Colorectal Cancer

This volume explores the latest developments in the study of the mechanisms, diagnostics, screening methods, and therapeutics of colorectal cancer. The book's chapters are divided into three parts: the chapters in Part One examine techniques used to study the molecular mechanisms in colorectal cancer development and progression. Part Two focuses on the innovative tools used to diagnose and detect cancer lesions in the early stages of cancer. Finally, Part Three discusses recent advancements in treating colorectal tumors and identifying new therapeutic molecules for treatment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Colorectal Cancer: Methods and Protocols is a valuable resource for any scientist and researcher interested in this field of study.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Inhalt
Cell Line Models of Molecular Subtypes of Colorectal Cancer.- Dissecting Oncogenic RTK Pathways in Colorectal Cancer Initiation and Progression.- Identification of Response Elements on Promoters Using Site Directed Mutagenesis and Chromatin Immunoprecipitation.- Identification and Functional Analysis of Gene Regulatory Sequences Interacting with Colorectal Tumor Suppressors.- Methods for In Vivo Functional Studies of Chromatin-Modifying Enzymes in Early Steps of Colon Carcinogenesis.- The Colorectal Cancer Microenvironment: Strategies for Studying the Role of Cancer Associated Fibroblasts.- Methods for Assessing Apoptosis and Anoikis in Normal Intestine/Colon and Colorectal Cancer.- Molecular Analysis of the Microbiome in Colorectal Cancer.- Proteomic Analysis of Colorectal Cancer Cells.- Autophagic Flux Assessment in Colorectal Cancer Cells.- Classification of Colorectal Cancer in Molecular Subtypes by Immunohistochemistry.- Stool DNA Integrity Method for Colorectal Cancer Detection.- RT-qPCR for Fecal Mature microRNA Quantification and Validation.- A Stool Multi-Target mRNA Assay for the Detection of Colorectal Neoplasms.- Colorectal Cancer Detection Using Targeted LC-MS Metabolic Profiling.- Proteomic Profiling for Colorectal Cancer Biomarker Discovery.- Tumor-Derived Microparticles to Monitor Colorectal Cancer Evolution.- Molecular Testing for the Treatment of Advanced Colorectal Cancer: An Overview.- Testing Cell-Based Immunotherapy for Colorectal Cancer.- Patient-Derived Xenograft Models of Colorectal Cancer: Procedures for Engraftment and Propagation.- Use of Organoids to Characterize Signaling Pathways in Cancer Initiation.- Identification of Novel Molecules Targeting Cancer Stem Cells.