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Diagnostic Virology Protocols

  • Kartonierter Einband
  • 484 Seiten
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This second edition brings the field fully up-to-date with a focus on protocols involving nucleic acid detection, most often throu... Weiterlesen
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This second edition brings the field fully up-to-date with a focus on protocols involving nucleic acid detection, most often through some form of the polymerase chain reaction (PCR). It also covers robotics as well as future prospects.

Recent outbreaks of swine influenza and avian influenza, along with the remaining and in some cases expanding threats from HIV, dengue virus, and the viruses causing hepatitis, have reinforced the need for rapid, accurate and cost-effective diagnosis of viral disease. Diagnostic Virology Protocols, Second Edition brings the field fully up-to-date with a focus on protocols involving nucleic acid detection, most often through some form of the polymerase chain reaction (PCR). The expert contributors also delve into the key technology of robotics as well as future prospects, such as further refined point-of-care testing and the increasing importance of mathematical modelling. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Diagnostic Virology Protocols, Second Edition captures the dramatic changes in the virus diagnostic laboratory in order to better prepare scientists to combat the inevitable threats to public health from future and present infectious diseases.

From the reviews of the second edition:

In its 23 chapters it covers most viruses of interest to the diagnostic virologist. Each chapter is written by an authority in the field and is composed of a short introduction followed by a number of protocols. These are sufficiently detailed to allow the reader to set up the assays in their own lab. Most chapters address a virus type, but vary such that in some the introduction is very informative whilst in others it is merely a lead into the protocols. (Dave Yirrell, Microbiology Today, November, 2011)


1. The Detection of Adenoviruses
Kanti Pabbaraju, Sallene Wong, and Julie D. Fox

2. Alphaviruses
A.M. Powers and John T. Roehrig

3. Detection of Human Caliciviruses in Fecal Samples by RT-PCR
Robert L. Atmar, Frederick H Neill, and Françoise S. Le Guyader

4. The Detection of Dengue Virus
Nagesh K. Tripathi, Ambuj Shrivastava, Paban K. Dash, and Asha M. Jana

5. Detection of Enteroviruses from Clinical Specimens
Chit Laa Poh and Eng Lee Tan

6. The Detection of Hepatitis Viruses
Peter Karayiannis

7. Molecular Detection of Herpesviruses
Harald H. Kessler and Sophie Heyszl

8. HIV Drug Resistance Testing
Patricia Cane

9. Detection, Quantification, and Characterization of HIV/SIV
Neil Berry, Carolina Herrera, and Martin Cranage

10. Simultaneous Molecular Detection and Confirmation of Influenza AH5, with Internal Control
Joanna S. Ellis and Martin D. Curran

11. Detection of Measles, Mumps, and Rubella Viruses
Graham Tipples and Joanne Hiebert

12. Detection of High-Risk Mucosal Human Papillomavirus DNA in Human Specimens by a Novel and Sensitive Multiplex PCR Method Combined with DNA Microarray
Tarik Gheit and Massimo Tommasino

13. The Detection of Parvoviruses
Sean Doyle

14. The Detection and Characterization of Polioviruses
Javier Martin

15. Detection of Human-Pathogenic Poxviruses
Andreas Kurth and Andreas Nitsche

16. Lyssaviruses: Special Emphasis on Rabies Virus and Other Members of the Lyssavirus Genus
Graeme Harkess and Anthony R. Fooks

17. Simultaneous Detection and Differentiation of Respiratory Syncytial Virus and Other Respiratory Viral Pathogens
Susan E. Sefers, Haijing Li, and Yi-Wei Tang

18. Rotaviruses
Jim Gray and Miren Iturriza-Gómara

19. Detection of Crimean-Congo Hemorrhagic Fever, Hanta, and Sandfly Fever Viruses by Real-Time RT-PCR
Sofi M. Ibrahim, Mohamed Aitichou, Justin Hardick, Jamie Blow, Monica L. O'Guinn, and Connie Schmaljohn

20. Detection of SARS Coronavirus
J. S. Malik Peiris and Leo L.M. Poon

21. The Detection of West Nile Virus
Elizabeth B. Kauffman, Mary A. Franke, S.A. Wong, and Laura D. Kramer

22. Point of Care Testing: Diagnosis Outside the Virology Laboratory
Christopher C. Blyth, Robert Booy, and Dominic E. Dwyer

23. Modelling Emerging Viral Epidemics for Public Health Protection
Steve Leach and Ian Hall


Titel: Diagnostic Virology Protocols
EAN: 9781493958634
ISBN: 1493958631
Format: Kartonierter Einband
Herausgeber: Humana Press
Genre: Medizin
Anzahl Seiten: 484
Gewicht: 902g
Größe: H254mm x B178mm x T25mm
Jahr: 2016
Auflage: Softcover reprint of the original 2nd ed. 2011

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