Adaptive changes in white dead nettle leaves during micropropagation

The white dead nettle (Lamium album L.) is a herb that was successfully subjected to in vitro micropropagation followed by ex vitro adaptation back to the natural habitat. The L. album micropropagation system offers a combination of factors (light intensity, temperature, carbon dioxide level, humidity) that are limiting for plant growth and bioactive capacity. Here, we performed comparative investigation on the primary and secondary metabolism in fully expanded L. album leaves during the consecutive growth in in vivo, in vitro, and ex vitro conditions. The micropropagation did not affect the general leaf anatomy but it caused permanent decrease in the number of palisade mesophyll layers and the hydrocarbon content in the essential oils. During the in vitro conditions, the thylakoid membrane structure was altered, the photosynthetic performance was reduced, and the content of primary and secondary metabolites declined. The plant transfer back to the field led to resumption of both normal growth and general bioactive capacity. The present work broadens the understanding about the nature of plant responses towards environmental conditions.

Autorentext

Dr. Milena Angelova Dimitrova graduated MSc in Biology at the Faculty of Biology in Sofia University St. Kliment Ohridski . She continued her professional experience in the Department of Plant Physiology as assistant professor. Dr. Dimitroväs research is focused on micropropagation and studies on the biological activity of medicinal plants.