Fast Detection of DNA Damage

This volume presents a comprehensive collection of quick assays for the detection of nuclear and mitochondrial DNA damage and its effects in live and fixed cells and tissues, and in bacterial genomes. Although, such rapid techniques are in demand in the research trenches they are not covered well in the literature. This volume is the first such compendium of the time-saving techniques for detection of DNA damage and its direct physiological outcomes including apoptosis, necrosis and phagocytic clearance. The volume demonstrates all levels of detection, starting from the molecular level up to the level of the entire live organism. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Fast Detection of DNA Damage: Methods and Protocols aims to provide easily reproducible techniques requiring only few steps to perform.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras

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Express FRET Labeling and Analysis of Phagocytic Clearance.- Rapid Assessment of Genotoxicity by Flow Cytometric Detection of Cell Cycle Alterations.- Ultrasound Imaging of DNA-Damage Effects in Live Cultured Cells and in Brain Tissue.- Ultrasound Imaging of Apoptosis: Spectroscopic Detection of DNA-Damage Effects In Vivo .- Fluorochrome-Labeled Inhibitors of Caspases: Expedient In Vitro and In Vivo Markers of Apoptotic Cells for Rapid Cytometric Analysis.- The Fast-halo Assay for the Detection of DNA Aamage.- Rapid Detection of Bacterial Susceptibility or Resistance to Quinolones.- Rapid Detection of Apoptosis in Cultured Mammalian Cells.- Quick Detection of DNase II-type Breaks in Formalin-Fixed Tissue Sections.- Express -H2AX Immunocytochemical Detection of DNA damage.- Rapid detection of -H2AX by Flow Cytometry in Cultured Mammalian Cells Firsanov.- Rapid Detection of DNA Strand Breaks in Apoptotic Cells by Flow- and Image- Cytometry.- Fast Micromethod: Determination ofDNA Integrity in Cell Suspensions and in Solid Tissues.- Quantification of DNA Damage and Repair in Mitochondrial, Nuclear, and Bacterial Genomes by Real-Time PCR.- Zebra Tail Amplification: Accelerated Detection of Apoptotic Blunt Ended DNA Breaks by In Situ Ligation.- Twelve Gel Comet Assay Format for Quick Examination of DNA Damage and Repair.- Immunofluorescence Analysis of -H2AX Foci in Mammalian Fibroblasts at Different Phases of the Cell Cycle.- RAPD-PCR as a Rapid Approach for the Evaluation of Genotoxin-Induced Damage to Bacterial DNA.- Rapid Detection of -H2Av Foci in ex vivo MMS-treated Drosophila Imaginal Discs.