Epitope Mapping Protocols

Expert contributors from a broad spectrum of scientific backgrounds update and expand the successful first edition with cutting-edge techniques and applications, including approaches to both antibody or B-cell epitope mapping and T-cell epitope mapping.

Given the versatile utility of the determinination of epitopes, beneficial to a wide variety of scientists from immunologists to structural biologists to biotechnologists, the need for a thorough, state-of-the-art collection of experimental protocols is clear. In Epitope Mapping Protocols, Second Edition , expert contributors from a broad spectrum of scientific backgrounds update and expand the successful first edition with cutting-edge techniques and applications, including approaches to both antibody or B-cell epitope mapping and T-cell epitope mapping as well as a new section on the profiling of antibody signatures in biological fluids. Written in the popular Methods in Molecular Biology™ series format, chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls.

Comprehensive and up-to-date, Epitope Mapping Protocols, Second Edition is a reliable and valuable reference for all those who wish to understand and further investigate the diversifying field of epitope mapping.

Provides an easily accessible reference volume for epitope mapping written by leading researchers in the field Details the process of turning epitope mapping into biomarker discovery Covers a wide range of antibody-based and T-cell-based assay formats and systems Presents a modular approach to experimental design, screening modalities, detection technologies, and approaches to data analysis Comprehensive guide to developing, running, and analyzing B- and T-cell-based assays Includes supplementary material: sn.pub/extras

Inhalt
B-cell Epitope Mapping.- What Is a B-Cell Epitope?.- Whole Antigen Methods.- Structural Basis of AntibodyAntigen Interactions.- Epitope Mapping of AntibodyAntigen Complexes by Nuclear Magnetic Resonance Spectroscopy.- A Solid-Phase Mutual Inhibition Assay with Labeled Antigen.- Epitope Mapping by Surface Plasmon Resonance.- Proteolytic Fragmentation for Epitope Mapping.- Epitope Mapping by Proteolysis of AntigenAntibody Complexes.- Identifying Residues in Antigenic Determinants by Chemical Modification.- Epitope Mapping by Differential Chemical Modification of Antigens.- Peptide Library Approaches.- Linear B-Cell Epitope Mapping Using Enzyme-Linked Immunosorbent Assay for Libraries of Overlapping Synthetic Peptides.- Antibody Epitope Mapping Using SPOT™ Peptide Arrays.- Peptide Microarrays for Profiling of Modification State-Specific Antibodies.- Epitope Mapping Using Phage Display Peptide Libraries.- Antibody Epitope Mapping Using De Novo Generated Synthetic Peptide Libraries.- Antibody Specificity Profiling on Functional Protein Microarrays.- Peptide Microarrays for Determination of Cross-Reactivity.- Profiling of Antibody Signatures in Biological Fluids..- Epitope Mapping Using Randomly Generated Peptide Libraries.- Probing the Epitope Signatures of IgG Antibodies in Human Serum from Patients with Autoimmune Disease.- Microarrayed Allergen Molecules for Diagnostics of Allergy.- Monitoring B Cell Response to Immunoselected Phage-Displayed Peptides by Microarrays.- Antigen Expressed from Recombinant DNA.- Epitope Mapping Using Homolog-Scanning Mutagenesis.- Epitope Mapping by Region-Specified PCR-Mutagenesis.- Epitope Mapping Using Phage-Display Random Fragment Libraries.- B-Cell Epitope Prediction.- Prediction of Linear B-cell Epitopes.- T-Cell EpitopeMapping.- Molecular Recognition of Diverse Ligands by T-Cell Receptors.- Identification of Human MHC Class I Binding Peptides using the iTOPIA™? Epitope Discovery System.- T-Cell Epitope Mapping in Mycobacterium tuberculosis Using PepMixes Created by Micro-Scale SPOT™? Synthesis.- High-Throughput T-Cell Epitope Discovery Through MHC Peptide Exchange.- T-Cell Epitope Processing (The Epitope Flanking Regions Matter).- Identification of MHC Class II Binding Peptides: Microarray and Soluble MHC Class II Molecules.- T-Cell Epitope Mapping.- Identification and Validation of T-Cell Epitopes Using the IFN-? EliSpot Assay.