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This volume in the Methods in Molecular Biology(TM) series collects techniques involving claudins and the opportunity they provide... Weiterlesen
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This volume in the Methods in Molecular Biology(TM) series collects techniques involving claudins and the opportunity they provide for understanding of the molecular basis of the tight junction proteins. Includes materials lists, protocols, tips and pitfalls.

Since the initial discovery of the claudins, the field of tight junctions and cell polarity has become increasingly energized, mainly due to the fact that the claudins have provided an opportunity to understand the molecular basis of the tight junction function and their tissue specific roles. In Claudins: Methods and Protocols, experts and pioneers in the field provide an extensive collection of techniques involving these tight junction proteins. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, detailed lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Claudins: Methods and Protocols serves as an ideal guide for all scientists seeking to further our understanding of this vital area of research.

Collects key practical protocols for the study of claudins

Serves as the first comprehensive volume of techniques involving this subject

Features tips and vital implementation advice to ensure success


1. Measuring Size-Dependent Permeability of the Tight Junction Using PEG Profiling

Christina M. Van Itallie and James M. Anderson

2. Biochemical Analysis of Claudin Binding Compatibility

Christina Ward and Michael Koval

3. Electrophysiological Characterization of Claudin Ion Permeability Using Stably Transfected Epithelial Cell Lines

Alan S.L. Yu

4. The Tight Junction, Intercellular Seal as a Cell Signaling Player: Protocols for Examination of Its Status

Makoto Osanai

5. Interactions between Clostridium perfringens Enterotoxin and Claudins

Susan L. Robertson and Bruce A. McClane

6. Biophysical Methods to Probe Claudin Mediated Adhesion at the Cellular and Molecular Level

Sri Ram Krishna Vedula, Tong Seng Lim, Walter Hunziker, and Chwee Teck Lim

7. Detection of Tight Junction Barrier Function In Vivo by Biotin

Lei Ding, Yuguo Zhang, Rodney Tatum, and Yan-Hua Chen

8. The Co-Culture Method to Examine Interactions between Claudin Isoforms in Tight Junction-Free HEK293 Cells and Tight Junction-Bearing MDCK II Cells

Tetsuichiro Inai

9. Claudin 4: Functional Studies Beyond the Tight Junction

Holly A. Eckelhoefer, Thejani E. Rajapaksa, Jing Wang, Mary Stover-Hamer, Nancy C. Appleby, Jun Ling, and David D. Lo

10. Methods to Analyze Subcellular Localization and Intracellular Trafficking of Claudin 16

P. Jaya Kausalya and Walter Hunziker

11. Claudin Family Proteins in C. elegans

Jeffrey S.Simske and Jeff Hardin

12. In Vivo Imaging of Tight Junctions Using Claudin-EGFP Transgenic Medaka

Tatsuo Miyamoto, Mikio Furuse, and Makoto Furutani-Seiki

13. Claudins in a Primary Cultured Puffer Fish (Tetraodon nigroviridis) Gill Epithelium

Phuong Bui and Scott P. Kelly

14. Manipulating Claudin Expression in Avian Embryos

Michelle M. Collins and Aimee K. Ryan

15. Identification of Claudins by Western Blot and Immunofluorescence in Different Cell Lines and Tissues

Lorenza González-Mariscal, Erika Garay, and Miguel Quirós

16. Expression and Function of Claudins in Hepatocytes

Takashi Kojima and Norimasa Sawada

17. Analysis of Changes in the Expression Pattern of Claudins Using Salivary Acinar Cells in Primary Culture

Junko Fujita-Yoshigaki

18. Development of Biological Tools to Study Claudins in the Male Reproductive Tract

Daniel G. Cyr, Évemie Dubé, Julie Dufresne, and Mary Gregory

19. Using Molecular Tracers to Assess the Integrity of the Intestinal Epithelial Barrier In Vivo

Julian A. Guttman

20. Laboratory Methods in the Study of Endometrial Claudin-4

Paulo Serafini, André Monteiro Rocha, Gary Daniel Smith, Eduardo Leme Alves da Motta, and Edmund Chada Baracat

21. Role of Claudins in Oxidant-Induced Alveolar Epithelial Barrier Dysfunction

Yu Sun, Richard D. Minshall, and Guochang Hu

22. Tracing the Endocytosis of Claudin-5 in Brain Endothelial Cells

Svetlana M. Stamatovic, Richard F. Keep, and Anuska V. Andjelkovic

23. Quantitative In Situ Analysis of Claudin Expression at the Blood-Retinal Barrier

Heping Xu and Janet Liversidge

24. MMP-Mediated Disruption of Claudin-5 in the Blood-Brain Barrier of Rat Brain after Cerebral Ischemia

Yi Yang and Gary A. Rosenberg

25. Claudin-5 Expression in In Vitro Models of the Blood-Brain Barrier

Itzik Cooper, Katayun Cohen-Kashi-Malina, and Vivian I. Teichberg

26. HIV-1-Induced Alterations of Claudin-5 Expression at the Blood Brain Barrier Level

Ibolya E. András and Michal Toborek

27. Enhanced Immunohistochemical Resolution of Claudin Proteins in Glycolmethacrylate-Embedded Tissue Biopsies

Jane E. Collins, Adam Kirk, Sara K. Campbell, Juan Mason, and Susan J. Wilson

28. Claudin-16/Paracellin-1, Cloning, Expression, and Its Role in Tight Junction Functions in Cancer and Endothelial Cells

Tracey A. Martin and Wen G. Jiang

29. Dynamics of Claudins Expression in Colitis and Colitis-Associated Cancer in Rat

Yoshiaki Arimura, Kanna Nagaishi, and Masayo Hosokawa

30. Anti-Claudin 4 Conjugated Highly Luminescence Nanoparticles as Biological Labels for Pancreatic Cancer Sensing

Ken-Tye Yong


Titel: Claudins
Untertitel: Methods and Protocols
EAN: 9781617791840
ISBN: 1617791849
Format: Fester Einband
Herausgeber: Humana Press
Anzahl Seiten: 464
Gewicht: 1063g
Größe: H260mm x B183mm x T29mm
Jahr: 2011
Untertitel: Englisch
Auflage: 2011

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