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DNA Recombination

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Homologous recombination is important in various aspects of DNA metabolism, including damage repair, replication, telomere maintenance, and meiosis, and yeast genetics has successfully provided a framework for the mechanism of homologous recombination. Divided into four convenient sections, DNA Recombination: Methods and Protocols covers recent techniques that best utilize the advantages of the yeast system, prescribing to the belief that yeast will keep serving as a great model organism to study homologous recombination. Chapters have also been included for such exceptions as the group of genes involved in recombination that are found solely in higher eukaryotes, such as BRCA2. And looking forward, a necessary step in the direction of understanding the homologous recombination process is to isolate the machine and let it work in a test tube. Understanding the design by studying the appearance and behavior of the machinery as a single molecule will be an important milestone toward understanding the mechanism of action of the machinery. Techniques covering these topics have also been included. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, DNA Recombination: Methods and Protocols serves as an ideal guide to scientists of all backgrounds with its well-honed methodologies and strives to bring the reader to the next level of understanding regarding this vital subject.

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

Includes supplementary material: sn.pub/extras

Part I. Genetic and Molecular Biological Approaches with Yeast 1. Methods to Study Mitotic Homologous Recombination and Genome Stability Xiuzhong Zheng, Anastasiya Epstein, and Hannah L. Klein 2. Characterizing Resection at Random and Unique Chromosome Double-Strand Breaks and Telomere Ends Wenjian Ma, Jim Westmoreland, Wataru Nakai, Anna Malkova, and Michael A. Resnick 3. Characterization of Meiotic Recombination Initiation Sites Using Pulsed-field Gel Electrophoresis Sarah Farmer, Wing-Kit Leung, and Hideo Tsubouchi 4. Genome-Wide Detection of Meiotic DNA Double-Strand Break Hotspots Using Single-stranded DNA Hannah G. Blitzblau and Andreas Hochwagen 5. Detection of Covalent DNA-Bound Spo11 and Topoisomerase Complexes Edgar Hartsuiker 6. Molecular Assays to Investigate Chromatin Changes during DNA Double-Strand Break Repair in Yeast Scott Houghtaling, Toyoko Tsukuda, and Mary Ann Osley 7. Analysis of Meiotic Recombination Intermediates by Two-Dimensional Gel Electrophoresis Jasvinder A. Singh and G. Valentin Börner 8. Mapping of Crossover Sites Using DNA Microarrays Stacy Y. Chen and Jennifer C. Fung 9. Using the Semi-Synthetic Epitope System to Identify Direct Substrates of the Meiosis-Specific Budding Yeast Kinase, Mek1 Hsiao-Chi Lo and Nancy M. Hollingsworth 10. Genetic and Molecular Analysis of Mitotic Recombination in Saccharomyces cerevisiae Belén Gómez-González, José F. Ruiz, and Andrés Aguilera 11. In vivo Site-Specific Mutagenesis and Gene Collage Using the Delitto Perfetto System in Yeast Saccharomyces cerevisiae Samantha Stuckey, Kuntal Mukherjee, and Francesca Storici 12. Detection of RNA-Templated Double-Strand Break Repair in Yeast Ying Shen and Francesca Storici Part II. Genetic and Molecular Biological Approaches with Higher Eukaryotes 13. SNP-Based Mapping of Crossover Recombination in C. elegans Grace C. Bazan and Kenneth J. Hillers 14. Characterization of Meiotic Crossovers in Pollen from Arabidopsis thaliana Jan Drouaud and Christine Mézard 15. Isolation of Meiotic Recombinants from Mouse Sperm Francesca Cole and Maria Jasin 16. Homologous Recombination Assay for Interstrand Crosslink Repair Koji Nakanishi, Francesca Cavallo, Erika Brunet, and Maria Jasin 17. Evaluation of Homologous Recombinational Repair in Chicken B Lymphoma Cell Line, DT40 Hiroyuki Kitao, Seiki Hirano, and Minoru Takata 18. Understanding the Immunoglobulin Locus Specificity of Hypermutation Vera Batrak, Artem Blagodatski, and Jean-Marie Buerstedde Part III. In Vitro Reconstitution of Homologous Recombination Reactions and Single Molecular Analysis of Recombination Proteins 19. Quality Control of Purified Proteins Involved in Homologous Recombination Xiao-Ping Zhang and Wolf-Dietrich Heyer 20. Assays for Structure-Selective DNA Endonucleases William D. Wright, Kirk T. Ehmsen, and Wolf-Dietrich Heyer 21. In vitro Assays for DNA Pairing and Recombination-Associated DNA Synthesis Jie Liu, Jessica Sneeden, and Wolf-Dietrich Heyer 22. An In vitro Assay for Monitoring the Formation and Branch Migration of Holliday Junctions Mediated by a Eukaryotic Recombinase Yasuto Murayama and Hiroshi Iwasaki 23. Reconstituting the Key Steps of the DNA Double-Strand Break Repair In vitro Matthew J. Rossi, Dmitry V. Bugreev, Olga M. Mazina, and Alexander V. Mazin 24. Biochemical Studies on Human Rad51-Mediated Homologous Recombination Youngho Kwon, Weixing Zhao, and Patrick Sung 25. Studying DNA Replication Fork Stability in Xenopus Egg Extract Yoshitami Hashimoto and Vincenzo Costanzo 26. Supported Lipid-Bilayers and DNA Curtains for High-Throughput Single Molecule Studies Ilya J. Finkelstein and Eric C. Greene 27. FRET-Based Assays to Monitor DNA Binding and Annealing by Rad52 Recombination Mediator Protein Jill M. Grimme and Maria Spies 28. Visualization of Human Dmc1 Presynaptic Filaments Michael G. Sehorn and Hilarie A. Sehorn Part IV. Cell Biological Approaches to Study the In Vivo Behavior of Homologous Recombination 29. Tracking of Single and Multiple Genomic Loci in Living Yeast Cells Imen Lassadi and Kerstin Bystricky 30. Cell Biology of Homologous Recombination in Yeast Nadine Eckert-Boulet, Rodney Rothstein, and Michael Lisby 31. Live Cell Imaging of Meiotic Chromosome Dynamics in Yeast Harry Scherthan and Caroline Adelfalk 32. Chromosome Structure and Homologous Chromosome Association during Meiotic Prophase in C. elegans Kentaro Nabeshima


Titel: DNA Recombination
Untertitel: Methods and Protocols
EAN: 9781617791284
ISBN: 1617791288
Format: Fester Einband
Herausgeber: Springer-Verlag GmbH
Genre: Medizin
Veröffentlichung: 01.06.2012
Anzahl Seiten: 565
Gewicht: 1274g
Größe: H260mm x B183mm x T35mm
Jahr: 2012
Untertitel: Englisch
Auflage: 2011

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