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Estrogen Receptors

  • Fester Einband
  • 576 Seiten
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This volume examines a wide array of vital technologies for advancing our understanding of the receptor-mediated actions of estrog... Weiterlesen
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Beschreibung

This volume examines a wide array of vital technologies for advancing our understanding of the receptor-mediated actions of estrogen. The protocols in this book range from standard methods and vital laboratory workhorses, such as receptor binding assays and western blot, to newer technologies such as RNAseq and proximity ligation assay. Chapters also discuss protocols from a broad range of tissue types to demonstrate the variety of estrogen receptor effects. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Estrogen Receptors: Methods and Protocols is a detailed and helpful resource for scientists who are intrigued by the many facets of estrogen.
This volume examines a wide array of vital technologies for advancing our understanding of the receptor-mediated actions of estrogen. The protocols in this book range from standard methods and vital laboratory workhorses, such as receptor binding assays and western blot, to newer technologies such as RNAseq and proximity ligation assay. Chapters also discuss protocols from a broad range of tissue types to demonstrate the variety of estrogen receptor effects. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Estrogen Receptors: Methods and Protocols is a detailed and helpful resource for scientists who are intrigued by the many facets of estrogen.

The chapter 'Bioinformatics Analysis of Estrogen-Responsive Genes' is open access under a CC BY 4.0 license.



Autorentext

Kathleen (Kathy) Eyster Professor of Physiology and Pharmacology Sanford School of Medicine of the University of South Dakota 605-677-5159



Inhalt

1. The Estrogen Receptors: An Overview from Different Perspectives Kathleen M. Eyster 2. Competitive Binding Assay for the G-Protein Coupled Receptor 30 (GPR30) or G-Protein Coupled Estrogen Receptor (GPER) Thomas Thekkumkara, Russell Snyder, and Vardan T. Karamyan 3. The Use of Real Time Reverse Transcription-PCR for Assessing Estrogen Receptor and Estrogen-Responsive Gene Expression Michelle L. Booze and Kathleen M. Eyster 4. Bioinformatics Analysis of Estrogen-Responsive Genes Adam E. Handel 5. Electrophoretic Mobility Shift Assay (EMSA) and Supershift Assay of Cytochrome P450 2B6 in Response to Estrogen Kwi Hye Koh and Hyunyoung Jeong 6. Chromatin Immunoprecipitation Assay to Identify Genomic Binding Sites of Regulatory Factors Meike Wagner, Johannes Jung, Michael Koslowski, Özlem Türeci, Vijay K. Tiwari, and Ugur Sahin 7. Chromatin Immunoprecipitation with Estrogen Receptor 1 and the Promoter of Greb1 in TM4 Sertoli Cells Jing Lin and Zhenmin Lei 8. Chromatin Immunoprecipitation-Sequencing (ChIP-seq) for Mapping of Estrogen Receptor-Chromatin Interactions in Breast Cancer Kelly A. Holmes, Gordon D. Brown, and Jason S. Carroll 9. RNA-Seq Experiment and Data Analysis Hanquan Liang and Erliang Zeng 10. DNA Microarray Analysis of Estrogen-Responsive GenesKathleen M. Eyster 11. Shotgun Proteomics Analysis of Estrogen Effects in the Uterus Using Two Dimensional Liquid Chromatography and Tandem Mass Spectrometry Eduardo A. Callegari 12. Assessment of Protein Expression by Proximity Ligation Assay in the Nonhuman Primate Endometrium, Placenta, and Fetal Adrenal in Response to Estrogen Thomas W. Bonagura, Jeffery S. Babischkin, Gerald J. Pepe and Eugene D. Albrecht 13. Co-Localization of Estrogen Receptors with the Fluorescent Tamoxifen Derivative, FLTX1, Analyzed by Confocal Microscopy Araceli Morales, Raquel Marín, Jorge Marrero-Alonso, Alicia Boto, and Mario Díaz 14. Live Cell Imaging of the Estrogen Receptor by Total Internal Reflection Fluorescence Microscopy Kassandra Kisler and Reymundo Dominguez 15. In Situ Hybridization of Estrogen Receptors a and ß and GPER in the Human Testis D. Fietz, M. Bergmann, and K. Hartmann 16. Purification of Histone Lysine Methyltransferase SMYD2 and Co-Crystallization with a Target Peptide from Estrogen Receptor a Yuanyuan Jiang, Joshua Holcomb, Nicholas Spellmon, and Zhe Yang 17. Gold Nanoparticle-Based Förster Resonance Energy Transfer (FRET) Analysis of Estrogen Receptor: DNA Interaction Xiaodi Su, Khin Moh Moh Aung, Steven Lukman, and Bin Liu 18. Analysis of Interaction of Estradiol with Estrogen Receptor by NMR Spectroscopy M.K. Thakur and V. Paramanik 19. Application of Circular Dichroism Spectroscopy to the Analysis of the Interaction between the Estrogen Receptor Alpha and Co-Activators: The Case of Calmodulin Emeric Miclet, Sandrine Bourgoin-Voillard, Cillian Byrne, and Yves Jacquot 20. Surface Plasmon Resonance Study of Cooperative Interactions of Estrogen Receptor a and Specificity Protein 1 with Composite DNA Elements Xiaodi Su and Hong Yan Song 21. Tips and Tricks for Successful Application of Statistical Methods to Biological Data Evelyn Schlenker 22. The Synonymous Ala87 Mutation of Estrogen Receptor Alpha Modifies Transcriptional Activation through Both ERE and AP1 Sites Tamara Fernández-Calero, Gilles Flouriot, and Mónica Marín <23. Selective Estrogen Receptor Modulators and the Tissue-Selective Estrogen Complex: Analysis of Cell Type-Specific Effects using In Vivo Imaging of a Reporter Mouse Model Sara Della Torre and Paolo Ciana 24. Use of Reporter Genes to Analyze Estrogen Response: The Transgenic Zebrafish Model Daniel A. Gorelick, Caroline Lucia Pinto, Ruixin Hao, and Maria Bondesson

25. Comparison of the Effects of the Selective Estrogen Receptor Modulators Ospemifene, Raloxifene, and Tamoxifen on Breast Tissue in Ex Vivo CultureNatalija Eigeliene, Risto Erkkola, and Pirkko Härkönen 26. Estrogen Receptor Agonists and Antagonists in the Yeast Estrogen Bioassay Si Wang and Toine F. H. Bovee 27. Silencing Estrogen Receptor-a with siRNA in the Intact Rodent Brain Ana C. Ribeiro, Anders Ågmo, Sergei Musatov, and Donald W. Pfaff 28. Silencing Estrogen Receptor-ß with siRNA in Cultured Cells Ren-Jun Hsu and Jui-Ming Liu 29. Experimental Procedures for Demonstration of MicroRNA Mediated Enhancement of Functional Neuroprotective Effects of Estrogen Receptor Agonists Mrinmay Chakrabarti and Swapan K. Ray 30. Expression Profiles of Estrogen-Regulated MicroRNAs in Breast Cancer Cells Anne Katchy and Cecilia Williams 31. Estradiol-Induced Transcriptional Regulation of Long Non-Coding RNA, HOTAIR Arunoday Bhan and Subhrangsu S. Mandal 32. Detection and Functional Analysis of Estrogen Receptor a Phosphorylated at Serine 216 in Mouse Neutrophils Sawako Shindo, Rick Moore, and Masahiko Negishi 33. Estrogen Receptor-a Knockout Mice Per Antonson, Patricia Humire, and Jan-Åke Gustafsson 34. Detection of Endogenous Selective Estrogen Receptor Modulators such as 27-Hydroxycholesterol Erik R. Nelson 35. Phytoestrogens Activate the Estrogen Receptor in HepG2 CellsLynne A. Kelly 36. Detection of the Phosphorylation of the Estrogen Receptor a as an Outcome of GPR30 Activation Sara Clark, Kevin Pollard , Jennifer Rainville, and Nandini Vasudevan 37. GPER Mediates Non-Genomic Effects of Estrogen Marco Pupo, Marcello Maggiolini, and Anna Maria Musti 38. GPER/GPR30 Knockout Mice: Effects of GPER on Metabolism Geetanjali Sharma and Eric R. Prossnitz 39. Analysis of G-Protein Coupled Receptor 30 (GPR30) on Endothelial Inflammation Subhadeep Chakrabarti and Sandra T. Davidge 40. Atherosclerosis and Vascular Biologic Responses to Estrogens: Histologic, Immunohistochemical, Biochemical, and Molecular Methods Thomas C. Register, Susan E. Appt, and Thomas B. Clarkson 41. Assessing Direct Vascular Actions of Estrogens Douglas S. Martin, and Kathleen M. Eyster 42. Molecular Design, Synthesis, and Evaluation of SNIPER(ER) that Induces Proteasomal Degradation of ERa Keiichiro Okuhira, Yosuke Demizu, Takayuki Hattori, Nobumichi Ohoka, Norihito {Itoh, 2010 #4}Shibata, Masaaki Kurihara, and Mikihiko Naito 43. Regulation of Activation Induced Deaminase (AID) by Estrogen Siim Pauklin

Produktinformationen

Titel: Estrogen Receptors
Untertitel: Methods and Protocols
Editor:
EAN: 9781493931262
ISBN: 978-1-4939-3126-2
Format: Fester Einband
Herausgeber: Springer, Berlin
Genre: Medizin
Anzahl Seiten: 576
Gewicht: g
Größe: H28mm x B261mm x T185mm
Jahr: 2015
Auflage: 1st ed. 2016

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