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ERK Signaling

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This volume provides a collection of techniques and approaches for the study of ERK signaling. It begins with a historical perspec... Weiterlesen
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Beschreibung

This volume provides a collection of techniques and approaches for the study of ERK signaling. It begins with a historical perspective of genetic and molecular discoveries, followed by chapters covering specific topics in a broad range of experimental systems, including in vitro assays of EGFR and ERK activities; proteomic and genome-wide analyses of ERK signaling targets; cell biological, genetic, quantitative and imaging approaches in cells and model organisms; and integrated mathematical modeling of the ERK pathway. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and thorough, ERK Signaling: Methods and Protocols will aid and stimulate further advances in the vibrant field of ERK signaling.<

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains keynotes and implementation advice from the experts



Inhalt
1. How Genetics has Helped Piece Together the MAPK Signaling PathwayDariel Ashton-Beaucage and Marc Therrien2. In Vitro Enzyme Kinetics Analysis of EGFRZhihong Wang and Christine Candelora3. High-Throughput Analysis of Mammalian Receptor Tyrosine Kinase Activation in Yeast Cells Nobuo Yoshimoto and Shun'ichi Kuroda4. Structural Studies of ERK2 Protein Complexes Johannes F. Weijman, Stefan J. Riedl, and Peter D. Mace5. Isolation and Characterization of Intrinsically Active (MEK-Independent) Mutants of Mpk1/Erk Tal Goshen-Lago, Dganit Melamed, Arie Admon, and David Engelberg6. Assaying Activation and Subcellular Localization of ERK in Cells and Tissues Carme Caelles, Carles Bayod, and Melisa Morcillo7. Detection and Functional Analysis of SUMO-Modified MEK Yuji Kubota and Mutsuhiro Takekawa8. Single-Step Affinity Purification of ERK Signaling Complexes Using the Streptavidin-Binding Peptide (SBP) Tag Liu Yang and Alexey Veraksa9. High-Throughput In Vitro Identification of Direct MAPK/Erk Substrates Rona Grossman and Ze'ev Paroush10. Global Identification of ERK Substrates by Phosphoproteomics Based on IMAC and 2D-DIGE Hidetaka Kosako and Kou Motani11. Analysis of Ras/ERK Compartmentalization by Subcellular Fractionation Lorena AgudoIbañez, Piero Crespo, and Berta Casar12. Cell-Based Assays to Study ERK Pathway/Caveolin1 Interactions Raffaele Strippoli, Asier Echarri, Miguel Angel del Pozo13. The Nuclear Translocation of ERK Denise A. Berti and Rony Seger14. Visualization of RAS/MAPK Signaling In Situ by the Proximity Ligation Assay (PLA) Zijian Tang and Chengkai Dai15. Measuring ERK Activity Dynamics in Single Living Cells Using FRET Biosensors Yannick Blum, Rafael D. Fritz, Hyunryul Ryu, and Olivier Pertz16. Quantifying Tensile Force and ERK Phosphorylation on Actin Stress Fibers Hiroaki Hirata, Mukund Gupta, Sri Ram Krishna Vedula, Chwee Teck Lim, Benoit Ladoux, and Masahiro Sokabe17. Co-Culture Activation of MAP Kinase in Drosophila S2 Cells Josefa Steinhauer18. Isolation of Mouse Embryonic Stem Cell Lines in the Study of ERK1/2 MAP Kinase Signaling Marc K. Saba-El-Leil, Christophe Frémin, and Sylvain Meloche19. 3D Organotypic Culture Model to Study Components of ERK Signaling Athina-Myrto Chioni, Rabia Tayba Bajwa, and Richard Grose20. Genetic Validation of Cell Proliferation via Ras-Independent Activation of the Raf/Mek/Erk Pathway Carmen G. Lechuga, Lucía Simón-Carrasco, Harrys K.C. Jacob, and Matthias Drosten21. Genome-Wide Analysis of RAS/ERK Signaling Targets Joshua P. Plotnik and Peter C. Hollenhorst22. Probing Chromatin Modifications in Response to ERK Signaling Ozgur Oksuz and Wee-Wei Tee23. Analyzing pERK Activation during Planarian Regeneration Susanna Fraguas, Yoshihiko Umesono, Kiyokazu Agata, and Francesc Cebrià24. Discovering Functional ERK Substrates Regulating Caenorhabditis elegans Germline Development Jessica Jie Chen and Swathi Arur25. Reconstructing ERK Signaling in the Drosophila Embryo from Fixed Images Bomyi Lim, Carmeline J. Dsilva, Ioannis G. Kevrekidis, and Stanislav Y. Shvartsman26. Drosophila Marta Forés, Aikaterini Papagianni, Laura Rodríguez-Muñoz, and Gerardo Jiménez27. Analyzing ERK Signal Dynamics during Zebrafish SomitogenesisTakaaki Matsui and Yasumasa Bessho28. Modeling RASopathies with Genetically Modified Mouse Models Isabel Hernández-Porras and Carmen Guerra29. Dissecting Cell-Fate Determination through Integrated Mathematical Modeling of the ERK/MAPK Signaling Pathway Sung-Young Shin and Lan K. Nguyen

Produktinformationen

Titel: ERK Signaling
Untertitel: Methods and Protocols
Editor:
EAN: 9781493964222
ISBN: 978-1-4939-6422-2
Format: Fester Einband
Herausgeber: Springer, Berlin
Genre: Biologie
Anzahl Seiten: 440
Gewicht: 970g
Größe: H31mm x B259mm x T182mm
Jahr: 2017
Untertitel: Englisch
Auflage: 1st ed. 2017

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