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This second edition expands on the first edition with new chapters describing methods for studying cell movement, molecular compon... Weiterlesen
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This second edition expands on the first edition with new chapters describing methods for studying cell movement, molecular components involved in chemotaxis, spatiotemporal dynamics of signaling components, and quantitative modeling, as well as several updated chapters from the first edition. Various methods to investigate directional cell growth and movements are presented in Chapters 1-20. These chapters contains experimental procedures to visualize and measure migration behaviors of different kinds of organisms, including chemotropism in the budding yeast; cell growth and migration of D. discoideum; border cell migration in Drosophila; chemotaxis of mouse and human neutrophils; and HIV-induced T cell chemotactic response. Chemotaxis: Methods and Protocols, Second Edition also contains microscopy procedures for studying breast cancer cell migration, tumor cell invasion in vivo, and axon guidance. The book concludes with Chapters 21-27 describing methods that measure spatiotemporal dynamics of signaling components involved in chemotaxis; introduce imaging techniques, such as TRIF, BRET, FRET, and single-molecule microscopy; and mathematical models of experimentally generated chemoattractant gradients. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting edge and thorough, Chemotaxis: Methods and Protocols, Second Edition is a valuable resource for anyone who is interested in the diverse methodologies that are propelling chemotaxis research forward.

Includes cutting-edge methods and protocols

Provides step-by-step detail essential for reproducible results

Contains key notes and implementation advice from the experts

1. In Situ Assays of Chemotropism during Yeast Mating David E. Stone and Robert A. Arkowitz2. Imaging Polarization in Budding Yeast Allison W. McClure, Chi-Fang Wu, Sam A. Johnson, and Daniel J. Lew3. Migration of Dictyostelium discoideum to the Chemoattractant Folic Acid Karl J. Aufderheide and Chris Janetopoulos4. Mitochondrial Stress Tests Using Seahorse Respirometry on Intact Dictyostelium discoideum Cells Sui Lay, Oana Sanislav, Sarah J. Annesley, and Paul R. Fisher5. Studying Chemoattractant Signal Transduction Dynamics in Dictyostelium by BRET A.F.M. Tariqul Islam, Branden M. Stepanski, and Pascale G. Charest6. Wave Patterns in Cell Membrane and Actin Cortex Uncoupled from Chemotactic Signals Günther Gerisch and Mary Ecke7. Chemotactic Blebbing in Dictyostelium Cells Evgeny Zatulovskiy and Robert R. Kay8. Dissecting Spatial and Temporal Sensing in Dictyostelium Chemotaxis Using a Wave Gradient Generator Akihiko Nakajima and Satoshi Sawai9. Employing Dictyostelium as an Advantageous 3Rs Model for Pharmacogenetic Research Grant P. Otto, Marco Cocorocchio, Laura Munoz, Richard A. Tyson, Till Bretschneider, and Robin S.B. Williams10. Identification of Associated Proteins by Immunoprecipitation and Mass Spectrometry Analysis Xiumei Cao and Jianshe Yan11. Biochemical Responses to Chemically Distinct Chemoattractants during the Growth and Development of Dictyostelium Netra Pal Meena and Alan R. Kimmel12. Live Imaging of Border Cell Migration in Drosophila Wei Dai and Denise J. Montell13. ShRNA-Induced Gene Knock-Down In Vivo to Investigate Neutrophil Function Abdul Basit, Wenwen Tang, and Dianqing Wu14. Studying Neutrophil Migration In Vivo Using Adoptive Cell Transfer Yoshishige Miyabe, Nancy D. Kim, Chie Miyabe, and Andrew D. Luster15. Intravital Two-Photon Imaging of Lymphocytes Crossing High Endothelial Venules and Cortical Lymphatics in the Inguinal Lymph Node Chung Park, Il-Young Hwang, and John H. Kehrl 16. Flow Cytometry-Based Quantification of HIV-Induced T Cell Chemotactic Response Yuntao Wu17. Visualizing Cancer Cell Chemotaxis and Invasion in 2D and 3DOlivia Susanto, Andrew J. Muinonen-Martin, Max Nobis, and Robert H. Insall18. 4D Tumorigenesis Model for Quantitating Coalescence, Directed Cell Motility and Chemotaxis, Identifying Unique Cell Behaviors and Testing Anti-Cancer DrugsSpencer Kuhl, Edward Voss, Amanda Scherer, Daniel F. Lusche, Deborah Wessels, and David R. Soll19. An Experimental Model for Simultaneous Study of Migration of Cell Fragments, Single Cells and Cell SheetsYao-Hui Sun, Yuxin Sun, Kan Zhu, Bruce W. Draper, Alex Mogilner, and Min Zhao 20. Axon Guidance Studies Using a Microfluidics-Based Chemotropic Gradient Generator Zac Pujic, Huyen Nguyen, Nick Glass, Justin Cooper-White, and Geoffrey J. Goodhill 21. Visualization of Actin Assembly and Filament Turnover by In Vitro Multi-Color TIRF Microscopy Moritz Winterhoff, Stefan Brühmann, Christof Franke, Dennis Breitsprecher, and Jan Faix22. Quantitative Monitoring Spatiotemporal Activation of Ras and PKD1 Using Confocal Fluorescent MicroscopyXuehua Xu, Michelle Yun, Xi Wen, Joseph Brzostowski, Wei Quan, Q. Jane Wang, and Tian Jin 23. Fluorescence Readout of a Patch Clamped Membrane by Laser Scanning Microscopy Matthias Gerhardt, Michael Walz, and Carsten Beta 24. Use of Resonance Energy Transfer Techniques for In Vivo Detection of Chemokine Receptor Oligomerization Laura Martínez-Muñoz, José Miguel Rodríguez-Frade, and Mario Mellado 25. Multi-State Transition Kinetics of Intracellular Signaling Molecules by Single-Molecule Imaging Analysis Satomi Matsuoka, Yukihiro Miyanaga, and Masahiro Ueda 26. Mathematics of Experimentally Generated Chemoattractant Gradients Marten Postma and Peter J.M. van Haastert 27. Modeling Excitable Dynamics of Chemotactic Networks Sayak Bhattacharya and Pablo A. Iglesias


Titel: Chemotaxis
Untertitel: Methods and Protocols
EAN: 9781493934782
ISBN: 978-1-4939-3478-2
Format: Fester Einband
Herausgeber: Springer, Berlin
Genre: Biologie
Anzahl Seiten: 425
Gewicht: g
Größe: H30mm x B262mm x T185mm
Jahr: 2016
Auflage: 2. Aufl.

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