Beschreibung

This second edition volume expands on the previous edition by presenting updated protocols for several of the techniques described in the first edition of Drosophila: Methods and Protocols and current methods that cover recent breakthroughs in Drosophila research. The book begins with a description of FlyBase--a database of genes and genomes--followed by the presentation of systems for versatile gene expression in the fly. The first few chapters in this book detail gene knockdown and editing, including CRISPR-Cas9 and protein knockdown. The next few chapters are devoted to methods describing live imaging of different tissues and organs, followed by chapters on how to quantify image data and how to probe tissue mechanics by laser ablation. The next two chapters provide methods for analyzing transcription followed by protocols to study growth, metabolism, ageing, and behavior in Drosophila. This volume concludes with chapters on electrophysiological recordings and methods to establish cell lines. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Drosophila: Methods and Protocols, Second Edition is a valuable source of hands-on protocols and reviews for molecular, cell, and developmental biologists using Drosophila as model systems in their work.

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Inhalt
1. Using FlyBase: A Database of Drosophila Genes and GenomesSteven J. Marygold, Madeline A. Crosby, Joshua L. Goodman, and The FlyBase Consortium2. The GAL4 System: A Versatile System for Manipulation and Analysis of Gene ExpressionElizabeth E. Caygill and Andrea H. Brand3. The Q-System: A Versatile Expression System for DrosophilaOlena Riabinina and Christopher J. Potter4. Analysis of MicroRNA Function in DrosophilaGeetanjali Chawla, Arthur Luhur, and Nicholas Sokol5. Methods for High-Throughput RNAi screening in Drosophila CellsMaximilian Billmann and Michael Boutros6. A Guide to Genome-Wide In Vivo RNAi Applications in DrosophilaAynur Kaya-Çopur and Frank Schnorrer7. Creating Heritable Mutations in Drosophila with CRISPR-Cas9Fillip Port and Simon L. Bullock8. Performing Chromophore-Assisted Laser Inactivation in Drosophila Embryos Using GFPAnne Pélissier-Monier, Bénédicte Sanson, and Bruno Monier9. deGradFP: A System to Knockdown GFP-Tagged ProteinsEmmanuel Caussinus and Markus Affolter10. Sample Preparation and Mounting of Drosophila Embryos for Multi-View Light Sheet MicroscopyChristopher Schmied and Pavel Tomancak11. Cultivation and Live-Imaging of Drosophila Imaginal DiscsSimon Restrepo, Jeremiah J. Zartman, and Konrad Basler12. Cultivation and Live Imaging of Drosophila OvariesMaureen Cetera, Lindsay Lewellyn, and Sally Horne-Badovinac13. Segmentation and Quantitative Analysis of Epithelial TissuesBenoit Aigouy, Daiki Umetsu, and Suzanne Eaton14. Laser Ablation to Probe the Epithelial Mechanics in DrosophilaPruthvi C. Shivakumar and Pierre-Francois Lenne15. Rapid Ovary Mass-isolation (ROMi) to Obtain Large Quantities of Drosophila Egg Chambers for Fluorescent In-Situ HybridizationHelena Jambor, Pavel Mejstrik, and Pavel Tomancak16. Chromatin Immunoprecipitation for Analyzing Transcription Factor Binding and Histone Modifications in DrosophilaYad Ghavi-Helm, Bingqing Zhao, and Eileen E.M. Furlong17. Protocols to Study Growth and Metabolism in DrosophilaKatrin Strassburger and Aurelio A. Teleman18. Protocols to Study Ageing in DrosophilaMatthew D. W. Piper and Linda Partridge19. Protocols to Study Behavior in DrosophilaWendi S. Neckameyer and Parag Bhatt20. Electrophysiological Recordings from Lobula Plate Tangential Cells in DrosophilaAlex S. Mauss and Alexander Borst21. Methods to Establish a Drosophila Cell LineAlain Debec, Timothy L. Megraw, and Antoine Guichet

Produktinformationen

Titel:	Drosophila
Untertitel:	Methods and Protocols
Editor:	Christian Dahmann
EAN:	9781493963690
ISBN:	1493963694
Format:	Fester Einband
Herausgeber:	Springer New York
Genre:	Medizin
Anzahl Seiten:	368
Gewicht:	889g
Größe:	H260mm x B183mm x T25mm
Jahr:	2016
Untertitel:	Englisch
Auflage:	2nd ed. 2016