Enzyme Stabilization and Immobilization

This volume introduces the reader to the field of enzyme stabilization and the different theories of enzyme stabilization, including the use of immobilization as a stabilization technique. The first part of the book focuses on protocols for enzyme stabilization in solutions including liposome formation, micelle introduction, crosslinking, and additives. The second part of the book discusses protocols for enzyme stabilization during enzyme immobilization, including common techniques like sol-gel encapsulation, polymer encapsulation, and single enzyme nanoparticle formation. Protocols for a variety of enzymes are shown, but the enzymes are chosen as examples to show that these protocols can be used for both enzymes of biological importance, as well as enzymes of industrial importance. The final part details spectroscopic protocols, methods, and assays for studying the effectiveness of the enzyme stabilization and immobilization strategies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Enzyme Stabilization and Immobilization: Methods and Protocols, Second Edition provides molecular biologists, biochemists, and biomedical and biochemical engineers with the state-of-the-art technical information required to effectively stabilize their enzyme of interest in a variety of environments (i.e., harsh temperature, pH, or solvent conditions).

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras

Inhalt

Introduction to the Field of Enzyme Immobilization and Stabilization.- Stabilization of Enzymes through Encapsulation in Liposomes.- Micellar Enzymology for Thermal, pH, and Solvent Stability.- Lipase Activation and Stabilization in Room Temperature Ionic Liquids.- Nanoporous Gold for Enzyme Immobilization.- Enzyme Stabilization via Bio-Templated Silicification Reactions.- Covalent Immobilization of Enzymes on Eupergit ® Supports: Effect of the Immobilization Protocol.- Micellar Polymer Encapsulation of Enzymes.- Cross-Linked Enzyme Aggregates for Applications in Aqueous and Non-Aqueous Media.- Protein Coated Microcrystals, Combi-Protein Coated Microcrystals and Cross-Linked Protein Coated Microcrystals of Enzymes for Use in Low Water Media.- Macroporous Poly(GMA-co-EGMA) for Enzyme Stabilization.- Cytochrome C Stabilization and Immobilization in Aerogels.- Enzyme Immobilization and Mediation with Osmium Redox Polymers.- Ferrocene-Modified Linear Poly(ethylenimine) for Enzymatic Immobilization and Electrom Mediation.- Fad-Dependent Glucose Dehydrogenase Immobilization and Mediation within a Naphthoquinone Redox Polymer.- Layer-by-Layer Assembly of Glucose Oxidase on Carbon Nanotube Modified Electrodes.- Kinetic Measurement for Enzyme Immobilization.