Functional Genomics

Includes fully updated, cutting-edge techniques for the study of functional genomics
Provides step-by-step detail essential for reproducible results
Contains key implementation advice from the experts

Inhalt
Predicting RNA Structure with Vfold.- RNA Function Prediction.- Computational Prediction of Novel miRNAs from Genome-Wide Data.- Protein Structure Modeling with MODELLER.- Protein Function Prediction.- Capturing Three-Dimensional Genome Organization in Individual Cells by Single-Cell Hi-C.- Genome-Wide Cell Type-Specific Mapping of In Vivo Chromatin Protein Binding Using an FLP-Inducible DamID System in Drosophila.- DNA Methylation Profiling Using Long-Read Single-Molecule Real-Time Bisulfite Sequencing (SMRT-BS).- Copy Number Variation Analysis by Droplet Digital PCR.- MicroScale Thermophoresis: A Rapid and Precise Method to Quantify Protein-Nucleic Acid Interactions in Solution.- Establishment of the CRISPR/Cas9 System for Targeted Gene Disruption and Gene Tagging.- Holistic and Affordable Analyses of MicroRNA Expression Profiles Using Tagged cDNA Libraries and a Multiplex Sequencing Strategy.- MicroRNA Expression Analysis Using Small RNA Sequencing Discovery and RT-qPCR-Based Validation.- Using Firefly ® Particle Technology for Multiplex MicroRNA Profiling Without RNA Purification.- Multiplex Real-Time PCR Using Encoded Microparticles for MicroRNA Profiling.- Optimized Whole Transcriptome Profiling of Motor Axons.- 2D-DIGE in Proteomics.- STAGE-Diging in Proteomics.- Protein Arrays I: Antibody Arrays.- Protein Arrays II: Antigen Arrays.- Protein Arrays III: Reverse-Phase Protein Arrays.- Isolation of Exosomes for the Purpose of Protein Cargo Analysis with the Use of Mass Spectrometry.- Virus-Induced Gene Silencing (VIGS) and Foreign Gene Expression in Pisum sativum L. Using the 'One-Step' Bean pod mottle virus (BPMV) Viral Vector.- Re-Expressing Epigenetically Silenced Genes by Inducing DNA Demethylation through Targeting of Ten-Eleven Translocation 2 to Any Given Genomic Locus.- Knockdown of Rice MicroRNA166 by Short Tandem Target Mimic (STTM).- RNAi-Mediated Knockdown of Protein Expression.- Engineered Zinc Finger DNA-Binding Domains: Synthesis, Assessment of DNA-Binding Affinity, and Direct Protein Delivery to Mammalian Cells.- Production, Purification, and Titration of First-Generation Adenovirus Vectors. <p