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Fluorescence in situ Hybridization (FISH)

  • Kartonierter Einband
  • 468 Seiten
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In Fluorescence in situ Hybridization (FISH), experts in the field portray the vibrant complexity and diversity of the current FIS... Weiterlesen
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Beschreibung

In Fluorescence in situ Hybridization (FISH), experts in the field portray the vibrant complexity and diversity of the current FISH protocol landscape. The book provides cutting-edge examples of various applications for research and study purposes.


Fluorescence in situ Hybridization (FISH) belongs to that special category of well-established molecular biology techniques that, since their inception a few decades ago, have succeeded in keeping a prominent position within the constantly expanding list of laboratory pro- dures for biomedical research and clinical diagnostics. The design simplicity and cost-effectiveness of the early FISH protocols, combined with the signifcant acceleration of discoveries in related technical areas such as fuor- cence microscopy, digital imaging, and nucleic acid technology have prompted the div- sifcation of the original technique into an outstanding number of imaginative and useful applications, and thus have not only held back its outmoding but have also promoted its expansion into different areas of basic and applied research in the post-genomic era. The 34 chapters included in this book aim at portraying the vibrant complexity and diversity of the current FISH protocol landscape, providing cutting-edge examples of va- ous applications for genetic and developmental research, cancer research, reproductive medicine, diagnostic and prognostic purposes, microbial ecology, and evolutionary st- ies. The book is divided in four parts: (I) Core Techniques, (II) Technical Advancements and Novel Adaptations, (III) Translational FISH: Applications for Human Genetics and Medicine, and (IV) Protocols for Model Organisms.

Contains a plethora of modern adaptations to the FISH methodology

Of great use to both clinical and research laboratories

Will inform how to achieve and recognize quality in the different FISH methodologies



Klappentext
The design simplicity and cost-effectiveness of the early Fluorescence in situ Hybridization (FISH) protocols, combined with the significant acceleration of discoveries in related technical areas like fluorescence microscopy, digital imaging, and nucleic acid technology have prompted the diversification of the original technique into an outstanding number of imaginative and useful applications, thus promoting its expansion into different areas of basic and applied research in the post-genomic era. In Fluorescence in situ Hybridization (FISH): Protocols and Applications, experts in the field portray the vibrant complexity and diversity of the current FISH protocol landscape, providing cutting-edge examples of various applications for genetic and developmental research, cancer research, reproductive medicine, diagnostic and prognostic purposes, microbial ecology and evolutionary studies. The book is divided into four convenient sections covering the core techniques, technical advancements and novel adaptations, applications for human genetics and medicine, as well as protocols for model organisms. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective chapters, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes highlighting tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Fluorescence in situ Hybridization (FISH): Protocols and Applications aims to aid scientists from across the world in applying this tried-and-true scientific technique to their own lab's current research.

Inhalt
Part I: Core Techniques 1. Fluorescence in situ Hybridization (FISH), Basic Principles, and Methodology Elisa Garimberti and Sabrina Tosi 2. Fluorescence in situ Hybridization on DNA Halo Preparations and Extended Chromatin Fibers Lauren S. Elcock and Joanna M. Bridger 3. Detection of Nascent RNA Transcripts by Fluorescence in situ Hybridization Jill M. Brown and Veronica J. Buckle 4. Fluorescence in situ Hybridization Analysis of Formalin Fixed Paraffin Embedded Tissues, Including Tissue Microarrays Brenda M. Summersgill and Janet M. Shipley 5. Fluorescence in situ Hybridization (FISH) on Tissue Cryosections Irina Solovei 6. Multiplex Fluorescence in situ Hybridization (M-FISH) Rhona Anderson 7. Optical Mapping of Protein-DNA Complexes on Chromatin Fibers Beth Sullivan 8. 3D-FISH on Cultured Cells Combined with Immunostaining Irina Solovei and Marion Cremer Part II: Technical Advancements and Novel Adaptations 9. The Comet-FISH Assay for the Analysis of DNA Damage and Repair Graciela Spivak 10. Direct in situ Hybridization with Oligonucleotide Functionalized Quantum Dot Probes Laurent A. Bentolila 11. LNA-FISH for Detection of microRNAs in Frozen Sections Asli N. Silahtaroglu 12. Chromosome Orientation Fluorescence in situ Hybridization (CO-FISH) or Strand-Specific FISH Susan M. Bailey, Eli S. Williams, Michael N. Cornforth, and Edwin H. Goodwin 13. Combinatorial Oligo FISH: Directed Labeling of Specific Genome Domains in Differentially Fixed Cell Material and Live Cells Eberhard Schmitt, Jutta Schwarz-Finsterle, Stefan Stei, Carmen Boxler, Patrick Müller, Andriy Mokhir, Roland Krämer, Christoph Cremer, and Michael Hausmann 14. Simultaneous Visualization of FISH Signals and Bromo-deoxyuridine Incorporation by Formamide-Free DNA Denaturation Daniela Moralli and Zoia L. Monaco 15. CryoFISH: Fluorescence in situ Hybridization on Ultrathin Cryosections Sheila Q. Xie, Liron-Mark Lavitas, and Ana Pombo 16. Characterization ofChromosomal Rearrangements Using Multicolor-Banding (MCB/m-band) Thomas Liehr, Anja Weise, Sophie Hinreiner, Hasmik Mkrtchyan, Kristin Mrasek, and Nadezda Kosyakova 17. Visualizing Nucleic Acids in Living Cells by Fluorescence in vivo Hybridization Joop Wiegant, Anneke K. Brouwer, Hans J. Tanke, and Roeland W. Dirks Part III: Translational FISH: Applications for Human Genetics and Medicine 18. Quality Control in FISH as Part of a Laboratory's Quality Management System Ros Hastings 19. FlashFISH: Same Day Prenatal Diagnosis of Common Chromosomal Aneuploidies Sherry Ho and Mahesh A. Choolani 20. FISH for Pre-Implantation Genetic Diagnosis Paul N. Scriven and Caroline Mackie Ogilvie 21. PNA-FISH on Human Sperm Franck Pellestor, Cécile Monzo, and Samir Hamamah 22. POD-FISH: A New Technique for Parental Origin Determination Based on Copy Number Variation Polymorphism Anja Weise, Madeleine Gross, Sophie Hinreiner, Vera Witthuhn, Hasmik Mkrtchyan, and Thomas Liehr 23. Sequence-Based High Resolution Chromosomal Comparative Genomic Hybridization (CGH) Agata Kowalska, Eva Bozsaky, and Peter F. Ambros 24. ImmunoFISH on Isolated Nuclei from Paraffin-Embedded Biopsy Material Soo-Yong Tan and Goran Mattsson 25. Fluorescence in situ Hybridization on 3D Cultures of Tumor Cells Karen J. Meaburn 26. Simultaneous Ultrasensitive Subpopulation Staining/Hybridization in situ (SUSHI) in HIV-1 Disease Monitoring Bruce Patterson Part IV: Protocols for Model Organisms 27. Detection of Prokaryotic Cells with Fluorescence in situ Hybridization Katrin Zwirglmaier 28. FISH as a Tool to Investigate Chromosome Behavior in Budding Yeast Harry Scherthan and Josef Loidl 29. FISH on Chromosomes Derived from the Snail Model Organism Biomphalaria glabrata Edwin C. Odoemelam, Nithya Raghavan, Wannaporn Ittiprasert, Andre Miller, Joanna M. Bridger, and Matty Knight 30. Fluorescence in situ Hybridization with Bacterial Artificial Chromosomes (BACs) to Mitotic

Produktinformationen

Titel: Fluorescence in situ Hybridization (FISH)
Untertitel: Protocols and Applications
Editor:
EAN: 9781493957248
ISBN: 1493957244
Format: Kartonierter Einband
Herausgeber: Humana Press
Anzahl Seiten: 468
Gewicht: 873g
Größe: H254mm x B178mm x T25mm
Jahr: 2016
Auflage: Softcover reprint of the original 1st ed. 2010

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