Chikungunya Virus

This volume covers multidisciplinary approaches on various aspects of Chikungunya Virus (CHIKV) research that was brought together from leading laboratories across the globe. Topics covered include techniques in clinical and diagnostic virology, basic protocols in cell and virus culture, bioinformatics, and proteomics approaches in cellular response studies. Chikungunya Virus: Methods and Protocols also discusses methods in immunology and animal model studies, as well as different strategies of antivirals and vaccine development for therapeutics against CHIKV infection. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary material and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and practical, Chikungunya Virus: Methods and Protocols is a useful and comprehensive resource for CHIKV researchers and anyone interested in learning more about the Chikungunya Virus.

Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts Includes supplementary material: sn.pub/extras

Inhalt

Evolution and Epidemiology of Chikungunya Virus.- Detection and Quantification of Chikungunya Virus by Real-Time RT-PCR Assay.- Advanced Genetic Methodologies in Tracking Evolution and Spread of Chikungunya Virus.- Synthetic Peptide-Based Antibody Detection for Diagnosis of Chikungunya Infection with and without Neurological Complications.- Expression and Purification of E2 Glycoprotein from Insect Cells (Sf9) for Use in Serology.- Diagnostic Methods for CHIKV Based on Serological Tools.- Utilization and Assessment of Throat Swab and Urine Specimens for Diagnosis of Chikungunya Virus Infection.- Propagation of Chikungunya Virus Using Mosquito Cells.- Infectious Viral Quantification of Chikungunya Virus - Virus Plaque Assay.- Molecular Epidemiology of Chikungunya Virus by Sequencing.- Chikungunya Virus Infection of Aedes Mosquitoes.- Analysis of CHIKV in Mosquitoes Infected via Artificial Blood Meal.- Chikungunya Virus Growth and Fluorescent Labeling: Detection of Chikungunya Virus by Immunofluorescence Assay.- Virus Isolation and Preparation of Sucrose-Banded Chikungunya Virus Samples for Transmission Electron Microscopy.- Viral-Host Protein Interaction Studies Using Yeast Two-Hybrid Screening Method.- Application of GelC-MS/MS to Proteomic Profiling of Chikungunya Virus Infection: Preparation of Peptides for Analysis.- Bioinformatics Based Approaches to Study Virus-Host Interactions During Chikungunya Virus Infection.- T-Cell Epitope Prediction of Chikungunya Virus.- Mouse Models of Chikungunya Virus.- Generation of Mouse Monoclonal Antibodies Specific to Chikungunya Virus Using ClonaCell-HY Hybridoma Cloning Kit.- Immunohistochemical Detection of Chikungunya Virus Antigens in Formalin-Fixed and Paraffin-Embedded Tissues.- Antiviral Strategies against Chikungunya Virus.- A Real Time Cell Analyzing Assay for Identification of Novel Antiviral Compounds against Chikungunya Virus.- Using Bi-Cistronic Baculovirus Expression Vector System to Screen the Compounds that Interfere the Infection of Chikungunya Virus.- Neutralization Assay for Chikungunya Virus Infection: Plaque Reduction Neutralization Test.- Reverse Genetics Approaches for Chikungunya Virus.- Production of Chikungunya Virus-Like Particles and Subunit Vaccines in Insect Cells.- Protocols for Developing Novel Chikungunya Virus DNA Vaccines.