap junction functionality as such. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Gap Junction Protocols is intended for basic and applied researchers in the area of biomedical and life sciences, both in academic and industrial settings.

Includes cutting-edge methods and protocols involving gap junctions

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

1. Analysis of Liver Connexin Expression Using Reverse Transcription Quantitative Real-Time Polymerase Chain Reaction Michaël Maes, Joost Willebrords, Sara Crespo Yanguas, Bruno Cogliati, and Mathieu Vinken 2. DNA Methylation Analysis of Human Tissue-Specific Connexin Genes Xiaoming Liu and Canxia Xu 3. Detection of Connexins in Liver Cells Using Sodiumdodecylsulfate Polyacrylamide Gel Electrophoresis and Immunoblot Analysis Joost Willebrords, Michaël Maes, Sara Crespo Yanguas, Bruno Cogliati, and Mathieu Vinken 4. Immunohisto- and Cytochemistry Analysis of Connexins Bruno Cogliati, Michaël Maes, Isabel Veloso Alves Pereira, Joost Willebrords, Tereza Cristina da Silva, Sara Crespo Yanguas, and Mathieu Vinken 5. Small Interfering RNA-Mediated Connexin Gene Knockdown in Vascular Endothelial and Smooth Muscle Cells Miranda E. Good, Daniela Begandt, Leon J. DeLalio, Scott R. Johnstone, and Brant E. Isakson 6. Generation and Use of Trophoblast Stem Cells and Uterine Myocytes to Study the Role of Connexins for Pregnancy and Labor Mark Kibschull, Stephen J. Lye, and Oksana Shynlova 7. Identification of Connexin43 Phosphorylation and S-Nitrosylation in Cultured Primary Vascular Cells Alexander W. Lohman, Adam C. Straub, and Scott R. Johnstone 8. Preparation of Gap Junctions in Membrane Microdomains for Immunoprecipitation and Mass Spectrometry Interactome Analysis Stephanie Fowler, Mark Akins, and Steffany A.L. Bennett 9. Scrape Loading/Dye Transfer Assay Pavel Babica, Iva Sovadinová, and Brad L. Upham 10. Microinjection Technique for Assessment of Gap Junction Function Michael D. Fridman, Jun Liu, Yu Sun, and Robert M. Hamilton 11. Electroporation Loading and Dye Transfer: A Safe and Robust Method to Probe Gap Junctional Coupling Elke Decrock, Marijke De Bock, Diego De Baere, Delphine Hoorelbeke, Nan Wang, and Luc Leybaert 12. Using Fluorescence Recovery After Photobleaching to Study Gap Junctional Communication In Vitro Maria Kuzma-Kuzniarska, Clarence Yapp, and Philippa A. Hulley 13. Tracking Dynamic Gap Junctional Coupling in Live Cells by Local Photo-Activation and Fluorescence Imaging Song Yang and Wen-Hong Li 14. A Functional Assay to Assess Connexin43-Mediated Cell-to-Cell Communication of Second Messengers in Cultured Bone Cells Joseph P. Stains and Roberto Civitelli 15. Calcium Wave Propagation Triggered by Local Mechanical Stimulation as a Method for Studying Gap Junctions and Hemichannels Jegan Iyyathurai, Bernard Himpens, Geert Bultynck, and Catheleyne D'hondt 16. Establishment of the Dual Whole Cell Recording Patch Clamp Configuration for the Measurement of Gap Junction Conductance Richard D. Veenstra


Titel: Gap Junction Protocols
EAN: 9781493936625
ISBN: 149393662X
Format: Fester Einband
Herausgeber: Springer New York
Anzahl Seiten: 252
Gewicht: 678g
Größe: H260mm x B183mm x T19mm
Jahr: 2016
Untertitel: Englisch
Auflage: 1st ed. 2016

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