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Chromosome and Genomic Engineering in Plants

  • Fester Einband
  • 232 Seiten
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This volume assembles protocols for chromosome engineering and genome editing in two recently developed approaches for manipulating chromosomal and genomic DNA in plants. The first approach is a plant chromosome vector system, which allows the introduction of desired genes or DNA into target sites on the chromosome vector, particularly by sequence-specific recombination. The second approach is genome-editing, which makes it possible to introduce mutations into any of the genes of DNA that we wish to change. In addition, this book also covers other related techniques used to accelerate progress in plant chromosome and genome engineering. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Chromosome and Genomic Engineering in Plants: Methods and Protocols provides a comprehensive source of protocols and other necessary information to anyone interested in this field of study.

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

1. Production of Engineered Minichromosome Vectors via the Introduction of Telomere SequencesNathaniel Graham, Nathan Swyers, Jon Cody, Morgan McCaw, Changzeng Zhao, and James A. Birchler2. Method for Biolistic Site-Specific Integration in Rice and Tobacco Catalyzed by Bxb1 Integrase Ruyu Li, Zhiguo Han, Lili Hou, Gurminder Kaur, Qian Yin, and David W. Ow3. Protocol for In Vitro Stacked Molecules Compatible with In Vivo Recombinase Mediated Gene Stacking Weiqiang Chen and David W. Ow4. Generation and Analysis of Transposon Ac/Ds-Induced Chromosomal Rearrangements in Rice Plants Yuan Hu Xuan, Thomas Peterson, and Chang-deok Han5. One-Step Generation of Chromosomal Rearrangements in Rice Minoru Murata, Asaka Kanatani, and Kazunari Kashihara6. Genome Elimination by Tailswap CenH3: In Vivo Haploid Production in Arabidopsis thaliana Ravi Maruthachalam and Ramesh Bondada7. Gametocidal Systems for Dissecting Wheat ChromosomesHisashi Tsujimoto8. CRISPR/Cas-Mediated Site-Specific Mutagenesis in Arabidopsis thaliana Using Cas9 Nucleases and Paired Nickases Simon Schiml, Friedrich Fauser, and Holger Puchta9. Targeted Mutagenesis in Rice Using and TALENs and the CRISPR/Cas9 SystemMasaki Endo, Ayako Nishizawa-Yokoi, and Seiichi Toki10. Seamless Genome Editing in Rice via Gene Targeting and Precise Marker Elimination Ayako Nishizawa-Yokoi, Hiroaki Saika, and Seiichi Toki11. Development of Genome Engineering Tools from Plant-Specific PPR Proteins Using Animal Cultured CellsTakehito Kobayashi, Yusuke Yagi, and Takahiro Nakamura12. Chromosomal Allocation of DNA Sequences in Wheat Using Flow-Sorted ChromosomesPetr Cápal, Jan Vrána, Marie Kubaláková, Takashi R. Endo, and Jaroslav Doleel13. Image Analysis of DNA Fiber and Nucleus in PlantsNobuko Ohmido, Toshiyuki Wako, Seiji Kato, and Kiichi Fukui14. Detection of Transgenes on DNA Fibers Fukashi Shibata 15. Three-Dimensional, Live-Cell Imaging of Chromatin Dynamics in Plant Nuclei Using Chromatin Tagging Systems Takeshi Hirakawa and Sachihiro Matsunaga16. Chromatin Immunoprecipitation for Detecting Epigenetic Marks on Plant Nucleosomes Kiyotaka Nagaki17. Mapping of T-DNA and Ac/Ds by TAIL-PCR to Analyze Chromosomal Rearrangements< Satoru Fujimoto, Sachihiro Matsunaga, and Minoru Murata


Titel: Chromosome and Genomic Engineering in Plants
Untertitel: Methods and Protocols
EAN: 9781493949298
ISBN: 1493949292
Format: Fester Einband
Herausgeber: Springer New York
Anzahl Seiten: 232
Gewicht: 642g
Größe: H260mm x B183mm x T18mm
Jahr: 2016
Untertitel: Englisch
Auflage: 1st ed. 2016

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