Embryonic Stem Cell Therapy for Osteo-Degenerative Diseases

In this dependable and cutting-edge book, accomplished investigators provide detailed descriptions on how to expand ESCs from the most commonly used species ex vivo, i.e. mouse and human, in static culture as well as in controllable bioreactor processes.

Embryonic stem cells (ESCs) offer an unlimited self-renewing capacity, as opposed to the limits of adult stem cells; therefore, ESCs represent an almost bottomless resource for regenerative medicine and tissue engineering approaches. In Embryonic Stem Cell Therapy for Osteo-Degenerative Diseases: Methods and Protocols, accomplished investigators provide detailed descriptions on how to expand ESCs from the most commonly used species ex vivo, i.e. mouse and human, in static culture as well as in controllable bioreactor processes. The thorough and timely volume summarizes the methods that may be used to differentiate these cells along the desired lineage of choice, be it osteoblasts, osteoclasts, or chondrocytes, and consequentially also offers analysis tools for the characterization of resulting cells and evaluation of differentiation effectiveness. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls. Dependable and cutting-edge, Embryonic Stem Cell Therapy for Osteo-Degenerative Diseases: Methods and Protocols supplies the tools necessary to allow researchers to carry out critical research needed in order to bring this burgeoning and vitally important field closer to the clinic and to ensure the widespread application of a successful strategy.

Contains detailed, expert protocols specific to ESCs and stem cell differentiation Gives special care to address possible methodological pitfalls and provides trouble-shooting guides in the form of Notes sections Features contributions from renowned researchers in the field of stem cell self-renewal and the pioneers in skeletogenesis from ESCs Includes supplementary material: sn.pub/extras

Inhalt
Embryonic Stem Cells for Osteo-Degenerative Diseases.- Methods for Culturing Mouse and Human Embryonic Stem Cells.- Serum-Free and Feeder free Culture Conditions for Human Embryonic Stem Cells.- Functional Assays for Human Embryonic Stem Cell Pluripotency.- Using Cadherin Expression to Assess Spontaneous Differentiation of Embryonic Stem Cells.- Generation of Human Embryonic Stem Cells Carrying Lineage Specific Reporters.- Manipulations of MicroRNA in Human Pluripotent Stem Cells and Their Derivatives.- Large-Scale Expansion of Mouse Embryonic Stem Cells on Microcarriers.- Embryoid Body Formation: Recent Advances in Automated Bioreactor Technology.- Methods for Embryoid Body Formation: The Microwell Approach.- Human Embryonic Stem Cell-Derived Mesenchymal Progenitors: An Overview.- Derivation of Mesenchymal Stem Cells from Human Embryonic Stem Cells.- Differentiation of Human Embryonic Stem Cells into Mesenchymal Stem Cells by the Raclure Method.- Improved Media Compositions for the Differentiation of Embryonic Stem Cells into Osteoblasts and Chondrocytes.- Differentiation of Mouse Embryonic Stem Cells in Self-Assembling Peptide Scaffolds.- Methods for Investigation of Osteoclastogenesis Using Mouse Embryonic Stem Cells.- Absorption-Based Assays for the Analysis of Osteogenic and Chondrogenic Yield.- Identification of Osteoclasts in Culture.- Analysis of Glycosaminoglycans in Stem Cell Glycomics.- Drill Hole Defects: Induction, Imaging, and Analysis in the Rodent.- Measurement and Illustration of Immune Interaction after Stem Cell Transplantation.