Cyclin-Dependent Kinase (CDK) Inhibitors

This volume contains a collection of relevant information for drug discovery in cell cycle research. Protocols to develop screening assays or to identify novel CDK inhibitors are discussed in the first part of the book. The second part of the book describes elaborate procedures to evaluate activity and mechanism of action of new and already identified CDK inhibitors. The third part of the book talks about protocols to evaluate metabolomics changes associated to inhibitor treatment. Drug delivery strategies focused on nanoparticle development to provide alternative internalization systems for increasing inhibitor efficacy are also described. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and thorough, Cyclin-Dependent Kinase (CDK) Inhibitors: Methods and Protocols is a useful tool for scientists interested in this research field.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt

Immunoprecipitation of CDK-Cyclin Complexes for Determination of Kinase Activity.- Expression and Purification of Recombinant Cyclins and CDKs for Activity Evaluation.- Expression and Purification of Recombinant CDKs: CDK7, CDK8 and CDK9.- Preparation of CDK/Cyclin Inhibitor Complexes for Structural Determination.- Fragment-Based De Novo Design of Cyclin-Dependent Kinase 2 Inhibitors.- Protein-Protein Interaction Inspire the De Novo Design of Cyclin-Dependent Kinases Peptide Inhibitors.- Identification of Cyclin A Binders with a Fluorescent Peptide Sensor.- Cell Synchronization Techniques to Study the Action of CDK Inhibitors.- Analysis of CDK Inhibitors Action on Mitochondria-Mediated Apoptosis.- Evaluating the Effects of CDK Inhibitors in Ischemia-Reperfusion Injury Models.- Assessing Cell Cycle Independent Function of the CDK Inhibitor p21 CDKN1A in DNA Repair.- Drug Delivery Strategies of Chemical CDK Inhibitors.- Animal Models for Studying the In Vivo Functions of Cell Cycle CDKs.- Evaluating Chemical CDK Inhibitors as Cell Death Inducers.- Models for the Study of the Crosstalk between Inflammation and Cell Cycle.- Metabolomic Applications to the Characterization of the Mode-of-Action of CDK Inhibitors.